Applied Biochemistry and Biotechnology

, Volume 163, Issue 7, pp 845–850 | Cite as

Applications of Loop-Mediated Isothermal DNA Amplification

  • Shijun Fu
  • Guanggang Qu
  • Shijin Guo
  • Lin Ma
  • Na Zhang
  • Songlin Zhang
  • Sanyang Gao
  • Zhiqiang Shen
Article

Abstract

During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. Expensive equipment are not necessary to acquire a high level of precision, and there are fewer preparation steps compared to conventional PCR and real-time PCR assays. This paper briefly summarized the applications of LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification.

Keywords

Loop-mediated isothermal amplification Pathogenic microorganisms Detection Application 

Notes

Acknowledgments

The authors gratefully acknowledge Miss Shirley Wong for the critical reading of the manuscript. This study was financially supported by the Shandong Science and Technology Development Project of People’s Republic of China (2009GG10009001).

References

  1. 1.
    Notomi, T., Okayama, H., & Masubuchi, H. (2000). Nucleic Acids Research, 28, e63.CrossRefGoogle Scholar
  2. 2.
    Ushikubo, H. (2004). Uirusu, 54, 107–112.CrossRefGoogle Scholar
  3. 3.
    Hara-Kudo, Y., Yoshino, M., Kojima, T., & Ikedo, M. (2005). FEMS Microbiology Letters, 253, 155–161.CrossRefGoogle Scholar
  4. 4.
    Hara-Kudo, Y., Nemoto, J., Ohtsuka, K., Segawa, Y., Takatori, K., Kojima, T., et al. (2007). Journal of Medical Microbiology, 56, 398–406.CrossRefGoogle Scholar
  5. 5.
    Yamazaki, W., Taguchi, M., Ishibashi, M., Kitazato, M., Nukina, M., Misawa, N., et al. (2008). Journal of Medical Microbiology, 57, 444–451.CrossRefGoogle Scholar
  6. 6.
    Yamazaki, W., Taguchi, M., Kawai, T., Kawatsu, K., Sakata, J., Inoue, K., et al. (2009). Applied and Environmental Microbiology, 75, 1597–1603.CrossRefGoogle Scholar
  7. 7.
    Maruyama, F., Kenzaka, T., Yamaguchi, N., Tani, K., & Nasu, M. (2003). Applied and Environmental Microbiology, 69, 5023–5028.CrossRefGoogle Scholar
  8. 8.
    Osawa, R., Yoshida, A., Masakiyo, Y., Nagashima, S., Ansai, T., Watari, H., et al. (2007). Oral Microbiology and Immunology, 22, 252–259.CrossRefGoogle Scholar
  9. 9.
    Iwamoto, T., Sonobe, T., & Hayashi, K. (2003). Journal of Clinical Microbiology, 41, 2616–2622.CrossRefGoogle Scholar
  10. 10.
    Seki, M., Yamashita, Y., Torigoe, H., Tsuda, H., Sato, S., & Maeno, M. (2005). Journal of Clinical Microbiology, 43, 1581–1586.CrossRefGoogle Scholar
  11. 11.
    Savan, R., Igarashi, A., Matsuoka, S., & Sakai, M. (2004). Applied and Environmental Microbiology, 70, 621–624.CrossRefGoogle Scholar
  12. 12.
    Yeh, H. Y., Shoesnaker, C. A., & Klesiue, P. H. (2005). Journal of Microbiological Methods, 63, 36–44.CrossRefGoogle Scholar
  13. 13.
    Misawa, Y., Yoshida, A., Saito, R., Yoshida, H., Okuzumi, K., Ito, N., et al. (2007). Journal of Infection and Chemotherapy, 13, 134–140.CrossRefGoogle Scholar
