Applied Biochemistry and Biotechnology

, Volume 137, Issue 1–12, pp 195–204

Thermoascus aurantiacus CBHI/Cel7A production in Trichoderma reesei on alternative carbon sources

  • Zsuzsa Benkő
  • Eszter Drahos
  • Zsolt Szengyel
  • Terhi Puranen
  • Jari Vehmaanperä
  • Kati Réczey
Session 1A: Enzyme Catalysis And Engineering

DOI: 10.1007/s12010-007-9051-5

Cite this article as:
Benkő, Z., Drahos, E., Szengyel, Z. et al. Appl Biochem Biotechnol (2007) 137: 195. doi:10.1007/s12010-007-9051-5

Abstract

To develop functional enzymes in cellulose hydrolysis at or above 70°C the cellobiohydrolase (CBHI/Cel7A) of Thermoascus aurantiacus was cloned and expressed in Trichoderma reesei Rut-C30 under the strong cbh1 promoter. Cellulase production of the parental strain and the novel strain (RF6026) was examined in submerged fermentation experiments using various carbon sources, which were lactose, Solka Floc 200 cellulose powder, and steam pretreated corn stover. An industrially feasible production medium was used containing only distiller’s spent grain, KH2PO4, and (NH4)2SO4. Enzyme production was followed by measurements of protein concentration, total cellulase enzyme activity (filter paper activity), β-glucosidase activity, CBHI activity, and endogenase I (EGI) activity. The Thermoascus CBHI/Cel7A activity was taken as an indication of the heterologous gene expression under the cbh1 promoter.

Index Entries

Cellulose fermentor lactose shake flask Solka Floc 200 steam pretreated corn stover 

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Copyright information

© Humana Press Inc 2007

Authors and Affiliations

  • Zsuzsa Benkő
    • 1
  • Eszter Drahos
    • 1
  • Zsolt Szengyel
    • 1
  • Terhi Puranen
    • 2
  • Jari Vehmaanperä
    • 2
  • Kati Réczey
    • 1
  1. 1.Department of Agricultural Chemical TechnologyBudapest University of Technology and EconomicsBudapestHungary
  2. 2.ROAL OyRajamäkiFinland

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