Proteome Analysis of Abundant Proteins Extracted from the Leaf of Gynura procumbens (Lour.) Merr
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Gynura procumbens (Lour.) Merr. is a traditionally used medicinal plant to decrease cholesterol level, reduce high blood pressure, control diabetics, and for treatment of cancer. In our present study, a proteomic approach was applied to study the proteome of the plant that had never analyzed before. We have identified 92 abundantly expressed proteins from the leaves of G. procumbens (Lour.) Merr. Amongst the identified proteins was miraculin, a taste-masking agent with high commercial value. Miraculin made up ∼0.1% of the total protein extracted; the finding of miraculin gave a great commercial value to G. procumbens (Lour.) Merr. as miraculin’s natural source is limited while the production of recombinant miraculin faced challenges of not being able to exhibit the taste-masking effect as in the natural miraculin. We believe the discovery of miraculin in G. procumbens (Lour.) Merr., provides commercial feasibility of miraculin in view of the availability of G. procumbens (Lour.) Merr. that grow wildly and easily in tropical climate.
KeywordsGynura procumbens (Lour.) Merr Proteomics Leaf extract Protein profiling Miraculin
This work was supported by RU grant from Universiti Sains Malaysia (Project number: 1001/PFARMASI/815034). We thank the National Institute of Pharmaceutical and Nutraceutical, the Ministry of Science, Technology and Innovation, Malaysia for the use of the mass spectrometry instrument to analyze proteins, the Institute for Graduate Studies for providing scholarship to Hew Chaw-Sen.
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