Applied Biochemistry and Biotechnology

, Volume 162, Issue 2, pp 416–428 | Cite as

Microwave Exposure Affecting Reproductive System in Male Rats

  • Kavindra Kumar Kesari
  • Jitendra Behari


The object of present study is to investigate the effects of 50 GHz microwave frequency electromagnetic fields on reproductive system of male rats. Male rats of Wistar strain were used in the study. Animals 60 days old were divided into two groups—group I sham exposed and group II experimental (microwave exposed). During exposure, rats were confined in Plexiglas cages with drilled ventilation holes for 2 h a day for 45 days continuously at a specified specific absorption rate of 8.0 × 10−4 W/kg. After the last exposure, the rats were sacrificed immediately and sperms were collected. Antioxidant enzyme (superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase), histone kinase, apoptosis, and cell cycle were analyzed in sperm cells. Result shows a significant decrease in the level of sperm GPx and SOD activity (p ≤ 0.05), whereas catalase shows significant increase in exposed group of sperm samples as compared with control (p < 0.02). We observed a statistically significant decrease in mean activity of histone kinase as compared to the control (p < 0.016). The percentage of cells dividing in a spermatogenesis was estimated by analyzing DNA per cell by flow cytometry. The percentage of apoptosis in electromagnetic field exposed group shows increased ratio as compared to sham exposed (p < 0.004). There were no significant differences in the G0/G1 phase; however, a significant decrease (p < 0.026) in S phase was obtained. Results also indicate a decrease in percentage of G2/M transition phase of cell cycle in exposed group as compared to sham exposed (p < 0.019). We conclude that these radiations may have a significant effect on reproductive system of male rats, which may be an indication of male infertility.


Microwave radiation Histone kinase Cell cycle Flow cytometer Antioxidant 



Authors are thankful to the Council for Scientific and Industrial Research (CSIR) and Indian Council for Medical Research (ICMR), New Delhi, for the financial assistance.


