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Applied Biochemistry and Biotechnology

, Volume 164, Issue 4, pp 464–474 | Cite as

Nitrate as an Oxidant in the Cathode Chamber of a Microbial Fuel Cell for Both Power Generation and Nutrient Removal Purposes

  • Cheng Fang
  • Booki Min
  • Irini Angelidaki
Article

Abstract

Nitrate ions were used as the oxidant in the cathode chamber of a microbial fuel cell (MFC) to generate electricity from organic compounds with simultaneous nitrate removal. The MFC using nitrate as oxidant could generate a voltage of 111 mV (1,000 Ω) with a plain carbon cathode. The maximum power density achieved was 7.2 mW m−2 with a 470 Ω resistor. Nitrate was reduced from an initial concentration of 49 to 25 mg (NO 3 −N) L−1 during 42-day operation. The daily removal rate was 0.57 mg (NO 3 –N) L−1 day−1 with a voltage generation of 96 mV. In the presence of Pt catalyst dispersed on cathode, the cell voltage was significantly increased up to 450 mV and the power density was 117.7 mW m−2, which was 16 times higher than the value without Pt catalyst. Significant nitrate removal was also observed with a daily removal rate of 2 mg (NO 3 –N) L−1 day−1, which was 3.5 times higher compared with the operation without catalyst. Nitrate was reduced to nitrite and ammonia in the liquid phase at a ratio of 0.6% and 51.8% of the total nitrate amount. These results suggest that nitrate can be successfully used as an oxidant for power generation without aeration and also nitrate removal from water in MFC. However, control of the process would be needed to reduce nitrate to only nitrogen gas, and avoid further reduction to ammonia.

Keywords

Nitrate removal Microbial fuel cell Power generation Cathode chamber Electron acceptor 

Notes

Acknowledgment

The authors thank Óscar Benito Román; and also thank Hector Garcia for his help with analytical measurements. This research was supported by Danish Agency for Science Technology and Innovation, 2104-05-0003. This work was also supported by the Ph.D. scholarship from the Department of Environmental Engineering, Technical University of Denmark.

