Bleach Enhancement of Mixed Wood Pulp by Xylanase–Laccase Concoction Derived Through Co-culture Strategy

  • Pallavi Dwivedi
  • V. Vivekanand
  • Nidhi Pareek
  • Amit Sharma
  • Rajesh P. Singh


Mixed enzyme preparation having both xylanase and laccase activity was evaluated for its bleach enhancing ability of mixed wood pulp. The enzyme was produced through co-cultivation of mutant Penicillium oxalicum SAUE-3.510 and Pleurotus ostreatus MTCC 1804 under solid-state fermentation. Bleaching of pulp with mixed enzyme had resulted into a notable decrease in kappa number and increased brightness as compared to xylanase alone. Analysis of bleaching conditions had denoted that 8 IU g−1 of mixed enzyme preparation (xylanase/laccase, 22:1) had led into maximal removal of lignin from pulp when bleaching was performed at 10% pulp consistency (55 °C, pH 9.0) for 3 h. An overall improvement of 21%, 8%, 3%, and 5% respectively in kappa number, brightness, yellowness, and viscosity of pulp was achieved under derived bleaching conditions. Process of enzymatic bleaching was further ascertained by analyzing the changes occurring in polysaccharide and lignin by HPLC and FTIR. The UV absorption spectrum of the compounds released during enzymatic treatment had denoted a characteristic peak at 280 nm, indicating the presence of lignin in released coloring matter. The changes in fiber morphology following enzymatic delignification were studied by scanning electron microscopy.


Biobleaching Penicillium oxalicum Co-cultivation Xylanase Laccase Pleurotus ostreatus 



Senior research fellowship awarded by Council of Scientific and Industrial Research, New Delhi, India to first author and national doctoral fellowship to second author by All India Council of Technical Education, New Delhi, India are gratefully acknowledged.


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Copyright information

© Humana Press 2009

Authors and Affiliations

  • Pallavi Dwivedi
    • 1
    • 2
  • V. Vivekanand
    • 1
    • 3
  • Nidhi Pareek
    • 1
  • Amit Sharma
    • 1
    • 4
  • Rajesh P. Singh
    • 1
  1. 1.Department of BiotechnologyIndian Institute of Technology RoorkeeRoorkeeIndia
  2. 2.Department of BiotechnologySir Padampat Singhania UniversityUdaipurIndia
  3. 3.Lundberg Laboratory, CMB-Molecular BiologyGothenburg UniversityGothenburgSweden
  4. 4.Division of GeneticsIndian Agricultural Research InstituteNew DelhiIndia

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