Applied Biochemistry and Biotechnology

, Volume 165, Issue 2, pp 442–453 | Cite as

Lipid Production by Culturing Oleaginous Yeast and Algae with Food Waste and Municipal Wastewater in an Integrated Process

  • Zhanyou Chi
  • Yubin Zheng
  • Anping Jiang
  • Shulin Chen


Food waste and municipal wastewater are promising feedstocks for microbial lipid biofuel production, and corresponding production process is to be developed. In this study, different oleaginous yeast strains were tested to grow in hydrolyzed food waste, and growths of Cryptococcus curvatus, Yarrowia lipolytica, and Rhodotorula glutinis in this condition were at same level as in glucose culture as control. These strains were further tested to grow in municipal primary wastewater. C. curvatus and R. glutinis had higher production than Y. lipolytica in media made from primary wastewater, both with and without glucose supplemented. Finally, a process was tested to grow C. curvatus and R. glutinis in media made from food waste and municipal wastewater, and the effluents from these processes were further treated with yeast culture and phototrophic algae culture; 1.1 g/L C. curvatus and 1.5 g/L R. glutinis biomass were further produced in second-step yeast cultures, as well as 1.53 and 0.58 g/L Chlorella sorokiniana biomass in phototrophic cultures. The residual nitrogen concentrations in final effluents were 33 mg/L and 34 mg/L, respectively, and the residual phosphorus concentrations were 1.5 and 0.6 mg/L, respectively. The lipid contents in the produced biomass were from 18.7% to 28.6%.


