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Applied Biochemistry and Biotechnology

, Volume 166, Issue 2, pp 379–388 | Cite as

Pathophysiology of Microwave Radiation: Effect on Rat Brain

  • Kavindra Kumar Kesari
  • Sanjay Kumar
  • Jitendra Behari
Article

Abstract

The study aims to investigate the effect of 2.45 GHz microwave radiation on Wistar rats. Rats of 35 days old with 130 ± 10 g body weight were selected for this study. Animals were divided into two groups: sham exposed and experimental (six animals each). Animals were exposed for 2 h a day for 45 days at 2.45 GHz frequency (power density, 0.21 mW/cm2). The whole body specific absorption rate was estimated to be 0.14 W/kg. Exposure took place in a ventilated plexiglas cage and kept in an anechoic chamber under a horn antenna. After completion of the exposure period, rats were killed, and pineal gland and whole brain tissues were isolated for the estimation of melatonin, creatine kinase, caspase 3, and calcium ion concentration. Experiments were performed in a blind manner and repeated. A significant decrease (P < 0.05) was recorded in the level of pineal melatonin of exposed group as compared with sham exposed. A significant increase (P < 0.05) in creatine kinase, caspase 3, and calcium ion concentration was observed in whole brain of exposed group of animals as compared to sham exposed. One-way analysis of variance method was adopted for statistical analysis. The study concludes that a reduction in melatonin or an increase in caspase-3, creatine kinase, and calcium ion may cause significant damage in brain due to chronic exposure of these radiations. These biomarkers clearly indicate possible health implications of such exposures.

Keywords

Melatonin Caspase-3 Creatine kinase Calcium ion concentration Microwave 

Notes

Acknowledgment

Authors are thankful to the Indian Council for Medical Research (ICMR), New Delhi, for the financial assistance. Help of Mr. Rajesh Kumar Kushwaha during AAS analysis is thankfully acknowledged.

Conflict of interest

The authors have no conflicts of interest. They alone are responsible for the content and writing of the paper.

