Bacterial cellulose production by Gluconacetobacter sp. PKY5 in a rotary biofilm contactor
A rotary biofilm contactor (RBC) inoculated with Gluconacetobacter sp. RKY5 was used as a bioreactor for improved bacterial cellulose production. The optimal number of disk for bacterial cellulose production was found to be eight, at which bacterial cellulose and cell concentrations were 5.52 and 4.98 g/L. When the aeration rate was maintained at 1.25 vvm, bacterial cellulose and cell concentrations were maximized (5.67 and 5.25 g/L, respectively). The optimal rotation speed of impeller in RBC was 15 rpm. When the culture pH in RBC was not controlled during fermentation, the maximal amount of bacterial cellulose (5.53 g/L) and cells (4.91 g/L) was obtained. Under the optimized culture conditions, bacterial cellulose and cell concentrations in RBC reached to 6.17 and 5.58 g/L, respectively.
Index EntriesBacterial cellulose bioreactor fermentation Gluconacetobacter optimization rotary biofilm contactor
- 3.Ralph, J. F., Fessenden, J. S., and Marshall, W. L. (1998), Organic Chemistry, 6th ed. Brooks/Cole Publishing Company, USA, 952p.Google Scholar
- 4.Richmond, P. A. (1991), In: Biosynthesis and Biodegradation of Cellulose, Haigler, C. H. and Weimer, P. J., (eds.), Marcel Dekker, New York, pp. 5–23.Google Scholar
- 10.Valla, S. and Kjosbakken, J. (1981), J. Gen. Microbiol. 128, 1401–1418.Google Scholar
- 12.Schramm, M. and Hestrin, S. (1954), Biochem. J. 56, 163–166.Google Scholar