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Applied Biochemistry and Biotechnology

, Volume 142, Issue 2, pp 105–124 | Cite as

Expression of Recombinant Proteins in Pichia Pastoris

  • Pingzuo Li
  • Anukanth Anumanthan
  • Xiu-Gong Gao
  • Kuppusamy Ilangovan
  • Vincent V. Suzara
  • Nejat Düzgüneş
  • V. RenugopalakrishnanEmail author
Article

Abstract

Pichia pastoris has been used extensively and successfully to express recombinant proteins. In this review, we summarize the elements required for expressing heterologous proteins, and discuss various factors in applying this system for protein expression. These elements include vectors, host strains, heterologous gene integration into the genome, secretion factors, and the glycosylation profile. In particular, we discuss and evaluate the recent progress in optimizing the fermentation process to improve the yield and stability of expressed proteins. Optimization can be achieved by controlling the medium composition, pH, temperature, and dissolved oxygen, as well as by methanol induction and feed mode.

Keywords

Pichia pastoris Protein expression Methanol induction Dissolved oxygen Gene integration Alcohol oxidase promoter AOX1 

Notes

Acknowledgments

We thank Dr. Geoff Lin-Cereghino (Department of Biology, University of the Pacific) for helpful comments on the manuscript, and Mr. Neal Johnson (School of Dentistry, University of the Pacific) for preparing the figures. The research described here was supported by US AFOSR, NSF, the Wallace Coulter Foundation, and Harvard Medical School.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Pingzuo Li
    • 1
    • 2
  • Anukanth Anumanthan
    • 3
  • Xiu-Gong Gao
    • 4
  • Kuppusamy Ilangovan
    • 5
  • Vincent V. Suzara
    • 6
  • Nejat Düzgüneş
    • 6
  • V. Renugopalakrishnan
    • 2
    • 7
    • 8
    Email author
  1. 1.Shanghai Research Center of BiotechnologyChinese Academy of SciencesShanghaiPeople’s Republic of China
  2. 2.Children’s HospitalHarvard Medical SchoolBostonUSA
  3. 3.Department of Biological Chemistry and Molecular PharmacologyHarvard Medical SchoolBostonUSA
  4. 4.Laboratory of Cell BiologyNational Cancer Institute, National Institutes of HealthBethesdaUSA
  5. 5.Centro de Investigacion en Calidad AmbientalTecnologico de Monterrey, Campus Estado de MéxicoMonterreyMexico
  6. 6.Department of Microbiology, Arthur A. Dugoni School of DentistryUniversity of the PacificSan FranciscoUSA
  7. 7.Bionanotechnology GroupFlorida International UniversityMiamiUSA
  8. 8.Biophotovoltaic Group, Division of Bioengineering and Department of Mechanical Engineering, NUS Nanotechnology Initiative (NUSNNI)National University of SingaporeSingaporeSingapore

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