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Applied Biochemistry and Biotechnology

, Volume 160, Issue 4, pp 1084–1093 | Cite as

Improved Production of Ethanol by Novel Genome Shuffling in Saccharomyces cerevisiae

  • Lihua HouEmail author
Article

Abstract

Fermentation properties under the control of multiple genes of industrial Saccharomyces cerevisiae strain are difficult to alter with traditional methods. Here, we describe efficient and reliable genome shuffling to increase ethanol production through the rapid improvement of stress resistance. The strategy is carried out using yeast sexual and asexual reproduction by itself instead of polyethylene glycol-mediated protoplast fusion. After three rounds of genome shuffling, the best performing strain S3-10 was obtained on the special plate containing a high ethanol concentration. It exhibits substantial improvement in multiple stress tolerance to ethanol, glucose, and heat. The cycle of fermentation of S3-10 was not only shortened, but also, ethanol yield was increased by up to 10.96% compared with the control in very-high-gravity (VHG) fermentations. In total, S3-10 possesses optimized fermentation characteristics, which will be propitious to the development of bioethanol fermentation industry.

Keywords

Ethanol Genome shuffling Industrial strain Saccharomyces cerevisiae 

Abbreviations

PEG

polyethylene glycol

EMS

ethyl methane sulfonate

CFU

colony-forming units

HPLC

high-performance liquid chromatography

FCAS

flow-cytometry analysis

VHG

very high gravity

Notes

Acknowledgments

The author particularly thanks Prof. P. Ma for constructive advice on this work. The research was supported by the National Natural Science Foundation of China (no. 30470849).

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Copyright information

© Humana Press 2009

Authors and Affiliations

  1. 1.Department of Biochemical Engineering, School of Chemical Engineering and TechnologyTianjin UniversityTianjinPeople’s Republic of China

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