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Clinical Orthopaedics and Related Research

, Volume 466, Issue 8, pp 1938–1948 | Cite as

Mechanoactive Scaffold Induces Tendon Remodeling and Expression of Fibrocartilage Markers

  • Jeffrey P. Spalazzi
  • Moira C. Vyner
  • Matthew T. Jacobs
  • Kristen L. Moffat
  • Helen H. LuEmail author
Symposium: New Approaches to Allograft Transplantation

Abstract

Biological fixation of soft tissue-based grafts for anterior cruciate ligament (ACL) reconstruction poses a major clinical challenge. The ACL integrates with subchondral bone through a fibrocartilage enthesis, which serves to minimize stress concentrations and enables load transfer between two distinct tissue types. Functional integration thus requires the reestablishment of this fibrocartilage interface on reconstructed ACL grafts. We designed and characterized a novel mechanoactive scaffold based on a composite of poly-α-hydroxyester nanofibers and sintered microspheres; we then used the scaffold to test the hypothesis that scaffold-induced compression of tendon grafts would result in matrix remodeling and the expression of fibrocartilage interface-related markers. Histology coupled with confocal microscopy and biochemical assays were used to evaluate the effects of scaffold-induced compression on tendon matrix collagen distribution, cellularity, proteoglycan content, and gene expression over a 2-week period. Scaffold contraction resulted in over 15% compression of the patellar tendon graft and upregulated the expression of fibrocartilage-related markers such as Type II collagen, aggrecan, and transforming growth factor-β3 (TGF-β3). Additionally, proteoglycan content was higher in the compressed tendon group after 1 day. The data suggest the potential of a mechanoactive scaffold to promote the formation of an anatomic fibrocartilage enthesis on tendon-based ACL reconstruction grafts.

Keywords

Anterior Cruciate Ligament Anterior Cruciate Ligament Reconstruction Tendon Graft Anterior Cruciate Ligament Graft Hamstring Tendon Graft 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We thank Ms. Ciji Rich of the Biomaterials and Interface Tissue Engineering Laboratory at Columbia University for assistance in quantifying nanofiber mesh contraction, as well as Dr. X. Edward Guo of Columbia University for the use of the polarized light microscope for imaging collagen organization.

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Copyright information

© The Association of Bone and Joint Surgeons 2008

Authors and Affiliations

  • Jeffrey P. Spalazzi
    • 1
  • Moira C. Vyner
    • 1
  • Matthew T. Jacobs
    • 1
  • Kristen L. Moffat
    • 1
  • Helen H. Lu
    • 1
    • 2
    Email author
  1. 1.Department of Biomedical Engineering, Biomaterials and Interface Tissue Engineering LaboratoryColumbia UniversityNew YorkUSA
  2. 2.College of Dental MedicineColumbia UniversityNew YorkUSA

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