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Development of a New Bioprocess for Production of 1,3-propanediol I.: Modeling of Glycerol Bioconversion to 1,3-propanediol with Klebsiella pneumoniae Enzymes

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Abstract

Glycerol is a renewable resource for it is formed as a byproduct during biodiesel production. Because of its large volume production, it seems to be a good idea to develop a technology that converts this waste into products of high value, for example, to 1,3-propanediol (1,3-PD). We suggested an enzymatic bioconversion in a membrane reactor in which the NAD coenzyme can be regenerated, and three key enzymes are retained by a 10-kDa ultrafilter membrane. Unfortunately, some byproducts also formed during successful glycerol to 1,3-PD bioconversion runs, as we used crude enzyme solution of Klebsiella pneumoniae. To study the possibilities to avoid this byproduct formation, we built a mathematical description of this system. The model was also used for simulation bioconversions of high glycerol concentration with and without elimination of byproduct formation and of continuous operation.

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Abbreviations

1,3-PD:

1,3-propanediol

DHA:

1,3-dihydroxyacetone

GDH:

glycerol dehydrogenase enzyme

GDHt:

glycerol dehydratase enzyme

PDOR:

1,3-propanediol oxydoreductase

DHAK:

dihydroxyacetone kinase

TPI:

triose-phosphate isomerase

GAPD:

glyceraldehyde-3-phosphate dehydrogenase

PGK:

phosphoglycerate kinase

PGM:

phosphoglycerate mutase

PPH:

phosphopyruvate hydratase

PK:

pyruvate kinase

PS:

pyruvate synthase

PAT:

phosphate acetyltransferase

PTA:

phospholipid-translocating ATPase

LDH:

lactate-dehydrogenase

LMO:

lactate 2-monooxygenase

BB:

“black-box”

Clha:

chloro-3-hydroxyacetone

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Acknowledgment

We are thankful to OTKA T032015 and NKFP-3/A/0035/2002 for the financial support of our work.

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Authors and Affiliations

  1. Department of Agricultural Chemical Technology, Budapest University of Technology and Economics, Budapest, Hungary

    Áron Németh & Béla Sevella

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  1. Áron Németh
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  2. Béla Sevella
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Correspondence to Béla Sevella.

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Németh, Á., Sevella, B. Development of a New Bioprocess for Production of 1,3-propanediol I.: Modeling of Glycerol Bioconversion to 1,3-propanediol with Klebsiella pneumoniae Enzymes. Appl Biochem Biotechnol 144, 47–58 (2008). https://doi.org/10.1007/s12010-007-0040-5

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  • DOI: https://doi.org/10.1007/s12010-007-0040-5

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