Applied Biochemistry and Biotechnology

, Volume 163, Issue 5, pp 600–611 | Cite as

Rhamnolipid Production by Pseudomonas Aeruginosa GIM 32 Using Different Substrates Including Molasses Distillery Wastewater

  • An-hua Li
  • Mei-ying Xu
  • Wei Sun
  • Guo-ping SunEmail author


A rhamnolipid production strain newly isolated from oil-contaminated soil was identified as Pseudomonas aeruginosa GIM32 by its morphology and 16S rDNA sequence analysis. The effect of carbon source and carbon to nitrogen (C/N) ratio on rhamnolipids production was investigated. Palm oil was favorable as a carbon source for rhamnolipid production. The maximum biomass and rhamnolipid concentration were 8.24 g/L and 30.4 g/L, respectively, with an optimization medium containing 50 g/L palm oil and 5 g/L sodium nitrate. Molasses distillery wastewater as an unconventional substrate for rhamnolipid production was investigated. It was found that 2.6 g/L of rhamnolipids was produced; this amount was higher than that of past reports using wastewater as a substrate. In addition, 44% of the chemical oxygen demand of wastewater was removed at the same time under the optimization condition. Eleven kinds of different molecular weight rhamnolipid homologues were identified in the rhamnolipids obtained from molasses distillery wastewater by P. aeruginosa GIM32 by LC–MS analysis.


Rhamnolipids Pseudomonas aeruginosa Molasses distillery wastewater Biosurfactant 



This research is supported by Teamwork Project of the Natural Science Foundation of Guangdong Province (9351007002000001), Guangdong Provincial Programs for Promoting the Integration of Production, Teaching and Research (2009B090300300299), and Guangdong-Hongkong Technology Cooperation Funding (2008A030700003).


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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.South China Botanical GardenChinese Academy of SciencesGuangzhouChina
  2. 2.Guangdong Institute of MicrobiologyGuangdong Provincial Key Laboratory of Microbial Culture Collection and Application GuangzhouGuangzhouChina

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