Successful Spinal Fusion by E. coli-derived BMP-2-adsorbed Porous β-TCP Granules: A Pilot Study
Bone morphogenetic proteins (BMPs) were originally identified as osteoinductive proteins. With cloning of BMP genes, studies of BMPs and their clinical application have advanced. However, with increasing clinical applications, drug delivery systems and production costs have become more important issues. To address these issues, we asked whether E. coli-derived rhBMP-2 (E-BMP-2)-adsorbed porous β-TCP granules could achieve posterolateral lumbar fusion in a rabbit model similar to autogenous bone grafts. Lumbar spinal fusion masses were evaluated by 3-D computed tomography, mechanical testing, and histological analyses 8 weeks after surgery. By these measures E-BMP-2-adsorbed β-TCP granules achieved lumbar spinal fusion in dose-dependent fashion in a rabbit model as well as autogenous bone graft. Our preliminary findings suggest E-BMP-2-adsorbed porous β-TCP could be a novel, effective alternative to autogenous bone grafting for generating new bone and promoting regenerative repair of bone, and potentially utilizable in the clinical setting for treating spinal disorders.
KeywordsSpinal Fusion Transverse Process Autogenous Bone Autogenous Bone Graft Lumbar Spinal Fusion
We thank Drs. A. Suzuki, K. Yano, T. Matsumoto, H. Yasuda, K. Sugama, H. Irie, and A. Yamada, W. Fukushima, M Fukui, and also Ms. A. Inagaki, Ms. K. Hata, Ms. K. Kamei, and Ms. Y. Hanamoto for technical and statistical assistance. E-BMP-2 was produced and kindly provided by Dr. W. Sebald (Würzburg, Germany) and donated to us through Osteopharma Inc. (Osaka, Japan). The β-TCP granules were donated by HOYA Corp. (Tokyo, Japan).
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