Applied Biochemistry and Biotechnology

, Volume 137, Issue 1–12, pp 17–25 | Cite as

Enzymatic microreactors for the determination of ethanol by an automatic sequential injection analysis system

  • Eliana M. AlhadeffEmail author
  • Andrea M. Salgado
  • Oriol Cos
  • Nei Pereira
  • BelkisValdman
  • Francisco Valero
Session 1A: Enzyme Catalysis And Engineering


A sequential injection analysis system with two enzymatic microreactors for the determination of ethanol has been designed. Alcohol oxidase and horseradish peroxidase were separately immobilized on glass aminopropyl beads, and packed in 0.91-mL volume microreactors, working in line with the sequential injection analysis system. A stop flow of 120 s was selected for a linear ethanol range of 0.005–0.04 g/L ± 0.6% relative standard deviation with a throughput of seven analyses per hour. The system was applied to measure ethanol concentrations in samples of distilled and nondistilled alcoholic beverages, and of alcoholic fermentation with good performance and no significant difference compared with other analytical procedures (gas chromatography and high-performance liquid chromatography).

Index Entries

Alcohol oxidase ethanol horseradish peroxidase immobilized enzymes sequential injection analysis biosensors 


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Copyright information

© Humana Press Inc 2007

Authors and Affiliations

  • Eliana M. Alhadeff
    • 1
    Email author
  • Andrea M. Salgado
    • 1
  • Oriol Cos
    • 2
  • Nei Pereira
    • 1
  • BelkisValdman
    • 1
  • Francisco Valero
    • 2
  1. 1.Escola de Química, Centro de TecnologiaUniversidade Federal do Rio de Janeiro, Ilha do Fundão, Cidade Universitária, CEP: 21.949-900Rio de JaneiroBrasil
  2. 2.Department d’Enginyeria Química, Escola Tècnica Superior d’EnginyeriaUniversitat Autònoma de BarcelonaBellaterra, BarcelonaSpain

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