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Applied Biochemistry and Biotechnology

, Volume 163, Issue 7, pp 928–936 | Cite as

Ethanol Production from Residual Wood Chips of Cellulose Industry: Acid Pretreatment Investigation, Hemicellulosic Hydrolysate Fermentation, and Remaining Solid Fraction Fermentation by SSF Process

  • Neumara Luci Conceição Silva
  • Gabriel Jaime Vargas Betancur
  • Mariana Peñuela Vasquez
  • Edelvio de Barros Gomes
  • Nei PereiraJr.
Article

Abstract

Current research indicates the ethanol fuel production from lignocellulosic materials, such as residual wood chips from the cellulose industry, as new emerging technology. This work aimed at evaluating the ethanol production from hemicellulose of eucalyptus chips by diluted acid pretreatment and the subsequent fermentation of the generated hydrolysate by a flocculating strain of Pichia stipitis. The remaining solid fraction generated after pretreatment was subjected to enzymatic hydrolysis, which was carried out simultaneously with glucose fermentation [saccharification and fermentation (SSF) process] using a strain of Saccharomyces cerevisiae. The acid pretreatment was evaluated using a central composite design for sulfuric acid concentration (1.0–4.0 v/v) and solid to liquid ratio (1:2–1:4, grams to milliliter) as independent variables. A maximum xylose concentration of 50 g/L was obtained in the hemicellulosic hydrolysate. The fermentation of hemicellulosic hydrolysate and the SSF process were performed in bioreactors and the final ethanol concentrations of 15.3 g/L and 28.7 g/L were obtained, respectively.

Keywords

Residual wood chips Acid pretreatment Hemicellulose Cellulose Bioethanol 

Notes

Acknowledgments

The authors are deeply grateful to the Brazilian Council for Research (CNPq), the Rio de Janeiro State Foundation for Science & Technology (FAPERJ), and the Brazilian Oil Company (PETROBRAS) for grants and other financial supports.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Neumara Luci Conceição Silva
    • 1
  • Gabriel Jaime Vargas Betancur
    • 1
  • Mariana Peñuela Vasquez
    • 2
  • Edelvio de Barros Gomes
    • 1
  • Nei PereiraJr.
    • 1
  1. 1.Biochemical Engineering DepartmentFederal University of Rio de JaneiroRio de JaneiroBrazil
  2. 2.Engineering Faculty–Chemical Engineering DepartmentUniversity of AntioquiaMedellínColombia

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