Applied Biochemistry and Biotechnology

, Volume 166, Issue 3, pp 700–710 | Cite as

Interspecies Interaction of Signal Peptide PapR Secreted by Bacillus cereus and Its Effect on Production of Antimicrobial Peptide

  • In-Cheol Yeo
  • Nam Keun Lee
  • Chang-Jun Cha
  • Young Tae Hahm
Article

Abstract

This study was carried out to investigate the interspecies interaction of PapR peptide secreted by Bacillus cereus on production of BSAP-254, an antimicrobial peptide produced by Bacillus subtilis SC-8 isolated from the Korean fermented soybean paste and exhibited narrow antagonistic activity against the B. cereus group, but not against other foodborne pathogens. PapR is a signal peptide that activates PlcR, which is a pleiotropic regulator controlling the expression of various virulence factors in B. cereus. When B. subtilis SC-8 was co-cultured with B. cereus, it completely inhibited the growth of B. cereus within 12 h, and the rate of BSAP-254 production was increased 34.2% at 12 h. Furthermore, 5 μM of synthetic PapR peptide added to the culture of B. subtilis SC-8 increased the rate of BSAP-254 production up to 59.7%. The growth of B. subtilis SC-8, however, was not significantly different with or without the addition of PapR. When B. cereus papR mutant was co-cultured with B. subtilis SC-8, the growth of the mutant was not inhibited and the rate of BSAP-254 production was decreased by 45%.

Keywords

Bacillus cereus group PapR Bacillus subtilis Antimicrobial peptide Interspecies interaction 

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • In-Cheol Yeo
    • 1
  • Nam Keun Lee
    • 1
  • Chang-Jun Cha
    • 1
  • Young Tae Hahm
    • 1
  1. 1.Department of Biotechnology (BK21 Program)Chung-Ang UniversityAnseongRepublic of Korea

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