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Aspergillus fumigatus Thermophilic and Acidophilic Endoglucanases

  • A. L. Grigorevski-Lima
  • F. N. M. Da Vinha
  • D. T. Souza
  • A. S. R. Bispo
  • E. P. S. Bon
  • R. R. R. Coelho
  • R. P. Nascimento
Article

Abstract

This study evaluated the production of cellulolytic enzymes by an Aspergillus fumigatus strain, isolated from sugar cane bagasse, according to its ability to grow on microcrystalline cellulose as the sole carbon source. The effect of the carbon source (brewer’s spent grain, sugarcane bagasse, and wheat bran) and of the nitrogen source (corn steep liquor and sodium nitrate) on cellulase production was studied using submerged and solid state cultivations at 30 °C. The highest levels of endoglucanase (CMCase) corresponded to 365 U L-1 and was obtained using sugarcane bagasse (1%) and corn steep liquor (1.2%) in submerged fermentation within 6 days of cultivation. This supernatant was used to run a sodium dodecyl sulfate polyacrylamide gel electrophoresis that showed six bands with endoglucanase activity. CMCase activity was higher at 65 °C and pH 2.0, indicating that this microorganism produces a thermophilic and acid endoglucanase. Solid state cultivation favored FPase production, that reached 47 U g-1 of dry substrate (wheat bran and sugarcane bagasse) within 3 days.

Keywords

Aspergillus fumigatus Thermophilic endoglucanase Acidophilic endoglucanase CMCase Agro-industrial by-products 

Notes

Acknowledgments

Authors are indebted to Marta de Sousa Ferreira for technical support and Fundação Oswaldo Cruz for identification of the fungal strain. This work was financially supported by CNPq and FINEP (project no 0106004700).

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Copyright information

© Humana Press 2008

Authors and Affiliations

  • A. L. Grigorevski-Lima
    • 1
  • F. N. M. Da Vinha
    • 1
  • D. T. Souza
    • 2
  • A. S. R. Bispo
    • 2
  • E. P. S. Bon
    • 3
  • R. R. R. Coelho
    • 1
  • R. P. Nascimento
    • 2
    • 4
  1. 1.Instituto de Microbiologia Prof. Paulo de Góes, Centro de Ciências da SaúdeUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Núcleo de Estudos em Microbiologia Aplicada (NEMA), Centro de Ciências Agrárias, Ambientais e BiológicasUniversidade Federal do Recôncavo da BahiaCruz das AlmasBrazil
  3. 3.Instituto de Química, Centro de TecnologiaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  4. 4.Universidade Federal do Recôncavo da BahiaCentro de Ciências Agrárias Ambientais e BiológicasCruz das AlmasBrazil

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