Applied Biochemistry and Biotechnology

, Volume 137, Issue 1–12, pp 141–153 | Cite as

Enzymatic hydrolysis optimization to ethanol production by simultaneous saccharification and fermentation

  • Mariana Peñuela Vásquez
  • Juliana Nascimento C. da Silva
  • Maurício Bezerra de Souza
  • Nei Pereira
Session 1A: Enzyme Catalysis And Engineering


There is tremendous interest in using agro-industrial wastes, such as cellulignin, as starting materials for the production of fuels and chemicals. Cellulignin are the solids, which result from the acid hydrolysis of the sugarcane bagasse. The objective of this work was to optimize the enzymatic hydrolysis of the cellulose fraction of cellulignin, and to study its fermentation to ethanol using Saccharomyces cerevisiae. Cellulose conversion was optimized using response surface methods with pH, enzyme loading, solid percentage, and temperature as factor variables. The optimum conditions that maximized the conversion of cellulose to glucose, calculated from the initial dried weight of pretreated cellulignin, (43°C, 2%, and 24.4 FPU/g of pretreated cellulignin) such as the glucose concentration (47°C, 10%, and 25.6 FPU/g of pretreated cellulignin) were found. The desirability function was used to find conditions that optimize both, conversion to glucose and glucose concentration (47°C, 10%, and 25.9 FPU/g of pretreated cellulignin). The resulting enzymatic hydrolyzate was fermented yielding a final ethanol concentration of 30.0 g/L, in only 10 h, and reaching a volumetric productivity of 3.0 g/L·h, which is close to the values obtained in the conventional ethanol fermentation of sugar cane juice (5.0–8.0 g/L·h) in Brazil.

Index Entries

Cellulignin enzymatic hydrolysis ethanol production sugarcane bagasse cellulases simultaneous saccharification saccharomyces cerevisiae 


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Copyright information

© Humana Press Inc 2007

Authors and Affiliations

  • Mariana Peñuela Vásquez
    • 1
    • 2
  • Juliana Nascimento C. da Silva
    • 1
  • Maurício Bezerra de Souza
    • 1
  • Nei Pereira
    • 1
  1. 1.Centro de Tecnologia—Bloco EEscola de Química—Universidade Federal do Rio de JaneiroRio de Janeiro-RJBrasil
  2. 2.Departamento de Ingeniería QuímicaUniversidad de AntioquiaColombia

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