Applied Biochemistry and Biotechnology

, Volume 160, Issue 5, pp 1341–1355

Alpha-Glucosidase Folding During Urea Denaturation: Enzyme Kinetics and Computational Prediction

  • Xue-Qiang Wu
  • Jun Wang
  • Zhi-Rong Lü
  • Hong-Min Tang
  • Daeui Park
  • Sang-Ho Oh
  • Jong Bhak
  • Long Shi
  • Yong-Doo Park
  • Fei Zou
Article

DOI: 10.1007/s12010-009-8636-6

Cite this article as:
Wu, XQ., Wang, J., Lü, ZR. et al. Appl Biochem Biotechnol (2010) 160: 1341. doi:10.1007/s12010-009-8636-6

Abstract

In this study, we investigated structural changes in alpha-glucosidase during urea denaturation. Alpha-glucosidase was inactivated by urea in a dose-dependent manner. The inactivation was a first-order reaction with a monophase process. Urea inhibited alpha-glucosidase in a mixed-type reaction. We found that an increase in the hydrophobic surface of this enzyme induced by urea resulted in aggregation caused by unstable folding intermediates. We also simulated the docking between alpha-glucosidase and urea. The docking simulation suggested that several residues, namely THR9, TRP14, LYS15, THR287, ALA289, ASP338, SER339, and TRP340, interact with urea. Our study provides insights into the alpha-glucosidase unfolding pathway and 3D structure of alpha-glucosidase.

Keywords

Alpha-glucosidase Urea unfolding Docking simulation 

Abbreviations

pNPG

p-Nitrophenyl α-d-glucopyranoside

pNP

4-Nitrophenol

SDS

Sodium dodecyl sulfate

ANS

1-Anilino-8-naphthalenesulfonate

Copyright information

© Humana Press 2009

Authors and Affiliations

  • Xue-Qiang Wu
    • 1
  • Jun Wang
    • 2
  • Zhi-Rong Lü
    • 1
  • Hong-Min Tang
    • 3
  • Daeui Park
    • 4
  • Sang-Ho Oh
    • 4
  • Jong Bhak
    • 4
  • Long Shi
    • 1
  • Yong-Doo Park
    • 1
    • 3
  • Fei Zou
    • 1
  1. 1.Department of Environmental Health, School of Public Health and Tropical MedicineSouthern Medical UniversityGuangzhouPeople’s Republic of China
  2. 2.School of MedicineShenzhen UniversityShenzhenPeople’s Republic of China
  3. 3.Yangtze Delta Region Institute of Tsinghua UniversityJiaxingPeople’s Republic of China
  4. 4.Korean BioInformation Center (KOBIC)KRIBBDaejeonSouth Korea

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