  14. 14.
    Qiao, Y. M., Guo, Y. C., Zhang, X. E., Zhou, Y. F., Zhang, Z. P., Wei, H. P., et al. (2007). Biotechnology Letters, 29, 1939–1946.CrossRefGoogle Scholar
  15. 15.
    Savan, R., Kono, T., Itami, T., & Sakai, M. (2005). Journal of Fish Diseases, 28, 573–581.CrossRefGoogle Scholar
  16. 16.
    Fukuta, S., Kato, S., Yoshida, K., Mizukami, Y., Ishida, A., Ueda, J., et al. (2003). Journal of Virological Methods, 12, 35–40.CrossRefGoogle Scholar
  17. 17.
    Qu, G. G., Fu, S. J., Shen, N., Wang, J. L., Xiao, Y. Q., Guan, Y., et al. (2010). Journal of Applied Animal Research, 37, 113–116.Google Scholar
  18. 18.
    Ihira, M., Akimoto, S., Miyake, F., Fujita, A., Sugata, K., Suga, S., et al. (2007). Journal of Clinical Virology, 39, 22–26.CrossRefGoogle Scholar
  19. 19.
    Ihira, M., Yoshikawa, T., Enomoto, Y., Akimoto, S., Ohashi, M., Suga, S., et al. (2004). Journal of Clinical Microbiology, 42, 140–145.CrossRefGoogle Scholar
  20. 20.
    Yoshikawa, T., Ihira, M., & Akimoto, S. (2004). Journal of Clinical Microbiology, 42, 1348–1352.CrossRefGoogle Scholar
  21. 21.
    Kaneko, H., Iida, T., Aoki, K., Ohno, S., & Suzutani, T. (2005). Journal of Clinical Microbiology, 43, 3290–3296.CrossRefGoogle Scholar
  22. 22.
    Poon, L. L., Leung, C. S., Chan, K. H., Lee, J. H., Yuen, K. Y., Guan, Y., et al. (2005). Journal of Clinical Microbiology, 43, 427–430.CrossRefGoogle Scholar
  23. 23.
    Chen, Q., Li, J., Fang, X. E., & Xiong, W. (2009). Intervirology, 52, 86–91.CrossRefGoogle Scholar
  24. 24.
    Fukuta, S., Iida, T., Mizukami, Y., Ishida, A., Ueda, J., Kanbe, M., et al. (2003). Archives of Virology, 148, 1713–1720.CrossRefGoogle Scholar
  25. 25.
    Imai, M., Ninomiya, A., Minekawa, H., Notomi, T., Ishizaki, T., Tashiro, M., et al. (2006). Vaccine, 24, 6679–6682.CrossRefGoogle Scholar
  26. 26.
    Imai, M., Ninomiya, A., Minekawa, H., Notomi, T., Ishizaki, T., Van-Tu, P., et al. (2007). Journal of Virological Methods, 141, 173–180.CrossRefGoogle Scholar
  27. 27.
    Kiatpathomchai, W., Jareonram, W., Jitrapakdee, S., & Flegel, T. W. (2007). Journal of Virological Methods, 146, 125–128.CrossRefGoogle Scholar
  28. 28.
    Endo, S., Komori, T., Ricci, G., Sano, A., Yokoyama, K., Ohori, A., et al. (2004). FEMS Microbiology Letters, 234, 93–97.CrossRefGoogle Scholar
  29. 29.
    Tatibana, B. T., Sano, A., Uno, J., Kamei, K., Igarashi, T., Mikami, Y., et al. (2009). Journal of Clinical Laboratory Analysis, 23, 139–143.CrossRefGoogle Scholar
  30. 30.
    Kuboki, N., Inoue, N., Sakurai, T., Di Cello, F., Grab, D. J., Suzuki, H., et al. (2003). Journal of Clinical Microbiology, 41, 5517–5524.CrossRefGoogle Scholar
  31. 31.
    Thekisoe, O. M., Inoue, N., Kuboki, N., Tuntasuvan, D., Bunnoy, W., Borisutsuwan, S., et al. (2005). Veterinary Parasitology, 130, 327–330.CrossRefGoogle Scholar
  32. 32.
