Clinical Orthopaedics and Related Research®

, Volume 467, Issue 8, pp 2159–2167

Different Differentiation of Stroma Cells from Patients with Osteonecrosis: A Pilot Study

Original Article Research

DOI: 10.1007/s11999-009-0803-0

Cite this article as:
Yeh, CH., Chang, JK., Ho, ML. et al. Clin Orthop Relat Res (2009) 467: 2159. doi:10.1007/s11999-009-0803-0


Osteonecrosis (ON) is commonly caused by high doses of glucocorticoids or ethanol intake. We previously reported suppression of BMP-2 by dexamethasone was more pronounced and enhancement by lovastatin was less pronounced in bone marrow stromal cells (BMSCs) from patients with ON than from patients without ON. We therefore presumed the BMSCs might be dysfunctional in patients with ON and performed this pilot study. We obtained BMSCs from 10 patients with ethanol-induced ON, 10 patients with glucocorticoid-induced ON, and 12 patients without ON as control subjects, checking third passage cells for osteogenic and adipogenic gene expression and differentiation ability. BMSCs from patients with glucocorticoid-induced ON possessed less osteogenic gene expression and less osteogenic differentiation, whereas BMSCs from patients with ethanol-induced ON possessed more adipogenic gene expression and more adipogenic differentiation. Dysfunction of BMSCs may be one of the causes of ON, with differing dysfunction in ethanol-induced ON and glucocorticoid-induced ON. Glucocorticoids may possess more of a suppressive effect on osteogenesis than ethanol, whereas ethanol may possess a more potent adipogenic effect than glucocorticoids on BMSCs.

Copyright information

© The Association of Bone and Joint Surgeons 2009

Authors and Affiliations

  1. 1.Department of Physiology, College of MedicineKaohsiung Medical UniversityKaohsiungTaiwan
  2. 2.Orthopaedic Research CenterKaohsiung Medical UniversityKaohsiungTaiwan
  3. 3.Graduate Institute of MedicineKaohsiung Medical UniversityKaohsiungTaiwan
  4. 4.Center for Cervical Cancer Prevention, Department of ResearchBuddhist Tzu Chi General HospitalHualienTaiwan
  5. 5.Department of Obstetrics and GynecologyBuddhist Tzu Chi General HospitalHualienTaiwan
  6. 6.Department of Orthopaedics, College of MedicineKaohsiung Medical UniversityKaohsiungTaiwan
  7. 7.Department of OrthopaedicsKaohsiung Medical University Hospital, Kaohsiung Medical UniversityKaohsiung CityTaiwan
  8. 8.Department of Orthopaedic SurgeryUniversity of VirginiaCharlottesvilleUSA
  9. 9.Department of Orthopaedic SurgeryNational Cheng-Kung UniversityKaohsiung CityTaiwan

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