Applied Biochemistry and Biotechnology

, Volume 169, Issue 4, pp 1405–1417 | Cite as

Analysis of Dormant Bud (Banjhi) Specific Transcriptome of Tea (Camellia sinensis (L.) O. Kuntze) from cDNA Library Revealed Dormancy-Related Genes

  • Krishnaraj Thirugnanasambantham
  • Gajjeraman Prabu
  • Senthilkumar Palanisamy
  • Suresh Ramraj Subhas Chandrabose
  • Abul Kalam Azad Mandal
Article

Abstract

Bud dormancy is of ecological and economical interest due to its impact on tea (Camellia sinensis (L.) O. Kuntze) plant growth and yield. Growth regulation associated with dormancy is an essential element in plant’s life cycle that leads to changes in expression of large number of genes. In order to identify and provide a picture of the transcriptome profile, cDNA library was constructed from dormant bud (banjhi) of tea. Sequence and gene ontology analysis of 3,500 clones, in many cases, enabled their functional categorization concerning the bud growth. Based on the cDNA library data, the putative role of identified genes from tea is discussed in relation to growth and dormancy, which includes morphogenesis, cellular differentiation, tropism, cell cycle, signaling, and various metabolic pathways. There was a higher representation of unknown processes such as unknown molecular functions (65.80 %), unknown biological processes (62.46 %), and unknown cellular components (67.42 %). However, these unknown transcripts represented a novel component of transcripts in tea plant bud growth and/or dormancy development. The identified transcripts and expressed sequence tags provides a valuable public resource and preliminary insights into the molecular mechanisms of bud dormancy regulation. Further, the findings will be the target of future expression experiments, particularly for further identification of dormancy-related genes in this species.

Keywords

Banjhi Bud cDNA Dormancy Library Tea Transcriptome 

Notes

Acknowledgments

Authors are thankful to Dr. P. Mohan Kumar, Director, UPASI Tea Research Foundation, and Dr. N. Muraleedharan, Tea Research Association, Toklai for their encouragement and support during the course of study. Financial assistance from NTRF, Tea Board, Kolkata is also gratefully acknowledged.

Supplementary material

12010_2012_70_MOESM1_ESM.xls (186 kb)
Supplementary Table 1A table containing the banjhi bud specific cDNA clone number as well as their accession number, the putative identities of, the top BLAST result, the e-value for the BLAST hit, the GenBank accession numbers and the molecular, functional, cellular component categorization, is available online. (XLS 185 kb)

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Krishnaraj Thirugnanasambantham
    • 1
  • Gajjeraman Prabu
    • 1
    • 2
  • Senthilkumar Palanisamy
    • 1
  • Suresh Ramraj Subhas Chandrabose
    • 1
  • Abul Kalam Azad Mandal
    • 1
    • 3
  1. 1.UPASI-Tea Research FoundationValparaiIndia
  2. 2.Department of BiotechnologyKarpagam UniversityCoimbatoreIndia
  3. 3.School of Bio Sciences and Technology (SBST), Plant Biotechnology DivisionVIT UniversityVelloreIndia

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