Applied Biochemistry and Biotechnology

, Volume 163, Issue 7, pp 845–850 | Cite as

Applications of Loop-Mediated Isothermal DNA Amplification

  • Shijun FuEmail author
  • Guanggang Qu
  • Shijin Guo
  • Lin Ma
  • Na Zhang
  • Songlin Zhang
  • Sanyang Gao
  • Zhiqiang Shen


During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. Expensive equipment are not necessary to acquire a high level of precision, and there are fewer preparation steps compared to conventional PCR and real-time PCR assays. This paper briefly summarized the applications of LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification.


Loop-mediated isothermal amplification Pathogenic microorganisms Detection Application 



The authors gratefully acknowledge Miss Shirley Wong for the critical reading of the manuscript. This study was financially supported by the Shandong Science and Technology Development Project of People’s Republic of China (2009GG10009001).


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Shijun Fu
    • 1
    • 2
    Email author
  • Guanggang Qu
    • 1
    • 3
  • Shijin Guo
    • 1
    • 2
  • Lin Ma
    • 2
  • Na Zhang
    • 3
  • Songlin Zhang
    • 1
  • Sanyang Gao
    • 1
    • 3
  • Zhiqiang Shen
    • 1
    • 2
    • 3
  1. 1.Key Laboratory of Preventive Veterinary Medicine and Animal BiotechnologyBinzhou Animal Husbandry and Veterinary Research AcademyBinzhouPeople’s Republic of China
  2. 2.Shandong Lvdu Ante Veterinary Drug Industry Co., LtdBinzhouPeople’s Republic of China
  3. 3.Shandong Lvdu Biological Technology Co., LtdBinzhouPeople’s Republic of China

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