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Applied Biochemistry and Biotechnology

, Volume 160, Issue 1, pp 244–254 | Cite as

Succinic Acid Production by Actinobacillus succinogenes Using Spent Brewer's Yeast Hydrolysate as a Nitrogen Source

  • Min JiangEmail author
  • Kequan Chen
  • Zhongmin Liu
  • Ping Wei
  • Hanjie Ying
  • Honam Chang
Article

Abstract

To develop a cost-effective fermentation medium, spent brewer's yeast hydrolysate was evaluated as a nitrogen source for succinic acid production by Actinobacillus succinogenes NJ113 in glucose-containing media. Autolysis and enzymatic hydrolysis were used to hydrolyze the spent brewer's yeast cells to release the nutrients. The results showed that enzymatic hydrolysis was a more effective method due to the higher succinic acid yield and cell growth. However, the incomplete glucose consumption indicated existence of nutrient limitation. Vitamins were subsequently identified as the main limiting factors for succinic acid production using enzymatically hydrolyzed spent brewer's yeast as a nitrogen source. After the addition of vitamins, cell growth and succinic acid concentration both improved. As a result, 15 g/L yeast extract could be successfully replaced with the enzymatic hydrolysate of spent brewer's yeast with vitamins supplementation, resulting in a production of 46.8 g/L succinic acid from 68 g/L glucose.

Keywords

Succinic acid Actinobacillus succinogenes NJ113 Brewer's yeast hydrolysate Nitrogen source 

Notes

Acknowledgements

This work was supported by grant no. 2006AA02Z235 from “863” Program of China, grant no. 20606017 from National Natural Science Foundation of China, grant no. 2009CB724701 from “973” Program of China.

