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Applied Biochemistry and Biotechnology

, Volume 169, Issue 4, pp 1329–1345 | Cite as

Metal-Based Biologically Active Compounds: Synthesis, Characterization, DNA Interaction, Antibacterial, Cytotoxic and SOD Mimic Activities

  • Mohan N. Patel
  • Chintan R. Patel
  • Hardik N. Joshi
Article

Abstract

The square pyramidal copper(II) complexes of N, O- donor ligand and ciprofloxacin have been synthesized. Synthesized complexes were characterized by physicochemical parameters like elemental analysis, electronic, FT–IR and LC–MS spectra. The complexes were screened for their antimicrobial activity against Gram(+Ve), i.e. Staphylococcus aureus, Bacillus subtilis, and Gram(−Ve), i.e. Serratia marcescens, Pseudomonas aeruginosa and Escherichia coli, microorganisms in terms of minimum inhibitory concentration and colony-forming unit. To determine the binding mode of complexes with Herring Sperm DNA, absorption titration and viscosity measurement were employed. DNA cleavage activity was carried out by gel electrophoresis experiment using supercoiled form of pUC19 DNA. The complexes were tested for their superoxide dismutase mimic activity in terms of IC50 value. Synthesized complexes were also screened for their cytotoxicity using brine shrimp lethality assay method.

Keywords

Antimicrobial activity DNA binding DNA cleavage SOD mimic Cytotoxicity 

Notes

Acknowledgments

Authors thank the Head of the Department of Chemistry, Sardar Patel University, India for making it convenient to work in the laboratory and U.G.C. for providing financial support under “UGC Research Fellowship in Science for Meritorious Students” scheme.

Supplementary material

12010_2012_86_MOESM1_ESM.docx (1.2 mb)
ESM 1 (DOCX 1.21 mb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Mohan N. Patel
    • 1
  • Chintan R. Patel
    • 1
  • Hardik N. Joshi
    • 1
  1. 1.Department of ChemistrySardar Patel UniversityVallabh VidyanagarIndia

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