References

  1. 1.
    Ismail, N. H., & Ibrahim, A. T. (2002). Temperature distribution in the human brain during ultrasound hyperthermia. Journal of Electromagnetic Waves and Applications, 16, 803–811.CrossRefGoogle Scholar
  2. 2.
    Barnett, J., Timotijevic, L., Shepherd, R., & Senior, V. (2007). Public responses to precautionary information from the Department of Health (UK) about possible health risks from mobile phones. Health Policy, 82, 240–250.CrossRefGoogle Scholar
  3. 3.
    Kang, X. K., Li, L. W., Leong, M. S., & Kooi, P. S. (2001). A method of moments study of SAR inside spheroidal human head and current distribution among handset wireless antennas. Journal of Electromagnetic Waves and Applications, 15, 61.CrossRefGoogle Scholar
  4. 4.
    Dimbylow, P. J., & Mann, S. M. (1994). SAR calculations in an anatomically realistic model of the head for mobile communication transceivers at 900 MHz and 1.8 GHz. Physics in Medicinal Biology, 39, 1537–1544.CrossRefGoogle Scholar
  5. 5.
    Rothman, K. J., Chou, C. K., Morgan, R., Balzano, Q., Guy, A. W., & Funch, D. P. (1996). Assessment of cellular telephone and other radio frequency exposure for epidemiologic research. Epidemiology, 7, 291–298.CrossRefGoogle Scholar
  6. 6.
    Ferreri, F. G., Curico, P., Pasqualetti, L., de Gennaro, L., Fini, R., & Rossini, P. M. (2006). Mobile phone emissions and human brain excitability. Annals of Neurology, 60, 188.CrossRefGoogle Scholar
  7. 7.
    Hamblin, D. L., Wood, A. W., Croft, R. J., & Stough, C. (2004). Examining the effects of electromagnetic fields emitted by GSM mobile phones on human event related potentials and performance during an auditory task. Clinical Neurophysiology, 115, 171.CrossRefGoogle Scholar
  8. 8.
    Krause, C. M., Pesonen, M., Haarala-Bjornberg, C., & Hamalainen. (2007). Effects of pulsed and continuous wave 902 MHz mobile phone exposure on brain oscillatory activity during cognitive processing. Bioelectromagnetics, 28, 296.CrossRefGoogle Scholar
  9. 9.
    Kumlin, T., Livonen, H., Miettenen, P., Junoven, A., van Groen, T., Puranen, L., et al. (2007). Mobile phone radiation and the developing brain: behavioral and morphological effects in juvenile rats. Radiation Research, 168, 471.CrossRefGoogle Scholar
  10. 10.
    Sievert, U., Eggert, S., & Pau, H. W. (2005). Can mobile phone emissions affect auditory functions of cochlea or brain stem? Otolaryngology and Head and Neck Surgery, 132, 451.CrossRefGoogle Scholar
  11. 11.
    Kumar, S., Kesari, K. K., & Behari, J. (2011). Synergistic effect of 2.45 GHz and pulsed magnetic field on reproductive pattern of male Wistar rats. Clinics, 66, 1–9. in press.CrossRefGoogle Scholar
  12. 12.
    Walliman, T., Dolder, M., Schlattner, U., Eder, M., Hornemann, T., Kraft, T., et al. (1998). Creatine kinase: an enzyme with a central role in cellular energy metabolism. Magma, 6, 116–119.CrossRefGoogle Scholar
  13. 13.
    Kessler, A., Costabeber, E., Dutra-Filho, C. S., Wyse, A. T. S., Wajner, M., & Wannmacher, C. M. D. (2003). Effect of proline on creatine kinase activity in rat brain. Metabolic Brain Disease, 18, 169–177.CrossRefGoogle Scholar
  14. 14.
    Saks, V. A., Ventura-Clapier, R., & Aliev, M. K. (1996). Metabolic control and metabolic capacity: Two aspects of creatine kinase functioning in the cells. Biochemistry Biophysics Acta, 1274, 81–88.CrossRefGoogle Scholar
  15. 15.
    Vaneeck, J. (1998). Cellular mechanisms of melatonin action. Physiological Reviews, 78, 687–721.Google Scholar
  16. 16.
    Adey, W. R. (1990). Electromagnetic field and the essence of living system. In J. B. Anderson (Ed.), Modern radio science (pp. 1–36). Oxford: Oxford University Press.Google Scholar
  17. 17.
    Paulraj, R., & Behari, J. (2002). The effect of low level continuous 2.45 GHz waves on enzymes of developing rat brain. Electromagnetic Biology and Medicine, 21, 233–243.CrossRefGoogle Scholar
  18. 18.
    Paulraj, R., Behari, J., & Rao, A. R. (1999). Effect of amplitude modulated RF radiation on calcium ion efflux and ODC activity in chronically exposed rat brain. Indian Journal of Biochemistry & Biophysics, 36, 337–340.Google Scholar
  19. 19.
    Kesari, K. K., & Behari, J. (2009). Fifty-gigahertz microwave exposure effect of radiations on rat brain. Applied Biochemistry and Biotechnology, 158, 126–139.CrossRefGoogle Scholar
  20. 20.
    Kesari, K. K., & Behari, J. (2010). Effect of microwave at 2.45 GHz radiations on reproductive system of male rats. Toxicological and Environmental Chemistry, 92, 1135–1147.CrossRefGoogle Scholar
  21. 21.
    Paulraj, R., & Behari, J. (2006). Single strand DNA breaks in rat brain cells exposed to microwave radiation. Mutation Research, 596, 76–80.CrossRefGoogle Scholar
  22. 22.
    Kesari, K. K., Kumar, S., & Behari, J. (2011). Effects of radiofrequency electromagnetic wave exposure from cellular phones on the reproductive pattern in male wistar rats. Applied Biochemistry and Biotechnology, 164, 546–559.CrossRefGoogle Scholar
