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Applied Biochemistry and Biotechnology

, Volume 166, Issue 5, pp 1137–1147 | Cite as

Amentoflavone Inhibits UVB-Induced Matrix Metalloproteinase-1 Expression Through the Modulation of AP-1 Components in Normal Human Fibroblasts

  • Chan-Woo Lee
  • Yongjoo Na
  • Nok-hyun Park
  • Han-Sung Kim
  • Soo Mi Ahn
  • Jin Woong KimEmail author
  • Han-Kon Kim
  • Young Pyo JangEmail author
Article

Abstract

Amentoflavone is a well-known biflavonoid that has diverse biological effects. Previously, we reported that amentoflavone suppressed UVB-induced matrix metalloproteinase-1 (MMP-1) expression in normal human fibroblasts (NHF). We investigated the effects of amentoflavone on UVB-induced MMP-1 expression in order to elucidate its mode of action. NHF were treated with amentoflavone for indicated times and doses with UVB irradiation. The expressions of MMP-1 gene and protein were determined by RT-PCR and ELISA, respectively. MAP kinase phosphorylation and the expression of c-Fos protein were determined by Western blot. The treatment of amentoflavone completely blocked the upregulation of MMP-1 which is induced by UVB irradiation in HaCaT–NHF co-culture in a dose-dependent manner as well as in NHF monoculture. Also, amentoflavone inhibited UVB-induced activation of extracellular signal-regulated kinase (ERK) without changing total ERK protein level, and did not affect p38 or JNK activation. Finally, AP-1 transcription factor components, phospho-c-Jun and c-Fos protein expressions were decreased by amentoflavone treatment. The major finding of this study shows that amentoflavone inhibits intracellular cell signaling ERK pathway leading to the prevention of MMP-1 expression in human skin fibroblasts. Therefore, these results strongly suggest that amentoflavone should be investigated as a potential agent for the prevention and the treatment of skin photoaging.

Keywords

Amentoflavone Ultraviolet-B Matrix metalloproteinase-1 MAP kinase AP-1 Human fibroblasts 

Notes

Acknowledgement

The study was financially supported by the research fund of Hanyang University (HY-2011-N).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Chan-Woo Lee
    • 1
  • Yongjoo Na
    • 1
  • Nok-hyun Park
    • 1
  • Han-Sung Kim
    • 1
  • Soo Mi Ahn
    • 1
  • Jin Woong Kim
    • 2
    Email author
  • Han-Kon Kim
    • 1
  • Young Pyo Jang
    • 3
    • 4
    Email author
  1. 1.Amore-Pacific Co. R&D CenterYongin-siRepublic of Korea
  2. 2.Department of Applied ChemistryHanyang UniversityAnsanRepublic of Korea
  3. 3.Division of Pharmacognosy, Department of Oriental Pharmaceutical Science, College of PharmacyKyung Hee UniversitySeoulRepublic of Korea
  4. 4.Department of Life and Nanopharmaceutical Sciences, College of PharmacyKyung Hee UniversitySeoulRepublic of Korea

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