Interspecies Interaction of Signal Peptide PapR Secreted by Bacillus cereus and Its Effect on Production of Antimicrobial Peptide
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This study was carried out to investigate the interspecies interaction of PapR peptide secreted by Bacillus cereus on production of BSAP-254, an antimicrobial peptide produced by Bacillus subtilis SC-8 isolated from the Korean fermented soybean paste and exhibited narrow antagonistic activity against the B. cereus group, but not against other foodborne pathogens. PapR is a signal peptide that activates PlcR, which is a pleiotropic regulator controlling the expression of various virulence factors in B. cereus. When B. subtilis SC-8 was co-cultured with B. cereus, it completely inhibited the growth of B. cereus within 12 h, and the rate of BSAP-254 production was increased 34.2% at 12 h. Furthermore, 5 μM of synthetic PapR peptide added to the culture of B. subtilis SC-8 increased the rate of BSAP-254 production up to 59.7%. The growth of B. subtilis SC-8, however, was not significantly different with or without the addition of PapR. When B. cereus papR mutant was co-cultured with B. subtilis SC-8, the growth of the mutant was not inhibited and the rate of BSAP-254 production was decreased by 45%.
KeywordsBacillus cereus group PapR Bacillus subtilis Antimicrobial peptide Interspecies interaction
We thank Dr. Andrei P. Pomerantsev and Dr. Stephen H. Leppla (National Institutes of Health, Bethesda, MD, USA) for the gift of B. cereus papR mutant strain. This study was financially supported by the Basic Research Program (no. 2009–0073489) through the National Research Foundation of Korea (NRF) supported by the Ministry of Education, Science and Technology.
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