Applied Biochemistry and Biotechnology

, Volume 164, Issue 5, pp 561–572 | Cite as

Comparison of the Properties of Lipase Immobilized onto Mesoporous Resins by Different Methods

Article
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Abstract

Genipin, a natural cross-linking agent, was used for the immobilization of lipase from Candida sp. 99-125 by cross-linking to two kinds of mesoporous resins. Under optimum conditions, the activity recovery of immobilized lipase on resin NKA-9 could reach up to 96.99% when the genipin concentration was 0.5%, and it could reach up to 86.18% for S-8 with a genipin concentration of 0.25%. Compared with using glutaraldehyde as a cross-linking agent, the immobilized lipase using genipin showed better pH and thermal stability, storage stability, and reusability. The residual activity of immobilized lipase using genipin as cross-linker remained more than 60% of its initial activity after six hydrolytic cycles, whereas only about 35% activity remained by using glutaraldehyde as cross-linker.

Keywords

Immobilized lipase Cross-linking Genipin Glutaraldehyde Stability 
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Notes

Acknowledgments

This work was supported by the Natural Science Foundation of Hebei Province (B2010000035, B2008000028) and the Natural Science Foundation of Tianjin (08JCYBJC02400).

Supplementary material

12010_2010_9157_MOESM1_ESM.doc (797 kb)
ESM 1 (DOC 797 kb)

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Wenqin Wang
    • 1
  • Yanjun Jiang
    • 1
  • Liya Zhou
    • 1
  • Jing Gao
    • 1
  1. 1.Department of Bioengineering, School of Chemical EngineeringHebei University of TechnologyTianjinChina

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