    Thekisoe, O. M., Kuboki, N., Nambota, A., Fujisaki, K., Sugimoto, C., Igarashi, I., et al. (2007). Acta Tropica, 102, 182–189.CrossRefGoogle Scholar
  33. 33.
    Han, E. T., Watanabe, R., Sattabongkot, J., Khuntirat, B., Sirichaisinthop, J., Iriko, H., et al. (2007). Journal of Clinical Microbiology, 45, 2521–2528.CrossRefGoogle Scholar
  34. 34.
    Alhassan, A., Thekisoe, O. M., Yokoyama, N., Inoue, N., Motloang, M. Y., Mbati, P. A., et al. (2007). Veterinary Parasitology, 143, 155–160.CrossRefGoogle Scholar
  35. 35.
    Ikadai, H., Tanaka, H., Shibahara, N., Matsuu, A., Uechi, M., Itoh, N., et al. (2004). Journal of Clinical Microbiology, 42, 2465–2469.CrossRefGoogle Scholar
  36. 36.
    Karanis, P., Thekisoe, O., Kiouptsi, K., Ongerth, J., Igarashi, I., & Inoue, N. (2007). Applied and Environmental Microbiology, 73, 5660–5662.CrossRefGoogle Scholar
  37. 37.
    El-Matbouli, M., & Soliman, H. (2005). Journal of Fish Diseases, 28, 549–557.CrossRefGoogle Scholar
  38. 38.
    Saito, R., Misawa, Y., Moriya, K., Koike, K., Ubukata, K., & Okamura, N. (2005). Journal of Medical Microbiology, 54, 1037–1041.CrossRefGoogle Scholar
  39. 39.
    Kawai, Y., Miyashita, N., Kishi, F., Tabuchi, M., Oda, K., Yamaguchi, T., et al. (2009). European Journal of Clinical Microbiology & Infectious Diseases, 28, 801–805.CrossRefGoogle Scholar
  40. 40.
    Fukuta, S., Mizukami, Y., Ishida, A., Ueda, J., Hasegawa, M., Hayashi, I., et al. (2004). European Food Research and Technology, 218, 496–500.CrossRefGoogle Scholar
  41. 41.
    Lee, D., La Mura, M., Allnutt, T. R., & Powell, W. (2009). BMC Biotechnology, 9, 7.CrossRefGoogle Scholar
  42. 42.
    Guan, X. Y., Guo, J. C., Shen, P., Yang, L. T., & Zhang, D. B. (2010). Food Analytical Methods. doi:10.1007/s12161-010-9132-x.Google Scholar
  43. 43.
    Hirayama, H., Kageyama, S., Moriyasu, S., Sawai, K., Onoe, S., Takahashi, Y., et al. (2004). Theriogenology, 62, 887–896.CrossRefGoogle Scholar
  44. 44.
    Hirayama, H., Kageyama, S., Takahashi, Y., Moriyasu, S., Sawai, K., Onoe, S., et al. (2006). Theriogenology, 66, 1249–1256.CrossRefGoogle Scholar
  45. 45.
    Horibe, D., Ochiai, T., Shimada, H., Tomonaga, T., Nomura, F., Gun, M., et al. (2006). International Journal of Cancer, 120, 1063–1069.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Shijun Fu
    • 1
    • 2
  • Guanggang Qu
    • 1
    • 3
  • Shijin Guo
    • 1
    • 2
  • Lin Ma
    • 2
  • Na Zhang
    • 3
  • Songlin Zhang
    • 1
  • Sanyang Gao
    • 1
    • 3
  • Zhiqiang Shen
    • 1
    • 2
    • 3
  1. 1.Key Laboratory of Preventive Veterinary Medicine and Animal BiotechnologyBinzhou Animal Husbandry and Veterinary Research AcademyBinzhouPeople’s Republic of China
  2. 2.Shandong Lvdu Ante Veterinary Drug Industry Co., LtdBinzhouPeople’s Republic of China
  3. 3.Shandong Lvdu Biological Technology Co., LtdBinzhouPeople’s Republic of China

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