References

  1. 1.
    Bechthold, I., Bretz, K., Kabasci, S., Kopitzky, R., & Springer, A. (2008). Succinic acid: a new platform chemical for biobased polymers from renewable resources. Chemical Engineering & Technology, 31, 647–654. doi: 10.1002/ceat.200800063.CrossRefGoogle Scholar
  2. 2.
    McKinlay, J., Vieille, C., & Zeikus, J. G. (2007). Prospects for a bio-based succinate industry. Applied Microbiology and Biotechnology, 76, 727–740. doi: 10.1007/s00253-007-1057-y.CrossRefGoogle Scholar
  3. 3.
    Song, H., & Lee, S. Y. (2006). Production of succinic acid by bacterial fermentation. Enzyme and Microbial Technology, 39, 352–361. doi: 10.1016/j.enzmictec.2005.11.043.CrossRefGoogle Scholar
  4. 4.
    Willke, T., & Vorlop, K.-D. (2004). Industrial bioconversion of renewable resources as an alternative to conventional chemistry. Applied Microbiology and Biotechnology, 66, 131–142. doi: 10.1007/s00253-004-1733-0.CrossRefGoogle Scholar
  5. 5.
    Lee, P. C., Lee, S. Y., Hong, S. H., & Chang, H. N. (2002). Isolation and characterization of a new succinic acid-producing bacterium, Mannheimia succiniciproducens MBEL55E from bovine rumen. Applied Microbiology and Biotechnology, 58, 663–668. doi: 10.1007/s00253-002-0935-6.CrossRefGoogle Scholar
  6. 6.
    Kim, D. Y., Yim, S. C., Lee, P. C., & Lee, W. G. (2004). Batch and continuous fermentation of succinic acid from wood hydrolysate by Mannheimia succiniciproducens MBEL55E. Enzyme and Microbial Technology, 35, 648–653. doi: 10.1016/j.enzmictec.2004.08.018.CrossRefGoogle Scholar
  7. 7.
    Guettler, M. V., Rumler, D., & Jain, M. K. (1999). Actinobacillus succinogenes sp. nov., a novel succinic-acid-producing strain from the bovine rumen. International Journal of Systematic Bacteriology, 49, 207–216.CrossRefGoogle Scholar
  8. 8.
    Guettler, M.V., Jain, M.K., & Rumler, D. (1996). Method for making succinic acid, bacterial variants for use in the process, and methods for obtaining variants. US patent 5,504,004.Google Scholar
  9. 9.
    Van der Werf, M. J., Guettler, M. V., Jain, M. K., & Zeikus, J. G. (1997). Environmental and physiological factors affecting the succinate product ratio during carbohydrate fermentation by Actinobacillus sp. 130Z. Archives of Microbiology, 167, 332–342. doi: 10.1007/s002030050452.CrossRefGoogle Scholar
  10. 10.
    Urbance, S. E., Pometto, A. L., III, Dispirito, A. A., & Denli, Y. (2004). Evaluation of succinic acid continuous and repeat-batch biofilm fermentation by Actinobacillus succinogenes using plastic composite support bioreactors. Applied Microbiology and Biotechnology, 65, 664–670. doi: 10.1007/s00253-004-1634-2.CrossRefGoogle Scholar
  11. 11.
    Datta, R. (1992). Process for the production of succinic acid by anaerobic fermentation. US patent 5,143,833.Google Scholar
  12. 12.
    Lee, P. C., Lee, W. G., Lee, S. Y., & Chang, H. N. (1999). Effects of medium components on the growth of Anaerobiospirillum succiniciproducens and succinic acid production. Process Biochemistry, 35, 49–55. doi: 10.1016/S0032-9592(99)00031-X.CrossRefGoogle Scholar
  13. 13.
    Lee, P. C., Lee, W. G., Kwon, S., Lee, S. Y., & Chang, H. N. (1999). Succinic acid production by Anaerobiospirillum succiniciproducens: effects of the H2/CO2 supply and glucose concentration. Enzyme and Microbial Technology, 24, 549–554. doi: 10.1016/S0141-0229(98)00156-2.CrossRefGoogle Scholar
  14. 14.
    Vemuri, G. N., Eiteman, M. A., & Altman, E. (2002). Succinate production in dual-phase Escherichia coli fermentations depends on the time of transition from aerobic to anaerobic conditions. Journal of Industrial Microbiology & Biotechnology, 28, 325–332. doi: 10.1038/sj.jim.7000250.CrossRefGoogle Scholar
  15. 15.
    Lin, H., Bennett, G. N., & San, K. Y. (2005). Metabolic engineering of aerobic succinate production systems in Escherichia coli to improve process productivity and achieve the maximum theoretical succinate yield. Metabolic Engineering, 7, 116–127. doi: 10.1016/j.ymben.2004.10.003.CrossRefGoogle Scholar
  16. 16.
    Lee, P. C., Lee, S. Y., Hong, S. H., Chang, H. N., & Park, S. C. (2003). Biological conversion of wood hydrolysate to succinic acid by Anaerobiospirillum succiniciproducens. Biotechnology Letters, 25, 111–114. doi: 10.1023/A:1021907116361.CrossRefGoogle Scholar
  17. 17.
    Urbance, S. E., Pometto, A. L., III, DiSpirito, A. A., & Demirci, A. (2003). Medium evaluation and plastic composite support ingredient selection for biofilm formation and succinic acid production by Actinobacillus succinogenes. Food Biotechnology, 17, 53–65. doi: 10.1081/FBT-120019984.CrossRefGoogle Scholar
  18. 18.
    Xu, H., Liu, J. J., & Zhao, X. Y. (2008). Comprehensive utilization of brewer's yeast. Liquor Making, 35, 47–49. in Chinese.Google Scholar
  19. 19.
    York, S. W., & Ingram, L. O. (1996). Ethanol production by recombinant Escherichia coli KO11 using crude yeast autolysate as a nutrient supplement. Biotechnology Letters, 18, 683–688. doi: 10.1007/BF00130766.CrossRefGoogle Scholar
