Effect of Physicochemical Characteristics of Cellulosic Substrates on Enzymatic Hydrolysis by Means of a Multi-Stage Process for Cellobiose Production
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The effect of two types of cellulose, microcrystalline cellulose and paper pulp, on enzymatic hydrolysis for cellobiose production was investigated. The particle size, the relative crystallinity index and the water retention value were determined for both celluloses. A previously studied multistage hydrolysis process that proved to enhance the cellobiose production was studied with both types of celluloses. The cellobiose yield exhibited a significant improvement (120% for the microcrystalline cellulose and 75% for the paper pulp) with the multistage hydrolysis process compared to continuous hydrolysis. The conversion of cellulose to cellobiose was greater for the microcrystalline cellulose than for the paper pulp. Even with high crystallinity, microcrystalline cellulose achieved the highest cellobiose yield probably due to its highest specific surface area accessible to enzymes and quantity of adsorbed protein.
KeywordsCellobiose Multistage process Cellulase binding Water retention value Microcrystalline cellulose Paper pulp
This study was financially supported by the Walloon Region (TECHNOSE project no. 716757). We thank Ms. Virginie Byttebier for her excellent assistance.
- 1.Smits, G., Coussement, P., & De Leenheer, L. (1993). European Patent EP 0 354 199 B1.Google Scholar
- 2.Yamasaki, N., Ibuki, I. and Isaka, K. (2007) Patent Application Publication. US 2007/0207108 A1.Google Scholar
- 3.Franklin, K. R., Hopkinson, A., Webb, N. and White, M. S. (2002) Patent WO/2002/032914.Google Scholar
- 5.Singer, N. S., Dubois, G. E., & Muller, G. W. (1990). European Patent EP 0 355 138.Google Scholar
- 12.Van Soest, P. J., & Wine, R. H. (1967). Journal of the Association of Official Analytical Chemists, 50, 50–55.Google Scholar
- 14.Adey, B. and Baker, J.(1996) NREL Laboratory analytical procedure. Measurement of cellulases activities. Available from http://www.nrel.gov/biomass/pdfs/42628.pdf. Accessed January 15, 2011.
- 22.Stone, J. E., Scallan, A. M., Donefer, E., & Ahlgren, E. (1969). Advances in Chemistry Series, 95, 219–241.Google Scholar