Applied Biochemistry and Biotechnology

, Volume 162, Issue 3, pp 855–870

Genome-Wide Expression Changes in Saccharomyces cerevisiae in Response to High-LET Ionizing Radiation

  • Satomi Mizukami-Murata
  • Hitoshi Iwahashi
  • Shinzou Kimura
  • Kumie Nojima
  • Yoshinori Sakurai
  • Takeshi Saitou
  • Noriko Fujii
  • Yoshinori Murata
  • Shinzi Suga
  • Kazuhide Kitagawa
  • Kenichi Tanaka
  • Satoru Endo
  • Masaharu Hoshi
Article

DOI: 10.1007/s12010-009-8825-3

Cite this article as:
Mizukami-Murata, S., Iwahashi, H., Kimura, S. et al. Appl Biochem Biotechnol (2010) 162: 855. doi:10.1007/s12010-009-8825-3

Abstract

To understand the yeast response to high-linear energy transfer (LET) ionizing radiation (IR), we investigated global gene expression in yeast irradiated by three types of high-LET IR (fast neutrons, heavy ions, and thermal neutrons) and gamma rays using DNA microarray analysis. Stationary cells were irradiated by each IR and recultured in yeast–peptone–dextrose medium to allow repair for 40 min. RNA was then isolated from three independent samples of irradiated yeast. Genes involved in the Mec1p kinase pathway, which functions in DNA damage response, were induced by all forms of high-LET IR and by gamma rays. Some genes related to oxidative stress and the cell wall were induced by all forms of high-LET IRs. Gene expression patterns as a function of each type of high-LET IR were examined statistically by one-way analysis of variance. This analysis demonstrated the existence of irradiation-specific responses. For example, genes involved in ribosomal DNA synthesis were specifically induced by fast neutron irradiation, while the ubiquitin–proteasome system and heat shock response were specifically induced by thermal neutron irradiation. The study characterizes high-LET IR-induced gene expression and provides a molecular understanding of subsequent adaptation in yeast.

Keywords

High-LET IRs Fast neutron Heavy ion Thermal neutron Gene expression ANOVA analysis Mec1p kinase pathway Ubiquitin–proteasome system 

Copyright information

© Humana Press 2009

Authors and Affiliations

  • Satomi Mizukami-Murata
    • 1
  • Hitoshi Iwahashi
    • 1
  • Shinzou Kimura
    • 2
  • Kumie Nojima
    • 3
  • Yoshinori Sakurai
    • 4
  • Takeshi Saitou
    • 4
  • Noriko Fujii
    • 4
  • Yoshinori Murata
    • 5
  • Shinzi Suga
    • 6
  • Kazuhide Kitagawa
    • 6
  • Kenichi Tanaka
    • 6
  • Satoru Endo
    • 6
  • Masaharu Hoshi
    • 6
  1. 1.Human Stress Signal Research Center (HSS)National Institute of Advanced Industrial Science and Technology (AIST)IbarakiJapan
  2. 2.National Institute of Occupational Safety and HealthKanagawaJapan
  3. 3.National Institute of Radiological ScienceChibaJapan
  4. 4.Research Reactor InstituteKyoto UniversityOsakaJapan
  5. 5.Japan International Research Center for Agricultural SciencesIbarakiJapan
  6. 6.Hiroshima University Research Institute for Radiation Biology and MedicineHiroshimaJapan

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