Clinical Orthopaedics and Related Research®

, Volume 468, Issue 11, pp 3112–3120

Increased Chondrocyte Death after Steroid and Local Anesthetic Combination

  • Boglárka Farkas
  • Krisztián Kvell
  • Tamás Czömpöly
  • Tamás Illés
  • Tamás Bárdos
Basic Research

DOI: 10.1007/s11999-010-1443-0

Cite this article as:
Farkas, B., Kvell, K., Czömpöly, T. et al. Clin Orthop Relat Res (2010) 468: 3112. doi:10.1007/s11999-010-1443-0

Abstract

Background

Hyaline articular cartilage has limited repair and regeneration capacity. Intraarticular administration of glucocorticoid and local anesthetic injections play an important role in the therapy of osteoarthritis. Glucocorticoids and anesthetics reportedly enhance apoptosis in chondrocytes, but effects of the combined use of glucocorticoids and local anesthetics are unknown.

Questions/purposes

We asked whether glucocorticoid and local anesthetic agents combined had any synergistic effects on chondrocyte apoptosis.

Methods

Cell viability and apoptosis/necrosis assessment of human articular chondrocytes were performed in vitro (chondrocyte cell cultures) and ex vivo (osteochondral specimens) using flow cytometry and TUNEL analysis, respectively.

Results

Glucocorticoids and local anesthetics induce apoptosis in chondrocytes at various rates. When used in combination, the percentage of dead chondrocytes was increased in in vitro chondrocyte cell cultures and osteochondral ex vivo specimens.

Conclusions

We observed a time-dependent decrease in chondrocyte viability after concurrent steroid and local anesthetic exposure.

Clinical Relevance

The combination of glucocorticoids and local anesthetics has an adverse effect on articular chondrocytes, and it raises a question regarding whether concomitant administration should be used in treating osteoarthritis.

Copyright information

© The Association of Bone and Joint Surgeons® 2010

Authors and Affiliations

  • Boglárka Farkas
    • 1
    • 2
  • Krisztián Kvell
    • 3
  • Tamás Czömpöly
    • 3
  • Tamás Illés
    • 1
  • Tamás Bárdos
    • 1
  1. 1.Department of OrthopaedicsUniversity of PécsPécsHungary
  2. 2.Department of AnatomyUniversity of PécsPécsHungary
  3. 3.Department of Immunology and BiotechnologyUniversity of PécsPécsHungary

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