Applied Biochemistry and Biotechnology

, Volume 165, Issue 3–4, pp 1024–1036 | Cite as

Relatively High-Substrate Consistency Hydrolysis of Steam-Pretreated Sweet Sorghum Bagasse at Relatively Low Cellulase Loading

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Abstract

Sweet sorghum bagasse (SSB) was steam pretreated in the conditions of 190 °C for 5 min to assess its amenability to the pretreatment and enzymatic hydrolysis. Results showed that pretreatment conditions were robust enough to pretreat SSB with maximum of 87% glucan and 72% xylan recovery. Subsequent enzymatic hydrolysis showed that the pretreated SSB at 2% substrate consistency resulted in maximum of 70% glucan–glucose conversion. Increasing substrate consistency from 2% to 16% led to a significant reduction in glucan conversion. However, the decrease ratio of glucan–glucose conversion was the minimum when the consistency increased from 2% to 12%. When the pretreated SSB consistency of 12% was applied for hydrolysis, increase in cellulase loading from 7.5 up to 20 filter paper units (FPU)/g glucan resulted only in 14% increase in glucan–glucose conversion compared to 20% increase with cellulase loading varying from 2.5 to 7.5 FPU/g glucan. More than 10 cellobiase units (CBU)/g glucan β-glucosidase supplementation had no noticeable improvement on glucan–glucose conversion. Additionally, supplementation of xylanase was found to significantly increase glucan–glucose conversion from 50% to 80% with the substrate consistency of 12%, when the cellulase and β-glucosidase loadings were at relatively low enzyme loadings (7.5 FPU/g and 10 CBU/g glucan). It appeared that residual xylan played a critical role in hindering the ease of hydrolysis of SSB. A proper xylanase addition was suggested to achieve a high hydrolysis yield at relatively high substrate consistency with relatively low enzyme loadings.

Keywords

Sweet sorghum bagasse Steam pretreatment Relatively high substrate consistency Enzymatic hydrolysis Xylanase addition 
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Notes

Acknowledgments

The first author gives appreciation to the China Scholarship Council of the Ministry of Education of China for the financial support to study in the University of British of Columbia. The authors are also thankful to Mr. Linoj Kumar for all technical and editorial help while preparing this manuscript. The authors also thank Dr. Sonia Ghatora for the help with the steam pretreatment and Mr. Pablo A Chung for the technical support provided with HPLC analysis.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.College of Resource and EnvironmentSichuan Agricultural University, Chengdu CampusChengduChina
  2. 2.Forest Products Biotechnology, Department of Wood ScienceUniversity of British ColumbiaVancouverCanada
  3. 3.College of Chemical EngineeringSichuan UniversityChengduChina
  4. 4.Biomass Energy Research CentreShanghai Jiao Tong UniversityShanghaiChina

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