Oleaginous yeast Algae Biodiesel Municipal wastewater Food waste 


  1. 1.
    Moreton, R. S. (1988). Single cell oil. New York: Wiley.Google Scholar
  2. 2.
    Kyle, D., & Ratledge, C. (1992). Industrial applications of single cell oils. Champaign: American Oil Chemists’ Society.CrossRefGoogle Scholar
  3. 3.
    Ward, O. P., & Singh, A. (2005). Omega-3/6 fatty acids: alternative sources of production. Process Biochemistry, 40(12), 3627–3652.CrossRefGoogle Scholar
  4. 4.
    Bailey RB, DiMasi D, Hansen JM, Mirrasoul PJ, Ruecker CM, Veeder GT III et al. (2003) Enhanced production of lipids containing polyenoic fatty acid by very high density cultures of eukaryotic microbes in fermentors. US Patent 6607900.Google Scholar
  5. 5.
    Xiong, W., Li, X. F., Xiang, J. Y., & Wu, Q. Y. (2008). High-density fermentation of microalga Chlorella protothecoides in bioreactor for microbio-diesel production. Applied Microbiology and Biotechnology, 78, 29–36.CrossRefGoogle Scholar
  6. 6.
    Hu, C. M., Zhao, X., Zhao, J., Wu, S. G., & Zhao, Z. B. K. (2009). Effects of biomass hydrolysis by-products on oleaginous yeast Rhodosporidium toruloides. Bioresource Technology, 100(20), 4843–4847.CrossRefGoogle Scholar
  7. 7.
    Xue, F. Y., Miao, J. X., Zhang, X., Luo, H., & Tan, T. W. (2008). Studies on lipid production by Rhodotorula glutinis fermentation using monosodium glutamate wastewater as culture medium. Bioresource Technology, 99(13), 5923–5927.CrossRefGoogle Scholar
  8. 8.
    Xue, F. Y., Zhang, X., Luo, H., & Tan, T. W. (2006). A new method for preparing raw material for biodiesel production. Process Biochemistry, 41(7), 1699–1702.CrossRefGoogle Scholar
  9. 9.
    Kantor, L. S., Lipton, K., Manchester, A., & Oliveira, V. (1997). Estimating and addressing America’s food losses. Food Review, 20(1), 2–12.Google Scholar
  10. 10.
    Frear C, Zhao B, Fu G, Richardson M, Chen S. (2005) Biomass inventory and bioenergy assessment. Report from Washington State Department of Ecology.Google Scholar
  11. 11.
    Zhang, R. H., El-Mashad, H. M., Hartman, K., Wang, F. Y., Liu, G. Q., Choate, C., et al. (2007). Characterization of food waste as feedstock for anaerobic digestion. Bioresource Technology, 98(4), 929–935.CrossRefGoogle Scholar
  12. 12.
    Davis, R. A. (2008). Parameter estimation for simultaneous saccharification and fermentation of food waste into ethanol using Matlab Simulink. Applied Biochemistry and Biotechnology, 147(1–3), 11–21.CrossRefGoogle Scholar
  13. 13.
    GLUMRB. (2004). Recommended standards for wastewater facilities (10-States’ Standard). Albany: Health Research.Google Scholar
  14. 14.
    Metcalf, E. I. (2003). Wastewater engineering treatment and reuse. New York: McGraw-Hill.Google Scholar
  15. 15.
    Mondala, A., Liang, K. W., Toghiani, H., Hernandez, R., & French, T. (2009). Biodiesel production by in situ transesterification of municipal primary and secondary sludges. Bioresource Technology, 100(3), 1203–1210.CrossRefGoogle Scholar
  16. 16.
    Chen, F., & Johns, M. R. (1991). Effect of carbon to nitrogen ratio and aeration on the fatty acid composition of heterotrophic Chlorella sorokiniana. Journal of Applied Phycology, 3(3), 203–210.CrossRefGoogle Scholar
  17. 17.
    Chi, Z. Y., Pyle, D., Wen, Z. Y., Frear, C., & Chen, S. L. (2007). A laboratory study of producing docosahexaenoic acid from biodiesel-waste glycerol by microalgal fermentation. Process Biochemistry, 42, 1537–1545.CrossRefGoogle Scholar
  18. 18.
    Pirt, J. (1975). Principles of microbe and cell cultivation. New York: Wiley.Google Scholar
  19. 19.
    Meesters, P., Huijberts, G. N. M., & Eggink, G. (1996). High cell density cultivation of the lipid accumulating yeast Cryptococcus curvatus using glycerol as a carbon source. Applied Microbiology and Biotechnology, 45(5), 575–579.CrossRefGoogle Scholar
  20. 20.
    Evans, C. T., & Ratledge, C. (1983). A comparison of the oleaginous yeast, Candida curvata, grown on different carbon sources in continuous and batch culture. Lipids, 18(9), 623–629.CrossRefGoogle Scholar
  21. 21.
    Daniel, H. J., Otto, R. T., Binder, M., Reuss, M., & Syldatk, C. (1999). Production of sophorolipids from whey: development of a two-stage process with Cryptococcus curvatus ATCC 20509 and Candida bombicola ATCC 22214 using deproteinized whey concentrates as substrates. Applied Microbiology and Biotechnology, 51(1), 40–45.CrossRefGoogle Scholar
  22. 22.
    Akindumila, F., & Glatz, B. A. (1998). Growth and oil production of Apiotrichum curvatum in tomato juice. Journal of Food Protection, 61(11), 1515–1517.Google Scholar
  23. 23.
    Papanikolaou, S., & Aggelis, G. (2002). Lipid production by Yarrowia lipolytica growing on industrial glycerol in a single-stage continuous culture. Bioresource Technology, 82(1), 43–49.CrossRefGoogle Scholar
  24. 24.
    Papanikolaou, S., Chevalot, I., Komaitis, M., Aggelis, G., & Marc, I. (2001). Kinetic profile of the cellular lipid composition in an oleaginous Yarrowia lipolytica capable of producing a cocoa-butter substitute from industrial fats. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 80(3–4), 215–224.CrossRefGoogle Scholar
  25. 25.
    Li, Y. H., Zhao, Z. B., & Bai, F. W. (2007). High-density cultivation of oleaginous yeast Rhodosporidium toruloides Y4 in fed-batch culture. Enzyme and Microbial Technology, 41(3), 312–317.CrossRefGoogle Scholar
  26. 26.
    Angerbauer, C., Siebenhofer, M., Mittelbach, M., & Guebitz, G. M. (2008). Conversion of sewage sludge into lipids by Lipomyces starkeyi for biodiesel production. Bioresource Technology, 99(8), 3051–3056.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Zhanyou Chi
    • 1
  • Yubin Zheng
    • 1
  • Anping Jiang
    • 1
  • Shulin Chen
    • 1
  1. 1.Department of Biological Systems EngineeringWashington State UniversityPullmanUSA

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