  1. 1.
    Kesari, K. K., & Behari, J. (2009a). Fifty-gigahertz microwave exposure effect of radiations on rat brain. Applied Biochemistry and Biotechnology, 158(2), 126–139.CrossRefGoogle Scholar
  2. 2.
    Paulraj, R., & Behari, J. (2006). Single strand DNA breaks in rat brain cells exposed to microwave radiation. Mutation Research, 596, 76–80. doi: 10.1016/j.mrfmmm.2005.12.006.Google Scholar
  3. 3.
    Paulraj, R., & Behari, J. (2004). Radiofrequency radiation effect on protein kinase C activity in rats brain. Mutation Research, 585, 127–131.Google Scholar
  4. 4.
    Deepinder, F., Kartikeya Makkar, K., & Agarwal, A. (2007). Cell phones and male infertility: dissecting the relationship. Reproductive Biomedicine Online, 15(3), 266–270.Google Scholar
  5. 5.
    Fejes, I., Zavaczki, Z., Szollosi, J., Koloszar, S., Daru, J., Kovacs, L., et al. (2005). Is there a relationship between cell phone use and semen quality? Archives of Andrology, 51, 385–393.CrossRefGoogle Scholar
  6. 6.
    Agarwal, A., Deepinder, F., Sharma, R. K., Ranga, G., & Li, J. (2008). Effect of cell phone usage on semen analysis in men attending infertility clinic: an observational study. Fertility and Sterility, 89(1), 124–128.CrossRefGoogle Scholar
  7. 7.
    Davoudi, M., Brossner, C., & Kuber, W. (2002). The influence of electromagnetic waves on sperm motility. Journal für Urologie und Urogynäkologie, 19, 18–32.Google Scholar
  8. 8.
    Erogul, O., Oztas, E., Yildirim, I., Kir, T., Aydur, E., Komesli, G., et al. (2006). Effects of electromagnetic radiation from a cellular phone on human sperm motility: an in vitro study. Archives of Medical Research, 37, 840–843.CrossRefGoogle Scholar
  9. 9.
    Dasdag, S., Ketani, M. A., Akdag, Z., et al. (1999). Whole-body microwave exposure emitted by cellular phones and testicular function of rats. Urology Research, 27, 219–223.CrossRefGoogle Scholar
  10. 10.
    Dasdag, S., Akdag, M. Z., Aksen, F., et al. (2003). Whole body exposure of rats to microwaves emitted from a cell phone does not affect the testes. Bioelectromagnetics, 24, 182–188.CrossRefGoogle Scholar
  11. 11.
    Behari, J., & Kesari, K. K. (2006). Effects of microwave radiations on reproductive system of male rats. Embryo Talk, 1, 81–85.Google Scholar
  12. 12.
    Sikka, S. C. (1996). Oxidative stress and role of antioxidants in normal and abnormal sperm function. Frontiers in Bioscience, 1, e78–e86.Google Scholar
  13. 13.
    Miesel, R., Jedrzejez, K., Sanocka, D., & kKurpisz, M. K. (1997). Severe antioxidase deficiency in human semen samples with pathological spermiogram parameters. Andrologia, 29, 77–83.CrossRefGoogle Scholar
  14. 14.
    Murphy, J. C., Kaden, D. A., Warren, J., & Sivak, A. (1993). International Commission for Protection against Environmental Mutagens and Carcinogens. Power frequency electric and magnetic fields: a review of genetic toxicology. Mutation Research, 296, 221–240.Google Scholar
  15. 15.
    Halliwell, B., & Gutteridge, J. M. C. (1999). Free radicals, other reactive species and disease. In B. Halliwell & J. M. C. Gutteridge (Eds.), Free radicals in Biology and Medicine (3rd ed., pp. 639–645). New York: Oxford University Press.Google Scholar
  16. 16.
    Nazıroglu, M., Karaoglu, A., & Aksoy, A. O. (2004). Selenium and high dose vitamin E administration protects cisplatin-induced oxidative damage to renal, liver and lens tissues in rats. Toxicology, 195, 221–230.CrossRefGoogle Scholar
  17. 17.
    Moustafa, Y. M., Moustafa, R. M., Beacy, A., Abou-El-Ela, S. H., & Ali, F. M. (2001). Effects of acute exposure to the radiofrequency fields of cellular phones on plasma lipid peroxide and antioxidase activities in human erythrocytes. Journal of Pharmaceutical and Biomedical Analysis, 26, 605–608.CrossRefGoogle Scholar
  18. 18.
    Rush, G. F., Gorski, J. R., Ripple, M. G., Sowinski, J., Bugelski, P., & Hewitt, W. R. (1985). Organic hydroperoxide-induced lipid peroxidation and cell death in isolated hepatocytes. Toxicology and Applied Pharmacology, 78, 473–483.CrossRefGoogle Scholar
  19. 19.
    Spano, M., & Evenson, D. P. (1993). Flow cytometric analysis for reproductive biology. Biology of the Cell, 78, 53–62.CrossRefGoogle Scholar
  20. 20.
    Nunez, R. (2001). DNA measurement and cell cycle analysis by flow cytometry. Current Issues in Molecular Biology, 3(3), 67–70.Google Scholar
  21. 21.
    Durney, C. H., Iskander, M. F., Massoudi, H., Johnson, C. C. (1984). An empirical formula for broad band SAR calculations of prolate spheroidal models of humans and animal. In: J. M. Osepchuk (Ed.), Biological effects of electromagnetic radiation (pp. 85–90). New York: IEEE Press.Google Scholar
