Food and Bioprocess Technology

, Volume 10, Issue 5, pp 901–913 | Cite as

Effects of Vacuum Impregnation with Calcium Lactate and Pectin Methylesterase on Quality Attributes and Chelate-Soluble Pectin Morphology of Fresh-Cut Papayas

  • Hongshun Yang
  • Qiongying Wu
  • Li Ying Ng
  • Shifei Wang
Original Paper

Abstract

Vacuum impregnation was used to improve the quality attributes of fresh-cut papayas. Vacuum pressure of 5 kPa was applied for 5 min, then calcium lactate (1%, w/w) and pectin methylesterase (PME) (15 U/ml), alone and in combinations (calcium lactate plus PME), were vacuum impregnated into fresh-cut papaya cubes. Papaya cubes were stored at 4 °C, and the quality of fresh-cut papaya was studied at intervals for 8 days. The hardness and chewiness levels of fresh-cut papayas that were treated with calcium lactate and PME were 8.02 and 7.83 times of untreated fresh-cut papayas at day 8, respectively. After vacuum impregnation, colour of fresh-cut papayas changed significantly (P < 0.05) and an overall weight loss was observed as well. Chelate-soluble pectin (CSP) was extracted and its content correlated well with texture properties of fresh-cut papayas. Qualitative and quantitative analyses of CSP were conducted using atomic force microscopy. The proportion of chain widths greater than 45 nm had increased 35.0% in fresh-cut papayas vacuum impregnated with calcium lactate and PME at the end of storage. The results indicate that a combination of calcium ions and PME was able to maximally preserve the quality attributes of fresh-cut papayas and extend the shelf life.

Keywords

Vacuum impregnation Fresh-cut papaya Calcium lactate Pectin methylesterase Chelate-soluble pectin Atomic force microscopy 