  20. 20.
    Rivas, B., Moldes, A. B., Dominguez, J. M., & Parajo, J. C. (2004). Development of culture media containing spent yeast cells of Debaryomyces hansenii and corn steep liquor for lactic acid production with Lactobacillus rhamnosus. International Journal of Food Microbiology, 97, 93–98. doi: 10.1016/j.ijfoodmicro.2004.05.006.CrossRefGoogle Scholar
  21. 21.
    Marica, R., Maja, V., Slavica, S. M., & Milan, M. (2007). Contribution of lactic acid fermentation to improved nutritive quality vegetable juiced enriched with brewer's yeast autolysate. Food Chemistry, 100, 599–602. doi: 10.1016/j.foodchem.2005.09.077.CrossRefGoogle Scholar
  22. 22.
    McKinlay, J. B., Zeikus, J. G., & Vieille, C. (2005). Insights into Actinobacillus succinogenes fermentative metabolism in a chemically defined growth medium. Applied and Environmental Microbiology, 71, 6651–6656. doi: 10.1128/AEM.71.11.6651-6656.2005.CrossRefGoogle Scholar
  23. 23.
    Press editors. (2003). National standard assembly of China. Beijing: Standard Press of China.Google Scholar
  24. 24.
    Lee, P. C., Lee, S. Y., Hong, S. H., & Chang, H. N. (2003). Batch and continuous cultures of Mannheimia succiniciproducens MBEL55E for the production of succinic acid from whey and corn steep liquor. Bioprocess and Biosystems Engineering, 26, 63–67. doi: 10.1007/s00449-003-0341-1.CrossRefGoogle Scholar
  25. 25.
    Liu, Y. P., Zheng, P., Sun, Z. H., Ni, Y., Dong, J. J., & Zhu, L. L. (2007). Economical succinic acid production from cane molasses by Actinobacillus succinogenes. Bioresource Technology, 99, 1736–1742. doi: 10.1016/j.biortech.2007.03.044.CrossRefGoogle Scholar
  26. 26.
    Agarwal, L., Isar, J., Meghwanshi, G. K., & Saxena, R. K. (2006). A cost effective fermentative production of succinic acid from cane molasses and corn steep liquor by Escherichia coli. Journal of Applied Microbiology, 100, 1348–1354. doi: 10.1111/j.1365-2672.2006.02894.x.CrossRefGoogle Scholar
  27. 27.
    Champagne, C. P., Barrette, J., & Goulet, J. (1999). Interaction between pH, autolysis promoters and bacterial contamination on the production of yeast extracts. Food Research International, 32, 575–583. doi: 10.1016/S0963-9969(99)00133-7.CrossRefGoogle Scholar
  28. 28.
    Tatjana, V. M., Marica, R., & Slavica, I. M. (2007). Utilization of baker's yeast (Saccharomyces cerevisiae) for the production of yeast extract: effects of different enzymatic treatments on solid, protein and carbohydrate recovery. Journal of the Serbian Chemical Society, 72, 451–457. doi: 10.2298/JSC0705451V.CrossRefGoogle Scholar
  29. 29.
    John, R. P., Nampoothiri, K. M., & Pandey, A. (2007). Fermentative production of lactic acid from biomass: an overview on process developments and future perspectives. Applied Microbiology and Biotechnology, 74, 524–534. doi: 10.1007/s00253-006-0779-6.CrossRefGoogle Scholar
  30. 30.
    Kwon, S., Lee, P. C., Lee, E. G., Chang, Y. K., & Chang, N. (2000). Production of lactic acid by Lactobacillus rhamnosus with vitamin-supplemented soybean hydrolysate. Enzyme and Microbial Technology, 26, 209–215. doi: 10.1016/S0141-0229(99)00134-9.CrossRefGoogle Scholar
  31. 31.
    Li, Z., Ding, S. F., Li, Z. P., & Tan, T. W. (2006). l-Lactic acid production by lactobacillus casei fermentation with corn steep liquor-supplemented acid-hydrolysate of soybean meal. Biotechnology Journal, 1, 1453–1458. doi: 10.1002/biot.200600099.CrossRefGoogle Scholar
  32. 32.
    Gao, M. T., Hirata, M., Toorisaka, E., & Hano, T. (2007). Lactic acid production with the supplementation of spent cells and fish wastes for the purpose of reducing impurities in fermentation broth. Biochemical Engineering Journal, 36, 276–280. doi: 10.1016/j.bej.2007.02.030.CrossRefGoogle Scholar
  33. 33.
    Gao, M. T., Hirata, M., Toorisaka, E., & Hano, T. (2006). Study on acid-hydrolysis of spent cells for lactic acid fermentation. Biochemical Engineering Journal, 28, 87–91. doi: 10.1016/j.bej.2005.09.006.CrossRefGoogle Scholar
  34. 34.
    Du, C., Carol Lin, K. C., Koutinas, A., Wang, R., & Webb, C. (2007). Succinic acid production from wheat using a biorefining strategy. Applied Microbiology and Biotechnology, 76, 1263–1270. doi: 10.1007/s00253-007-1113-7.CrossRefGoogle Scholar

Copyright information

© Humana Press 2009

Authors and Affiliations

  • Min Jiang
    • 1
    Email author
  • Kequan Chen
    • 1
  • Zhongmin Liu
    • 1
  • Ping Wei
    • 1
  • Hanjie Ying
    • 1
  • Honam Chang
    • 2
  1. 1.State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and PharmacyNanjing University of TechnologyNanjingPeople’s Republic of China
  2. 2.Department of Chemical and Biomolecular EngineeringKorea Advanced Institute of Science and TechnologyDaejeonRepublic of Korea

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