  22. 22.
    Lowry, O. H., Resebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with folinphenol reagent. Journal of Biological Chemistry, 193, 265–275.Google Scholar
  23. 23.
    Chen, G., Upham, B. L., Sun, W., et al. (2000). Effect of electromagnetic field exposure on chemically induced differentiation of friend erythroleukemia cells. Environmental Health Perspectives, 108, 967–972.CrossRefGoogle Scholar
  24. 24.
    Brydon, L., Petit, L., Delagrange, P., Strosberg, A. D., & Jockers, R. (2000). Functional expression of MT2 melatonin receptors in human PAZ6 adipocutes. Endocrinology, 142, 4264–4271.CrossRefGoogle Scholar
  25. 25.
    Plante, M., de Lamirande, E., & Gagnon, C. (1994). Reactive oxygen species released by activated neutrophils, but not by deficient spermatozoa, are sufficient to affect normal sperm motility. Fertility and Sterility, 62(2), 387–393.Google Scholar
  26. 26.
    Alvarez, J. G., Touchstone, J. C., Blasco, L., & Storey, B. T. (1987). Spontaneous lipid peroxidation and production of hydrogen peroxide and superoxide in human spermatozoa. Superoxide dismutase as major enzyme protectant against oxygen toxicity. Journal of Andrology, 8(5), 338–348.Google Scholar
  27. 27.
    Yen, G. C., Yeh, C. T., & Chen, Y. J. (2004). Protective effect of Mesona procumbens against tertbutyl hydroperoxide-induced acute hepatic damage in rats. Journal of Agricultural and Food Chemistry, 52, 4121–4127.CrossRefGoogle Scholar
  28. 28.
    Hunter, T., & Plowman, G. D. (1997). The protein kinases of budding yeast: six score and more. Trends in Biochemical Sciences, 22, 18–22.CrossRefGoogle Scholar
  29. 29.
    Manning, G., Whyte, D. B., Martinez, R., Hunter, T., & Sundersanam, S. (2002). The protein kinase complement of the human genome. Science, 298, 1912–1934.CrossRefGoogle Scholar
  30. 30.
    Hanks, S. K., Quinn, A. M., & Hunter, T. (1988). The protein kinase family: conserved features and deduced phylogeny of the catalytic domains. Science, 241, 42–52.CrossRefGoogle Scholar
  31. 31.
    Hanks, S. K., & Hunter, T. (1995). The eukaryotic protein kinase superfamily: kinase (catalytic) domain structure and classification. FASEB Journal, 9, 576–596.Google Scholar
  32. 32.
    Doree, M. (1990). Control of M-phase by maturation-promoting factor. Current Opinion in Cell Biology, 2, 269–273.CrossRefGoogle Scholar
  33. 33.
    Maller, J. L. (1990). Xenopus oocytes and the biochemistry of cell division. Biochemistry, 29, 3157–3166.CrossRefGoogle Scholar
  34. 34.
    Nurse, P. (1990). Universal control mechanism regulating onset of M-phase. Nature, 344, 503–508.CrossRefGoogle Scholar
  35. 35.
    Meikrantz, W., & Schlegel, R. A. (1992). M-phase-promoting factor activation. Journal of Cell Science, 101, 475–481.Google Scholar
  36. 36.
    Jung, T., Moor, R. M., & Fulka, J. (1993). Kinetics of MPF and histone H1 kinase activity differ during the G2- to M-phase transition in mouse oocytes. International Journal of Developmental Biology, 37, 595–600.Google Scholar
  37. 37.
    Dunphy, W. G., Brizuela, L., Beach, D., & Newport, J. (1988). The Xenopus cdc2 protein is a component of MPF, a cytoplasmic regulator of mitosis. Cell, 54, 423–431.CrossRefGoogle Scholar
  38. 38.
    Gautier, J., Norbury, C., Lohka, M., Nuese, P., & Mailer, J. (1988). Purified maturation-promoting factor contains the product of a Xenopus homolog of the fission yeast cell cycle control gene cdc2. Cell, 54, 433–439.CrossRefGoogle Scholar
  39. 39.
    Labbe, J. C., Capony, J. P., Caput, D., Cavadore, J. C., Derancourt, J., Kaghad, M., et al. (1989). MPF from starfish oocytes at first meiotic metaphase is a heterodimer containing one molecule of cdc2 and one molecule of cyclin B. Embo Journal, 8, 3053–3058.Google Scholar
  40. 40.
    Pawse, A. R., Margery, G. O., & Stocken, L. A. (1971). Histone kinase and cell division. Biochemical Journal, 122, 713–719.Google Scholar
  41. 41.
    Ozturk, M. A., Karcaaltincaba, M., & Criss, W. E. (1993). Cell cycle control part I cdc related kinases. Journal of Islamic Academy of Sciences, 6(4), 311–318.Google Scholar
  42. 42.
    Kesari, K. K., & Behari, J. (2009b). Effect of microwave at 2.45 GHz radiations on reproductive system of male rats. Toxicological and Environmental Chemistry. (in press).Google Scholar

Copyright information

© Humana Press 2009

Authors and Affiliations

  1. 1.Bioelectromagnetic Laboratory, School of Environmental SciencesJawaharlal Nehru UniversityNew DelhiIndia

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