References

  1. Adams, E. L., Kroon, P. A., Williamson, G., & Morris, V. J. (2003). Characterisation of heterogeneous arabinoxylans by direct imaging of individual molecules by atomic force microscopy. Carbohydrate Research, 338(8), 771–780.CrossRefGoogle Scholar
  2. Anjongsinsiri, P. B., Kenney, J., & Wicker, L. (2004). Detection of vacuum infusion of pectinmethylesterase in strawberry by activity staining. Journal of Food Science, 69(3), FCT179–FCT183.Google Scholar
  3. Boon, C. S., McClements, D. J., Weiss, J., & Decker, E. A. (2010). Factors influencing the chemical stability of carotenoids in foods. Critical Reviews in Food Science and Nutrition, 50(6), 515–532.CrossRefGoogle Scholar
  4. Brummell, D. A. (2006). Cell wall disassembly in ripening fruit. Functional Plant Biology, 33(2), 103–119.CrossRefGoogle Scholar
  5. Canizares, D., & Mauro, M. A. (2015). Enhancement of quality and stability of dried papaya by pectin-based coatings as air-drying pretreatment. Food and Bioprocess Technology, 8(6), 1187–1197.CrossRefGoogle Scholar
  6. Chávez-Sánchez, I., Carrillo-López, A., Vega-García, M., & Yahia, E. M. (2013). The effect of antifungal hot-water treatments on papaya postharvest quality and activity of pectinmethylesterase and polygalacturonase. Journal of Food Science and Technology, 50(1), 101–107.CrossRefGoogle Scholar
  7. Chen, F., Zhang, L., An, H., Yang, H., Sun, X., Liu, H., Yao, Y., & Li, L. (2009). The nanostructure of hemicellulose of crisp and soft Chinese cherry (Prunus pseudocerasus L.) cultivars at different stages of ripeness. LWT - Food Science and Technology, 42(1), 125–130.CrossRefGoogle Scholar
  8. Chen, Y., Chen, F., Lai, S., Yang, H., Liu, H., Liu, K., Bu, G., & Deng, Y. (2013). In vitro study of the interaction between pectinase and chelate-soluble pectin in postharvest apricot fruits. European Food Research and Technology, 237(6), 987–993.CrossRefGoogle Scholar
  9. Chiralt, A., & Talens, P. (2005). Physical and chemical changes induced by osmotic dehydration in plant tissues. Journal of Food Engineering, 67(1), 167–177.CrossRefGoogle Scholar
  10. Chong, J. X., Lai, S., & Yang, H. (2015). Effects of salt and sugar addition on the physicochemical properties and nanostructure of fish gelatin. Food Hydrocolloids, 45, 72–82.CrossRefGoogle Scholar
  11. Culver, C. A., Bjurlin, M. A., & Fulcher, R. G. (2000). Visualizing enzyme infusion into apple tissue. Journal of Agricultural and Food Chemistry, 48(12), 5933–5935.CrossRefGoogle Scholar
  12. Derossi, A., Pilli, T. D., & Severini, C. (2013). Application of vacuum impregnation techniques to improve the pH reduction of vegetables: study on carrots and eggplants. Food and Bioprocess Technology, 6(11), 3217–3226.CrossRefGoogle Scholar
  13. Feng, X., Lai, S., Li, M., Fu, C., Chen, F., & Yang, H. (2014). Application of atomic force microscopy in food-related macromolecules. In H. Yang (Ed.), Atomic force microscopy (AFM): principles, modes of operation and limitations. Hauppauge, NY: Nova Science Publishers, Inc..Google Scholar
  14. Fito, P., Chiralt, A., Barat, J. M., Andrés, A., Martínez-Monzó, J., & Martínez-Navarrete, N. (2001). Vacuum impregnation for development of new dehydrated products. Journal of Food Engineering, 49(4), 297–302.CrossRefGoogle Scholar
  15. Fraeye, I., Knockaert, G., Van Buggenhout, S., Duvetter, T., Hendrickx, M., & Van Loey, A. (2010). Enzyme infusion prior to thermal/high pressure processing of strawberries: mechanistic insight into firmness evolution. Innovative Food Science and Emerging Technologies, 11(1), 23–31.CrossRefGoogle Scholar
  16. Gayosso-García Sancho, L. E., Yahia, E. M., & González-Aguilar, G. A. (2011). Identification and quantification of phenols, carotenoids, and vitamin C from papaya (Carica papaya L. cv. Maradol) fruit determined by HPLC-DAD-MS/MS-ESI. Food Research International, 44(5), 1284–1291.CrossRefGoogle Scholar
  17. Guillemin, A., Guillon, F., Degraeve, P., Rondeau, C., Devaux, M.-F., Huber, F., Badel, E., Saurel, R., & Lahaye, M. (2008). Firming of fruit tissues by vacuum-infusion of pectin methylesterase: visualisation of enzyme action. Food Chemistry, 109(2), 368–378.CrossRefGoogle Scholar
  18. Hodges, D. M., Forney, C. F., & Wismer, W. (2000). Processing line effects on storage attributes of fresh-cut spinach leaves. Hortscience, 35(7), 1308–1311.Google Scholar
  19. Karakurt, Y., & Huber, D. J. (2003). Activities of several membrane and cell-wall hydrolases, ethylene biosynthetic enzymes, and cell wall polyuronide degradation during low-temperature storage of intact and fresh-cut papaya (Carica papaya) fruit. Postharvest Biology and Technology, 28(2), 219–229.CrossRefGoogle Scholar
  20. Lara, I., Garcia, P., & Vendrell, M. (2004). Modifications in cell wall composition after cold storage of calcium-treated strawberry (Fragaria × ananassa Duch.) fruit. Postharvest Biology and Technology, 34(3), 331–339.CrossRefGoogle Scholar
  21. Li, M., Chen, F., Yang, B., Lai, S., Yang, H., Liu, K., Bu, G., & Deng, Y. (2015). Preparation of organic tofu using organic compatible magnesium chloride incorporated with polysaccharide coagulants. Food Chemistry, 167, 168–174.CrossRefGoogle Scholar