  23. 23.
    Tomimoto, H., Yamamoto, K., Homburger, H. A., & Yanagihara, T. (1993). Immunoelectron microscopic investigation of creatine kinase BB-isoenzyme after cerebral ischemia in gerbils. Acta Neuropathology, 86, 447–455.Google Scholar
  24. 24.
    Manos, P., Bryan, G. K., & Edmond, J. (1991). Creatine kinase activity in postnatal rat brain development and in cultured neurons, astrocytes, and oligodendrocytes. Journal of Neurochemistry, 56, 2101–2107.CrossRefGoogle Scholar
  25. 25.
    Ceruti, S., Beltrami, E., Matarrese, P., Mazzola, A., Cattabeni, F., Malorni, W., et al. (2003). A key role for caspase-2 and caspase-3 in the apoptosis induced by 2-chloro-2′-deoxy-adenosine (cladribine) and 2-chloro-adenosine in human astrocytoma cells. Molecular Pharmacology, 63, 1437–1447.CrossRefGoogle Scholar
  26. 26.
    Riedl, S. J., & Shi, Y. (2004). Molecular mechanisms of caspase regulation during apoptosis. Nature Reviews Molecular Cell Biology, 5, 897–907.CrossRefGoogle Scholar
  27. 27.
    Pommier, Y., Sordet, O., Antony, S., Hayward, R. L., & Kohn, K. W. (2004). Apoptosis defects and chemotherapy resistance: molecular interaction maps and networks. Oncogene, 23, 2934–2949.CrossRefGoogle Scholar
  28. 28.
    Blank, M., & Shiloh, Y. (2007). Programs for cell death: Apoptosis is only one way to go. Cell Cycle, 6, 686.CrossRefGoogle Scholar
  29. 29.
    Kesari, K. K., Behari, J., & Kumar, S. (2010). Mutagenic response of 2.45GHz radiation exposure on rat brain. International Journal of Radiation Biology, 86, 334–343.CrossRefGoogle Scholar
  30. 30.
    Cayli, S., Sakkas, D., Vigue, L., Demir, R., & Huszar, G. (2004). Cellular maturity and apoptosis in human sperm: creatine kinase, caspase-3 and Bcl-XL levels in mature and diminished maturity sperm. Molecular Human Reproduction, 10, 365–372.CrossRefGoogle Scholar
  31. 31.
    IARC Monographs of the Evaluation of Carcinogenic Risks to Humans, volume 98. (2010): Painting, Frefighting and Shiftwork. Published by the International Agency for Research on Cancer, 150 cours Albert Thomas, 69372 Lyon Cedex 08, France. International Agency for Research on Cancer. ISBN 978 92 832 1298 0.Google Scholar
  32. 32.
    Henshaw, D. L., & Reiter, R. J. (2005). Do magnetic fields cause increased risk of childhood leukaemia via melatonin disruption? Bioelectromagnetics, 7, S86–S97.CrossRefGoogle Scholar
  33. 33.
    Wang, S. G. (1989). 5-HT contents change in peripheral blood of workers exposed to microwave and high frequency radiation. Chung Hua Yu Fang I Hsueh Tsa Chih, 23, 207–210.Google Scholar
  34. 34.
    Ebashi, W., Mikawa, T., Hirata, M., & Ad Nonomura, Y. (1978). The regulatory role of calcium in muscle. Annals of New York Academic Science, 307, 451.CrossRefGoogle Scholar
  35. 35.
    Ramussen, H., & Ad Waisman, D. M. (1981). The messenger function of calcium in endocrine systems. In G. Liwack (Ed.), “Biochemical actions of hormones” (Vol. 8, pp. 1–115). New York: Academic.Google Scholar
  36. 36.
    Commonwealth Scientific and Industrial Research Organization (CSIRO) Report. (1994). The cell membrane, ion exchange and cellular effects of EMR. In: Status of research on biological effects and safety of electromagnetic radiation telecommunications frequencies. June 1994.Google Scholar
  37. 37.
    Bawin, S., Adey, W., & Sabbot, I. (1978). Ionic factors in release of 45 Ca2+ from chicken cerebral tissues by electromagnetic fields. Proceedings of the National Academy of Sciences, 75, 6314–6318.CrossRefGoogle Scholar
  38. 38.
    Blackman, C. F., Benane, S. G., Elder, J. A., House, D. E., Lampe, J. A., & Faulk, J. M. (1980). Induction of calcium-ion efflux from brain tissue by radiofrequency radiation: effect of sample number and modulation frequency on the power-density window. Bioelectromagnetics, 1, 35–43.CrossRefGoogle Scholar
  39. 39.
    Rao, V. S., Titushkin, I. A., Moros, E. G., Pickard, W. F., Thatte, H. S., & Cho, M. R. (2008). Nonthermal effects of radiofrequency-field exposure on calcium dynamics in stem cell-derived neuronal cells: elucidation of calcium pathways. Radiation Research, 169, 319–329.CrossRefGoogle Scholar
  40. 40.
    Paulraj, R., & Behari, J. (2004). Radiofrequency radiation effect on protein kinase C activity in rats’ brain. Mutation Research, 585, 127–131.Google Scholar
  41. 41.
    Kesari, K. K., Kumar, S., & Behari, J. (2011). 900-MHz microwave radiation promotes oxidation in rat brain. Electromagnetic Biology and Medicine. doi: 10.3109/15368378.2011.587930.

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Kavindra Kumar Kesari
    • 1
  • Sanjay Kumar
    • 1
  • Jitendra Behari
    • 1
  1. 1.Bioelectromagnetic Laboratory, School of Environmental SciencesJawaharlal Nehru UniversityNew DelhiIndia

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