References

  1. 1.
    Hallberg, G. R. (1989). Nitrate in ground water in the United States. In R. F. Follet (Ed.), Nitrogen management and ground water protection (pp. 35–74). Amsterdam: Elsevier.Google Scholar
  2. 2.
    Puckett LJ (1995). Identifying the major sources of nutrient water pollution. Environmental Science & Technology, 29(9), 408A–414A.Google Scholar
  3. 3.
    Freshwater in Europe-Facts, Figures and Maps. Division of Early Warning and Assessment, Office for Europe (DEWA ∼ Europe), United Nations Environment Programme (UNEP). Available from: http://www.grid.unep.ch/product/publication/freshwater_europe.php. Accessed January 05, 2010.
  4. 4.
    Virdis, B., Rabaey, K., Rozendal, R. A., Yuan, Z., & Keller, J. (2010). Simultaneous nitrification, denitrification and carbon removal in microbial fuel cells. Water Res., 44(9), 2970–2980.CrossRefGoogle Scholar
  5. 5.
    Canter, L. W. (1997). Nitrates in groundwater. Boca Raton: CRC Press.Google Scholar
  6. 6.
    Haugen, K. S., Semmens, M. J., & Novak, P. J. (2002). A novel in situ technology for the treatment of nitrate contaminated groundwater. Water Res., 36, 3497–3506.CrossRefGoogle Scholar
  7. 7.
    Till, B. A., Weathers, L. J., & Alvarez, P. J. J. (1998). Fe(0)-supported autotrophic denitrification. Environ. Sci. Technol., 32, 634–639.CrossRefGoogle Scholar
  8. 8.
    Oh, S. E., & Logan, B. E. (2006). Proton exchange membrane and electrode surface areas as factors that affect power generation in microbial fuel cells. Appl. Microbiol. Biotechnol., 70, 162–169.CrossRefGoogle Scholar
  9. 9.
    NLM, RTECS (Registry of Toxic Effects of Chemical Substances), Bethesda, MD, Record No. 36474, 1999.Google Scholar
  10. 10.
    Oh, S. E., Min, B., & Logan, B. E. (2004). Cathode performance as a factor in electricity generation in microbial fuel cells. Environ. Sci. Technol., 38, 4900–4904.CrossRefGoogle Scholar
  11. 11.
    Jia, Y. H., Tran, H. T., Kim, D. H., Oh, S. J., Park, D. H., Zhang, R. H., et al. (2008). Simultaneous organics removal and bio-electrochemical denitrification in microbial fuel cells. Bioprocess Biosyst. Eng., 31, 315–321.CrossRefGoogle Scholar
  12. 12.
    Lefebvre, O., Al-Mamun, A., & Ng, H. Y. (2008). A microbial fuel cell equipped with a biocathode for organic removal and denitrification. Water Sci. Technol., 58(4), 881–885.CrossRefGoogle Scholar
  13. 13.
    Koroleva, O. V., Yavmetdinov, I. S., Shleev, S. V., Stepanova, E. V., & Gavrilova, V. P. (2001). Isolation and study of some properties of laccase from the basidiomycetes Cerrena maxima. Biochemistry (Moscow), 66(6), 618–622.CrossRefGoogle Scholar
  14. 14.
    Liu, H., Ramnarayanan, R., & Logan, B. E. (2004). Production of electricity during wastewater treatment using a single chamber microbial fuel cell. Environ. Sci. Technol., 38, 2281–2285.CrossRefGoogle Scholar
  15. 15.
    Cheng, S., Liu, H., & Logan, B. E. (2006). Increased performance of single chamber microbial fuel cells using an improved cathode structure. Electrochem. Commun., 8(3), 489–494.CrossRefGoogle Scholar
  16. 16.
    Cheng, S., Liu, H., & Logan, B. E. (2006). Power densities using different cathode catalysts (Pt and CoTMPP) and polymer binders (Nafion and PTFE) in single chamber microbial fuel cells. Environ. Sci. Technol., 40(1), 364–369.CrossRefGoogle Scholar
  17. 17.
    HaoYu, E., Cheng, S., Scott, K., & Logan, B. E. (2007). Microbial fuel cell performance with non-Pt cathode catalysts. J. Power Sources, 171, 275–281.CrossRefGoogle Scholar
  18. 18.
    American Public Health Association, American Water Works Association, Water Pollution Control Federation. (1995). Standard methods for the examination of water and wastewater (19th ed.). Washington, DC: American Public Health Association.Google Scholar
  19. 19.
    EPA method 353.2, nitrogen, nitrate-nitrite (colorimetric, automated, cadmium reduction)Google Scholar
  20. 20.
    Liu, H., Cheng, S., & Logan, B. E. (2005). Production of electricity from acetate or butyrate using a single-chamber microbial fuel cell. Environ. Sci. Technol., 39, 658–662.CrossRefGoogle Scholar
  21. 21.
    Clauwaert, P., Ha, D. V. D., Boon, N., Verbeken, K., Verhaege, M., Rabaey, K., et al. (2007). Open air biocathode enables effective electricity generation with microbial fuel cells. Environ. Sci. Technol., 41, 7564–7569.CrossRefGoogle Scholar
  22. 22.
    Virdis, B., Rabaey, K., Yuan, Z., & Keller, J. (2008). Microbial fuel cells for simultaneous carbon and nitrogen removal. Water Res., 42, 3013–3024.CrossRefGoogle Scholar
  23. 23.
    Min, B., Roman, O. B., & Angelidaki, I. (2008). Importance of temperature and anodic medium composition on microbial fuel cell (MFC) performance. Biotechnol. Lett., 30, 1213–1218.CrossRefGoogle Scholar
  24. 24.
    Park, H. I., Kim, D. K., Choi, Y. J., & Park, D. (2005). Nitrate reduction using an electrode as direct electron donor in a biofilm-electrode reactor. Process Biochem., 40, 3383–3388.CrossRefGoogle Scholar
  25. 25.
    Cha, J., Kim, C., Choi, S., Lee, G., Chen, G., & Lee, T. (2009). Evaluation of microbial fuel cell coupled with aeration chamber and bio-cathode for organic matter and nitrogen removal from synthetic domestic wastewater. Water Sci. Technol., 60(6), 1409–1418.CrossRefGoogle Scholar
  26. 26.
    Liu, H., & Logan, B. E. (2004). Electricity generation using an air-cathode single chamber microbial fuel cell in the presence and absence of a proton exchange membrane. Environ. Sci. Technol., 38, 4040–4046.CrossRefGoogle Scholar
  27. 27.
    Min, B., Cheng, S. A., & Logan, B. E. (2005). Electricity generation using membrane and salt bridge microbial fuel cells. Water Res., 39, 1675–1686.CrossRefGoogle Scholar
  28. 28.
    Virdis, B., Rabaey, K., Yuan, Z., Rozendal, R. A., & Keller, J. (2009). Electron fluxes in a microbial fuel cell performing carbon and nitrogen removal. Environ. Sci. Technol., 43, 5144–5149.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of Environmental EngineeringTechnical University of DenmarkLyngbyDenmark
  2. 2.Department of Environmental Science and EngineeringKyung Hee UniversityYongin-siSouth Korea

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