Aspergillus fumigatus Thermophilic and Acidophilic Endoglucanases
- 340 Downloads
This study evaluated the production of cellulolytic enzymes by an Aspergillus fumigatus strain, isolated from sugar cane bagasse, according to its ability to grow on microcrystalline cellulose as the sole carbon source. The effect of the carbon source (brewer’s spent grain, sugarcane bagasse, and wheat bran) and of the nitrogen source (corn steep liquor and sodium nitrate) on cellulase production was studied using submerged and solid state cultivations at 30 °C. The highest levels of endoglucanase (CMCase) corresponded to 365 U L-1 and was obtained using sugarcane bagasse (1%) and corn steep liquor (1.2%) in submerged fermentation within 6 days of cultivation. This supernatant was used to run a sodium dodecyl sulfate polyacrylamide gel electrophoresis that showed six bands with endoglucanase activity. CMCase activity was higher at 65 °C and pH 2.0, indicating that this microorganism produces a thermophilic and acid endoglucanase. Solid state cultivation favored FPase production, that reached 47 U g-1 of dry substrate (wheat bran and sugarcane bagasse) within 3 days.
KeywordsAspergillus fumigatus Thermophilic endoglucanase Acidophilic endoglucanase CMCase Agro-industrial by-products
Authors are indebted to Marta de Sousa Ferreira for technical support and Fundação Oswaldo Cruz for identification of the fungal strain. This work was financially supported by CNPq and FINEP (project no 0106004700).
- 1.Berlin, A., Gilkes, N., Kilburn, D., Bura, R., Markov, A., Skomarovsky, A., et al. (2005). Evaluation of novel fungal cellulase preparations for ability to hydrolyze softwood substrates—evidence for the role of accessory enzymes. Enzyme and Microbial Technology, 37, 175–184. doi: 10.1016/j.enzmictec.2005.01.039.CrossRefGoogle Scholar
- 4.Novozymes, NREL make further biomass-to-ethanol progress. In: Ethanol producer magazine. USA: BBI International; May 14, 2004.Google Scholar
- 5.Pothiraj, C., Balaji, P., & Eyini, M. (2006). Enhanced production of cellulases by various fungal cultures in solid state fermentation of cassava waste. African Journal of Biotechnology, 20, 1882–1885.Google Scholar
- 6.Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., et al. (1985). Genetic manipulation of Streptomyces. A laboratory manual. Norwich, UK: The John Innes Institute.Google Scholar
- 9.Nascimento, R. P., Coelho, R. R. R., Marques, S., Alves, L., Gírio, F. M., Bon, E. P. S., et al. (2002). Production and partial characterisation of xylanase from Streptomyces sp. strain AMT-3 isolated from Brazilian cerrado soil. Enzyme and Microbial Technology, 31, 549–555. doi: 10.1016/S0141-0229(02)00150-3.CrossRefGoogle Scholar
- 11.Dahot, M. U., & Noomrio, M. H. (1996). Microbial production of cellulases by Aspergillus fumigatus using wheat straw as a carbon source. Journal of Islamic Academy of Sciences, 9, 119–124.Google Scholar
- 13.Gao, J., Weng, H., Zhu, D., Yuan, M., Guan, F., & Xi, Y. (2008). Production and characterization of cellulolytic enzymes from the thermoacidophilic fungal Aspergillus terreus M11 under solid-state cultivation of corn stover. Bioresource Technology, 99, 7623–7629.Google Scholar
- 14.Grigorevski-Lima, A. L., Nascimento, R. P., Bon, E. P. S., & Coelho, R. R. R. (2005). Streptomyces drozdowiczii cellulase production using agro-industrial by-products and its potential use in the detergent and textile industries. Enzyme and Microbial Technology, 37, 272–277. doi: 10.1016/j.enzmictec.2005.03.016.CrossRefGoogle Scholar
- 18.Aguiar, C. L. (2001). Biodegradation of the cellulose from sugar cane bagasse by fungal cellulase. Ciência e Tecnologia de Alimentos, 3, 117–121.Google Scholar