Applied Biochemistry and Biotechnology

, Volume 164, Issue 3, pp 256–267

Cellulase Production by Streptomyces viridobrunneus SCPE-09 Using Lignocellulosic Biomass as Inducer Substrate

  • Fábio Nuno Marques Da Vinha
  • Mônica Pires Gravina-Oliveira
  • Marcella Novaes Franco
  • Andrew Macrae
  • Elba Pinto da Silva Bon
  • Rodrigo Pires Nascimento
  • Rosalie Reed Rodrigues Coelho
Article

DOI: 10.1007/s12010-010-9132-8

Cite this article as:
Da Vinha, F.N.M., Gravina-Oliveira, M.P., Franco, M.N. et al. Appl Biochem Biotechnol (2011) 164: 256. doi:10.1007/s12010-010-9132-8

Abstract

An actinomycete strain, isolated from a soil sample under a sugar cane plantation in Brazil and identified as Streptomyces viridobrunneus SCPE-09, was selected as a promising cellulolytic strain, and tested for its ability to produce cellulases from agro-industrial residues. Sugar cane bagasse or wheat bran was tested as carbon source, and corn steep liquor tested as nitrogen source. Different concentrations of carbon and nitrogen were tested using factorial design to identify optimal cellulose production. The results showed that media containing wheat bran 2.0% (w/v) and corn steep liquid 0.19% (w/v) lead to the highest production, 2.0 U mL−1 of CMCase, obtained on the fifth day of fermentation. The pH and temperature profile showed optimal activity at pH 4.9 and 50°C. As for thermostability, endoglucanases were most tolerant at 50°C, retaining more than 80% of maximal activity even after 2 h of incubation. Zymogram analyses using supernatant from growth under optimized conditions revealed the presence of two CMCase bands with apparent molecular masses of 37 and 119 kDa. The combination of pH tolerance and CMCase production from agro-industrial residues by S. viridobrunneus SCPE-09 offers promise for future bioethanol biotechnologies.

Keywords

Streptomyces viridobrunneus CMCase Corn steep liquor Sugar cane bagasse Wheat bran 

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Fábio Nuno Marques Da Vinha
    • 1
  • Mônica Pires Gravina-Oliveira
    • 1
  • Marcella Novaes Franco
    • 1
  • Andrew Macrae
    • 1
  • Elba Pinto da Silva Bon
    • 3
  • Rodrigo Pires Nascimento
    • 2
  • Rosalie Reed Rodrigues Coelho
    • 1
  1. 1.Dep. Microbiologia Geral, Instituto de Microbiologia Prof. Paulo de Góes, CCSUniversidade Federal do Rio de Janeiro (UFRJ)Rio de JaneiroBrazil
  2. 2.Centro de Ciências Agrárias, Ambientais e BiológicasUniversidade Federal do Recôncavo da Bahia (UFRB)Cruz das AlmasBABrazil
  3. 3.Instituto de Química, CTUniversidade Federal do Rio de Janeiro (UFRJ)Rio de JaneiroBrazil

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