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Applied Biochemistry and Biotechnology

, Volume 153, Issue 1–3, pp 139–150 | Cite as

Lime Pretreatment of Sugarcane Bagasse for Bioethanol Production

  • Sarita C. Rabelo
  • Rubens Maciel Filho
  • Aline Carvalho CostaEmail author
Article

Abstract

The pretreatment of sugarcane bagasse with lime (calcium hydroxide) is evaluated. The effect of lime pretreatment on digestibility was studied through analyses using central composite design (response surface), considering pretreatment time, temperature, and lime loading as factors. The responses evaluated were the yield of glucose from pretreated bagasse after enzymatic hydrolysis. Experiments were performed using the bagasse as it comes from an alcohol/sugar factory (non-screened bagasse) and bagasse in the size range from 0.248 to 1.397 mm (screened bagasse) (12-60 mesh). It was observed that the particle size presented influence in the release of fermentable sugars after enzymatic hydrolysis using low loading of cellulase and β-glucosidase (3.5 FPU/g dry pretreated biomass and 1.0 IU/g dry pretreated biomass, respectively).

Keywords

Lignocellulose materials Sugarcane bagasse Pretreatment Lime Enzymatic hydrolysis Statistical analysis 

Notes

Acknowledgments

The authors acknowledge Fapesp for financial support.

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Copyright information

© Humana Press 2008

Authors and Affiliations

  • Sarita C. Rabelo
    • 1
  • Rubens Maciel Filho
    • 1
  • Aline Carvalho Costa
    • 1
    Email author
  1. 1.School of Chemical EngineeringUniversity of Campinas, UNICAMPCampinasBrazil

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