Applied Biochemistry and Biotechnology

, Volume 165, Issue 3–4, pp 963–977 | Cite as

Isolation, Identification, and Culture Optimization of a Novel Glycinonitrile-Hydrolyzing Fungus—Fusarium oxysporum H3

  • Jin-Song Gong
  • Zhen-Ming Lu
  • Jing-Song Shi
  • Wen-Fang Dou
  • Hong-Yu Xu
  • Zhe-Min Zhou
  • Zheng-Hong Xu
Article
First Online:
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Accepted:

Abstract

Microbial transformation of glycinonitrile into glycine by nitrile hydrolase is of considerable interest to green chemistry. A novel fungus with high nitrile hydrolase was newly isolated from soil samples and identified as Fusarium oxysporum H3 through 18S ribosomal DNA, 28S ribosomal DNA, and the internal transcribed spacer sequence analysis, together with morphology characteristics. After primary optimization of culture conditions including pH, temperature, carbon/nitrogen sources, inducers, and metal ions, the enzyme activity was greatly increased from 326 to 4,313 U/L. The preferred carbon/nitrogen sources, inducer, and metal ions were glucose and yeast extract, caprolactam, and Cu2+, Mn2+, and Fe2+, respectively. The maximum enzyme formation was obtained when F. oxysporum H3 was cultivated at 30 °C for 54 h with the initial pH of 7.2. There is scanty report about the optimization of nitrile hydrolase production from nitrile-converting fungus.

Keywords

Fusarium oxysporum Glycinonitrile Identification Nitrile hydrolase Optimization 
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Notes

Acknowledgments

The authors would sincerely like to thank Prof. Zhi-Song Shen, whose gracious support made this research possible. Also, financial supports from the program for New Century Excellent Talents in University of China (No. NCET-07-0380), the Major Basic Research Development Program of China (No. 2007CB707804), and the National High Technology Research and Development Program of China (No. 2006AA020104) are gratefully acknowledged.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Jin-Song Gong
    • 1
  • Zhen-Ming Lu
    • 2
  • Jing-Song Shi
    • 3
  • Wen-Fang Dou
    • 2
  • Hong-Yu Xu
    • 2
  • Zhe-Min Zhou
    • 1
  • Zheng-Hong Xu
    • 1
    • 2
  1. 1.The Key Laboratory of Industrial Biotechnology, Ministry of EducationJiangnan UniversityWuxiPeople’s Republic of China
  2. 2.Laboratory of Pharmaceutical Engineering, School of Medicine and PharmaceuticsJiangnan UniversityWuxiPeople’s Republic of China
  3. 3.Laboratory of Bioactive Products Processing Engineering, School of Medicine and PharmaceuticsJiangnan UniversityWuxiPeople’s Republic of China

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