Applied Biochemistry and Biotechnology

, Volume 143, Issue 3, pp 236–243

An Organic Soluble Lipase for Water-Free Synthesis of Biodiesel

  • Xueyan Zhao
  • Bilal El-Zahab
  • Ryan Brosnahan
  • Justin Perry
  • Ping Wang
Article

Abstract

Lipase AK was modified with short alkyl chains to form a highly organic soluble enzyme and was used to catalyze the synthesis of biodiesel from soybean oil in organic media. The effects of several key factors including water content, temperature, and solvent were examined for the solubilized enzyme in comparison with several other commercially available lipases. Whereas native lipases showed no activity in the absence of water, the organic soluble lipase demonstrated reaction rates of up to 33 g-product/g-enzyme h. The biocatalyst remains soluble in the biodiesel product, and therefore, there is no need to be removed because it is expected to be burned along with the diesel in combustion engines. This provides a promising one-pot mix-and-use strategy for biodiesel production.

Keywords

Biodiesel Transesterification Nonaqueous biocatalysis Lipases Enzymes 

References

  1. 1.
    Directive 2003/30/EC of The European Parliament and of the Council on the Promotion of the Use of Biofuels or Other Renewable Fuels for Transport. Official Journal of the European Union, May 17, 2003.Google Scholar
  2. 2.
    National Biodiesel Board News Release. Nov 14, 2006.Google Scholar
  3. 3.
    Al-Zuhair, S. (2005). Biotechnology Progress, 21, 1442–1448.CrossRefGoogle Scholar
  4. 4.
    Du, W., Xu, Y.-Y., Liu, D.-H., & Li, Z.-B. (2005). Journal of Molecular Catalysis, B, Enzymatic, 37, 68–71.CrossRefGoogle Scholar
  5. 5.
    Salis, A., Pinna, M., Monduzzi, M., & Solinas, V. (2005). Journal of Biotechnology, 119, 291–299.CrossRefGoogle Scholar
  6. 6.
    Noureddini, H., Gao, X., & Philkana, R. S. (2005). Bioresource Technology, 96, 769–777.CrossRefGoogle Scholar
  7. 7.
    Samukawa, T., Kaieda, M., Matsumoto, T., Ban, K., Konda, A., & Shimada, Y., et al. (2000). Journal of Bioscience and Bioengineering, 90, 180–183.Google Scholar
  8. 8.
    Du, W., Xu, Y.-Y., Zeng, J., & Liu, D.-H. (2004). Biotechnology and Applied Biochemistry, 40, 187–190.CrossRefGoogle Scholar
  9. 9.
    Kaieda, M., Samukawa, T., Kondo, A., & Fukuda, H. (2001). Journal of Bioscience and Bioengineering, 91, 12–15.CrossRefGoogle Scholar
  10. 10.
    Oda, M., Kaieda, M., Hama, S., Yamaji, H., Kondo, A., & Izumoto, E., et al. (2005). Biochemical Engineering Journal, 23, 45–51.CrossRefGoogle Scholar
  11. 11.
    National Biodiesel Board—Specification for Biodiesel (B100). May 1999.Google Scholar
  12. 12.
    Klibanov, A. M. (2001). Nature, 409, 241–246.CrossRefGoogle Scholar
  13. 13.
    Ohya, Y., Sugitou, T., & Ouchi, T. (1995). Pure and Applied Chemistry, A32, 179–190.Google Scholar
  14. 14.
    Wang, P., Woodward, C. A., & Kaufman, E. N. (1999). Biotechnology and Bioengineering, 64, 290–197.CrossRefGoogle Scholar
  15. 15.
    Distel, K. A., Zhu, G., & Wang, P. (2005). Bioresource Technology, 96, 617–623.CrossRefGoogle Scholar
  16. 16.
    Homandberg, G. A., Mattis, J. A., & Laskowski, M., Jr. (1978). Biochemistry, 17(24), 5220–5227.CrossRefGoogle Scholar
  17. 17.
    Zaks, A., & Klibanov, A. M. (1984). Science, 224(4654), 1249–1251.CrossRefGoogle Scholar
  18. 18.
    Lara, P. V., & Park, E. Y. (2004). Enzyme and Microbial Technology, 34, 270–277.CrossRefGoogle Scholar
  19. 19.
    Chang, H.-M., Liao, H.-F., Lee, C.-C., & Shieh, C.-J. (2005). Journal of Chemical Technology and Biotechnology, 80, 307–312.CrossRefGoogle Scholar
  20. 20.
    Xu, Y., Du, W., Zeng, J., & Liu, D. (2004). Biocatalysis and Biotransformation, 22, 45–48.CrossRefGoogle Scholar

Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  • Xueyan Zhao
    • 1
    • 2
  • Bilal El-Zahab
    • 2
  • Ryan Brosnahan
    • 2
    • 3
  • Justin Perry
    • 2
    • 3
  • Ping Wang
    • 1
    • 2
  1. 1.Department of Bioproducts and Biosystems EngineeringBiotechnology Institute, University of MinnesotaSt. PaulUSA
  2. 2.Department of Chemical EngineeringUniversity of AkronAkronUSA
  3. 3.Firestone High SchoolAkronUSA

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