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Clinical Orthopaedics and Related Research®

, Volume 473, Issue 6, pp 1913–1919 | Cite as

Experimental Disc Herniation in the Rat Causes Downregulation of Serotonin Receptor 2c in a TNF-dependent Manner

  • Daniel Jonsson
  • Oscar Finskas
  • Yuki Fujioka
  • Anders Ståhlberg
  • Kjell OlmarkerEmail author
Symposium: Current Approaches to the Management of Lumbar Disc Herniation

Abstract

Background

During recent decades, the knowledge of the pathophysiology of disc herniation and sciatica has drastically improved. What previously was considered a strict biomechanical process is now considered a more complex interaction between leaked nucleus pulposus and the tissue in the spinal canal. An inflammatory reaction, with tumor necrosis factor (TNF) playing an essential role, has been demonstrated. However, the exact mechanisms of the pathophysiology of disc herniation remain unknown.

Questions/purposes

In this study we use an animal model to investigate (1) if and/or how experimental disc herniation affects gene expression in the early phase (24 hours postsurgery) in the dorsal root ganglion; and (2) if TNF inhibition can reduce any observed changes.

Methods

A rat model of disc herniation was used. Twenty rats were evenly divided into four groups: naïve, sham, disc herniation, and disc herniation with TNF inhibition. The dorsal root ganglion of the affected nerve root was harvested 24 hours after surgery and analyzed with a TaqMan Low Density Array® quantitative polymerase chain reaction assay. Gene expression levels in sham were compared with disc herniation to assess question 1 and disc herniation to disc herniation with TNF inhibition to assess question 2.

Results

Experimental disc herniation caused a decrease in the expression of the serotonin receptor 2c gene (p = 0.022). TNF inhibition was found to reduce the observed decrease in expression of serotonin receptor 2c (p = 0.037).

Conclusions

Our results suggest that a decrease in the expression of the serotonin receptor 2c gene may contribute to the pathophysiology of disc herniation. Further research on its involvement is warranted.

Clinical Relevance

This pilot study gives a brief insight into cellular changes that may contribute to the pathophysiology of disc herniation. This knowledge may contribute to the development of more and better treatment options for patients with disc herniation and sciatica.

Keywords

Dorsal Root Ganglion Nucleus Pulposus Disc Herniation Tumor Necrosis Factor Inhibition Disc Puncture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The Association of Bone and Joint Surgeons® 2014

Authors and Affiliations

  • Daniel Jonsson
    • 1
  • Oscar Finskas
    • 1
  • Yuki Fujioka
    • 1
    • 2
  • Anders Ståhlberg
    • 3
  • Kjell Olmarker
    • 1
    Email author
  1. 1.Musculoskeletal Research, Department of Medical Chemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska AcademyUniversity of GothenburgGothenburgSweden
  2. 2.Department of Orthopaedic Surgery, Programs for Applied Biomedicine, Graduate School of Biomedical SciencesHiroshima UniversityHiroshimaJapan
  3. 3.Sahlgrenska Cancer Center, Department of Pathology, Sahlgrenska AcademyUniversity of GothenburgGothenburgSweden

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