Improved Production of Ethanol by Novel Genome Shuffling in Saccharomyces cerevisiae
- 936 Downloads
Fermentation properties under the control of multiple genes of industrial Saccharomyces cerevisiae strain are difficult to alter with traditional methods. Here, we describe efficient and reliable genome shuffling to increase ethanol production through the rapid improvement of stress resistance. The strategy is carried out using yeast sexual and asexual reproduction by itself instead of polyethylene glycol-mediated protoplast fusion. After three rounds of genome shuffling, the best performing strain S3-10 was obtained on the special plate containing a high ethanol concentration. It exhibits substantial improvement in multiple stress tolerance to ethanol, glucose, and heat. The cycle of fermentation of S3-10 was not only shortened, but also, ethanol yield was increased by up to 10.96% compared with the control in very-high-gravity (VHG) fermentations. In total, S3-10 possesses optimized fermentation characteristics, which will be propitious to the development of bioethanol fermentation industry.
KeywordsEthanol Genome shuffling Industrial strain Saccharomyces cerevisiae
ethyl methane sulfonate
high-performance liquid chromatography
very high gravity
The author particularly thanks Prof. P. Ma for constructive advice on this work. The research was supported by the National Natural Science Foundation of China (no. 30470849).
- 2.Boulton, B., Singleton, V. L., Bisson, L. F., & Kunkee, R. E. (1996). Yeast and biochemistry of ethanol fermentation. In B. Boulton, V. L. Singleton, L. F. Bisson, & R. E. Kunkee (Eds.), Principles and practices of winemaking (pp. 139–172). New York: Chapman and Hall.Google Scholar
- 14.Lawrence, C. W. (2004). Guide to yeast genetics and molecular and cell biology. Methods in enzymology Part A. Elesevier academic press NK, 194.Google Scholar
- 15.Houston, P., Simon, P. J., & Broach, J. R. (2004). The saccharomyces cerevisiae recombination enhancer biases recombination during interchromosomal mating-type switching but not in interchromosomal homologous recombination. Genetics, 166, 1187–1197. doi: 10.1534/genetics.166.3.1187.CrossRefGoogle Scholar
- 16.Spencer, J. F. T., & Spencer, D. M. (1996). Yeast Protocols: Methods in Cell and Molecular Biology, Mutagenesis in yeast. 17–18.Google Scholar
- 17.Carlson, C. R., Grallert, B., Bernander, R., Stokke, T., & Boye, E. (1997). Measurement of nuclear DNA content in fission yeast by flow cytometry. Yeast (Chichester, England), 13, 1329–1335. doi: 10.1002/(SICI)1097-0061(199711)13:14<1329::AID-YEA185>3.0.CO;2-M.CrossRefGoogle Scholar