Applied Biochemistry and Biotechnology

, Volume 144, Issue 1, pp 47–58 | Cite as

Development of a New Bioprocess for Production of 1,3-propanediol I.: Modeling of Glycerol Bioconversion to 1,3-propanediol with Klebsiella pneumoniae Enzymes

Article

Abstract

Glycerol is a renewable resource for it is formed as a byproduct during biodiesel production. Because of its large volume production, it seems to be a good idea to develop a technology that converts this waste into products of high value, for example, to 1,3-propanediol (1,3-PD). We suggested an enzymatic bioconversion in a membrane reactor in which the NAD coenzyme can be regenerated, and three key enzymes are retained by a 10-kDa ultrafilter membrane. Unfortunately, some byproducts also formed during successful glycerol to 1,3-PD bioconversion runs, as we used crude enzyme solution of Klebsiella pneumoniae. To study the possibilities to avoid this byproduct formation, we built a mathematical description of this system. The model was also used for simulation bioconversions of high glycerol concentration with and without elimination of byproduct formation and of continuous operation.

Keywords

1,3-Propanediol Klebsiella pneumoniae Enzymatic bioconversion Modelling 

Abbreviations

1,3-PD

1,3-propanediol

DHA

1,3-dihydroxyacetone

GDH

glycerol dehydrogenase enzyme

GDHt

glycerol dehydratase enzyme

PDOR

1,3-propanediol oxydoreductase

DHAK

dihydroxyacetone kinase

TPI

triose-phosphate isomerase

GAPD

glyceraldehyde-3-phosphate dehydrogenase

PGK

phosphoglycerate kinase

PGM

phosphoglycerate mutase

PPH

phosphopyruvate hydratase

PK

pyruvate kinase

PS

pyruvate synthase

PAT

phosphate acetyltransferase

PTA

phospholipid-translocating ATPase

LDH

lactate-dehydrogenase

LMO

lactate 2-monooxygenase

BB

“black-box”

Clha

chloro-3-hydroxyacetone

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Copyright information

© Humana Press Inc. 2007

Authors and Affiliations

  1. 1.Department of Agricultural Chemical TechnologyBudapest University of Technology and EconomicsBudapestHungary

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