Applied Biochemistry and Biotechnology

, Volume 166, Issue 1, pp 13–21 | Cite as

Solvent-Free Production of Bioflavors by Enzymatic Esterification of Citronella (Cymbopogon winterianus) Essential Oil

  • Natália Paroul
  • Luana Paula Grzegozeski
  • Viviane Chiaradia
  • Helen Treichel
  • Rogério L. Cansian
  • J. Vladimir Oliveira
  • Débora de Oliveira


Enzymatic esterification of citronella essential oil towards the production of geranyl and citronellyl esters may present great scientific and technological interest due to the well-known drawbacks of the chemical-catalyzed route. In this context, this work reports the maximization of geranyl and citronellyl esters production by esterification of oleic and propionic acids in a solvent-free system using a commercial immobilized lipase as catalyst. Results of the reactions showed that the strategy adopted for the experimental design proved to be useful in evaluating the effects of the studied variables on the reaction conversion using Novozym 435 as catalyst. The operating conditions that maximized the production of each ester were determined, leading, in a general way, to conversions of about 90% for all systems. New experimental data on enzymatic esterification of crude citronella essential oil for geranyl and citronellyl esters production in solvent-free system are reported in this work.


Cymbopogon winterianus Essential oil Esterification Lipase 



The authors thank CNPq, CAPES, FAPERGS, and URI-Campus de Erechim for the financial support and scholarships.


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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Natália Paroul
    • 1
    • 2
  • Luana Paula Grzegozeski
    • 2
  • Viviane Chiaradia
    • 2
  • Helen Treichel
    • 2
  • Rogério L. Cansian
    • 2
  • J. Vladimir Oliveira
    • 2
  • Débora de Oliveira
    • 2
  1. 1.Programa de Pós-Graduação em BiotecnologiaUniversidade de Caxias do SulCaxias do SulBrazil
  2. 2.Universidade Regional Integrada do Alto Uruguai e das Missões, URI—Campus de ErechimErechimBrazil

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