Applied Biochemistry and Biotechnology

, Volume 147, Issue 1–3, pp 33–45 | Cite as

Oxygen-controlled Biosurfactant Production in a Bench Scale Bioreactor

  • Frederico de Araujo Kronemberger
  • Lidia Maria Melo Santa Anna
  • Ana Carolina Loureiro Brito Fernandes
  • Reginaldo Ramos de Menezes
  • Cristiano Piacsek Borges
  • Denise Maria Guimarães Freire
Article
First Online:
Received:
Accepted:

Abstract

Rhamnolipids have been pointed out as promising biosurfactants. The most studied microorganisms for the aerobic production of these molecules are the bacteria of the genus Pseudomonas. The aim of this work was to produce a rhamnolipid-type biosurfactant in a bench-scale bioreactor by one strain of Pseudomonas aeruginosa isolated from oil environments. To study the microorganism growth and production dependency on oxygen, a nondispersive oxygenation device was developed, and a programmable logic controller (PLC) was used to set the dissolved oxygen (DO) concentration. Using the data stored in a computer and the predetermined characteristics of the oxygenation device, it was possible to evaluate the oxygen uptake rate (OUR) and the specific OUR (SOUR) of this microorganism. These rates, obtained for some different DO concentrations, were then compared to the bacterial growth, to the carbon source consumption, and to the rhamnolipid and other virulence factors production. The SOUR presented an initial value of about 60.0 mgO2/gDW h. Then, when the exponential growth phase begins, there is a rise in this rate. After that, the SOUR reduces to about 20.0 mgO2/gDW h. The carbon source consumption is linear during the whole process.

Keywords

Pseudomonas aeruginosa Biosurfactant Oxygenation Rhamnolipid Bioreactor 
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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Frederico de Araujo Kronemberger
    • 1
  • Lidia Maria Melo Santa Anna
    • 2
  • Ana Carolina Loureiro Brito Fernandes
    • 3
  • Reginaldo Ramos de Menezes
    • 3
  • Cristiano Piacsek Borges
    • 1
  • Denise Maria Guimarães Freire
    • 3
  1. 1.Instituto Alberto Luiz Coimbra de Pós Graduação e Pesquisa de Engenharia, Programa de Engenharia QuímicaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil
  2. 2.Gerência de Biotecnologia e Tratamentos AmbientaisCentro de Pesquisa e Desenvolvimento Leopoldo Américo Miguêz de MelloRio de JaneiroBrazil
  3. 3.Departamento de Bioquímica, Instituto de QuímicaUniversidade Federal do Rio de JaneiroRio de JaneiroBrazil

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