Clinical Orthopaedics and Related Research

, Volume 466, Issue 8, pp 1912–1920 | Cite as

Shaped, Stratified, Scaffold-free Grafts for Articular Cartilage Defects

  • EunHee Han
  • Won C. Bae
  • Nancy D. Hsieh-Bonassera
  • Van W. Wong
  • Barbara L. Schumacher
  • Simon Görtz
  • Koichi Masuda
  • William D. Bugbee
  • Robert L. Sah
Symposium: New Approaches to Allograft Transplantation

Abstract

One goal of treatment for large articular cartilage defects is to restore the anatomic contour of the joint with tissue having a structure similar to native cartilage. Shaped and stratified cartilaginous tissue may be fabricated into a suitable graft to achieve such restoration. We asked if scaffold-free cartilaginous constructs, anatomically shaped and targeting spherically-shaped hips, can be created using a molding technique and if biomimetic stratification of the shaped constructs can be achieved with appropriate superficial and middle/deep zone chondrocyte subpopulations. The shaped, scaffold-free constructs were formed from the alginate-released bovine calf chondrocytes with shaping on one (saucer), two (cup), or neither (disk) surfaces. The saucer and cup constructs had shapes distinguishable quantitatively (radius of curvature of 5.5 ± 0.1 mm for saucer and 2.8 ± 0.1 mm for cup) and had no adverse effects on the glycosaminoglycan and collagen contents and their distribution in the constructs as assessed by biochemical assays and histology, respectively. Biomimetic stratification of chondrocyte subpopulations in saucer- and cup-shaped constructs was confirmed and quantified using fluorescence microscopy and image analysis. This shaping method, combined with biomimetic stratification, has the potential to create anatomically contoured large cartilaginous constructs.

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Copyright information

© The Association of Bone and Joint Surgeons 2008

Authors and Affiliations

  • EunHee Han
    • 1
  • Won C. Bae
    • 2
  • Nancy D. Hsieh-Bonassera
    • 3
  • Van W. Wong
    • 1
  • Barbara L. Schumacher
    • 1
  • Simon Görtz
    • 4
  • Koichi Masuda
    • 5
  • William D. Bugbee
    • 4
  • Robert L. Sah
    • 1
    • 6
  1. 1.Department of BioengineeringUniversity of California-San DiegoLa JollaUSA
  2. 2.Department of RadiologyUniversity of California-San DiegoLa JollaUSA
  3. 3.Department of Mechanical and Aerospace EngineeringUniversity of California-San DiegoLa JollaUSA
  4. 4.Department of Orthopaedic SurgeryUniversity of California-San DiegoLa JollaUSA
  5. 5.Department of Orthopedic Surgery and BiochemistryRush Medical College at Rush University Medical CenterChicagoUSA
  6. 6.Whitaker Institute of Biomedical EngineeringUniversity of California-San DiegoLa JollaUSA

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