Integrated Biosensor Systems for Ethanol Analysis
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Different integrated systems with a bi-enzymatic biosensor, working with two different methods for ethanol detection—flow injection analysis (FIA) or sequential injection analysis (SIA)—were developed and applied for ethanol extracted from gasohol mixtures, as well as for samples of alcoholic beverages and fermentation medium. A detection range of 0.05–1.5 g ethanol/l, with a correlation coefficient of 0.9909, has been reached when using FIA system, working with only one microreactor packed with immobilized alcohol oxidase and injecting free horseradish peroxidase. When using both enzymes, immobilized separately in two microreactors, the detection ranges obtained varied from 0.001 to 0.066 g ethanol/l, without on-line dilution to 0.010–0.047 g ethanol/l when a 1:7,000 dilution ratio was employed, reaching correlation coefficients of 0.9897 and 0.9992, respectively. For the integrated biosensor SIA system with the stop–flow technique, the linear range was 0.005–0.04 g/l, with a correlation coefficient of 0.9922.
KeywordsFlow injection analysis Sequential injection analysis Ethanol Alcohol oxidase Horseradish peroxidase Biosensor
We acknowledge the support received from the BI-EURAM Project of the Alpha Program-EU. We also would like to thank Toyobo of Brazil for gently donating the horseradish peroxidase. We also acknowledge LABCOM-EQ/UFRJ and Refinaria de Petróleos Manguinhos, for donating, respectively, the different samples of gasohol mixture and gasoline A.
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