  22. Liu, H., Chen, F., Yang, H., Yao, Y., Gong, X., Xin, Y., & Ding, C. (2009). Effect of calcium treatment on nanostructure of chelate-soluble pectin and physicochemical and textural properties of apricot fruits. Food Research International, 42(8), 1131–1140.CrossRefGoogle Scholar
  23. Liu, H., Chen, F., Lai, S., Tao, J., Yang, H., & Jiao, Z. (2017). Effects of calcium treatment and low temperature storage on cell wall polysaccharide nanostructures and quality of postharvest apricot (Prunus armeniaca). Food Chemistry, 225, 87–97.CrossRefGoogle Scholar
  24. Liu, Q., Tan, C. S. C., Yang, H., & Wang, S. (2016). Treatment with low-concentration acidic electrolysed water combined with mild heat to sanitise fresh organic broccoli (Brassica oleracea). LWT-Food Science and Technology. doi:10.1016/j.lwt.2016.11.012.Google Scholar
  25. Manrique, G. D., & Lajolo, F. M. (2004). Cell-wall polysaccharide modifications during postharvest ripening of papaya fruit (Carica papaya). Postharvest Biology and Technology, 33, 11–26.Google Scholar
  26. Mao, J., Zhang, L., Chen, F., Lai, S., Yang, B., & Yang, H. (2016). Effect of vacuum impregnation combined with calcium lactate on the firmness and polysaccharide morphology of Kyoho grapes (Vitis vinifera × V. labrusca). Food and Bioprocess Technology. doi:10.1007/s11947-016-1852-5.Google Scholar
  27. Martin-Diana, A. B., Rico, D., Frias, J. M., Barat, J. M., Henehan, G. T. M., & Barry-Ryan, C. (2007). Calcium for extending the shelf life of fresh whole and minimally processed fruits and vegetables: a review. Trends in Food Science & Technology, 18(4), 210–218.CrossRefGoogle Scholar
  28. Ornelas-Paz, J. D. J., Yahia, E. M., & Gardea, A. A. (2008). Changes in external and internal color during postharvest ripening of ‘manila’ and ‘Ataulfo’ mango fruit and relationship with carotenoid content determined by liquid chromatography–APcI+−time-of-flight mass spectrometry. Postharvest Biology and Technology, 50(2), 145–152.CrossRefGoogle Scholar
  29. Perez-Cabrera, L., Chafer, M., Chiralt, A., & Gonzalez-Martinez, C. (2011). Effectiveness of antibrowning agents applied by vacuum impregnation on minimally processed pear. LWT-Food Science and Technology, 44(10), 2273–2280.CrossRefGoogle Scholar
  30. Sancho, L. E. G.-G., Yahia, E. M., García-Solís, P., & González-Aguilar, G. A. (2014). Inhibition of proliferation of breast cancer cells MCF7 and MDA-MB-231 by lipophilic extracts of papaya (Carica papaya L. Var. Maradol) fruit. Food and Nutrition Sciences, 5(21), 2097–2103.CrossRefGoogle Scholar
  31. Shih, M. C., Yang, K. T., & Kuo, S. J. (2002). Quality and antioxidative activity of black soybean tofu as affected by bean cultivar. Journal of Food Science, 67(2), C480–C484.Google Scholar
  32. Sirijariyawat, A., Charoenrein, S., & Barrett, D. M. (2012). Texture improvement of fresh and frozen mangoes with pectin methylesterase and calcium infusion. Journal of the Science of Food and Agriculture, 92(13), 2581–2586.CrossRefGoogle Scholar
  33. Talens, P., Martínez-Navarrete, N., Fito, P., & Chiralt, A. (2002). Changes in optical and mechanical properties during osmodehydrofreezing of kiwi fruit. Innovative Food Science and Emerging Technologies, 3(2), 191–199.CrossRefGoogle Scholar
  34. Toivonen, P. M. A., & Brummell, D. A. (2008). Biochemical bases of appearance and texture changes in fresh-cut fruit and vegetables. Postharvest Biology and Technology, 48(1), 1–14.CrossRefGoogle Scholar
  35. Udomkun, P., Mahayothee, B., Nagle, M., & Müller, J. (2014). Effects of calcium chloride and calcium lactate applications with osmotic pretreatment on physicochemical aspects and consumer acceptances of dried papaya. International Journal of Food Science & Technology, 49(4), 1122–1131.CrossRefGoogle Scholar
  36. Wakabayashi, K. (2000). Changes in cell wall polysaccharides during fruit ripening. Journal of Plant Research, 113(3), 231–237.CrossRefGoogle Scholar
  37. Xin, Y., Chen, F., Yang, B., Yang, H., Zhang, P., & Deng, Y. (2010). Morphology, profile and role of chelate-soluble pectin on tomato properties during ripening. Food Chemistry, 121(2), 372–380.CrossRefGoogle Scholar
  38. Yang, H., Chen, F., An, H., & Lai, S. (2009). Comparative studies on nanostructures of three kinds of pectins in two peach cultivars using atomic force microscopy. Postharvest Biology and Technology, 51(3), 391–398.CrossRefGoogle Scholar
  39. Yang, H., Wang, Y., Jiang, M., Oh, J. H., Herring, J., & Zhou, P. (2007a). 2-step optimization of the extraction and subsequent physical properties of channel catfish (Ictalurus punctatus) skin gelatin. Journal of Food Science, 72(4), C188–C195.CrossRefGoogle Scholar
  40. Yang, H., Wang, Y., Lai, S., An, H., Li, Y., & Chen, F. (2007b). Application of atomic force microscopy as a nanotechnology tool in food science. Journal of Food Science, 72(4), R65–R75.CrossRefGoogle Scholar
  41. Yu, X., & Yang, H. (2017). Pyrethroid residue determination in organic and conventional vegetables using liquid-solid extraction coupled with magnetic solid phase extraction based on polystyrene-coated magnetic nanoparticles. Food Chemistry, 217, 303–310.CrossRefGoogle Scholar
  42. Zhang, J., & Yang, H. (2017). Effects of potential organic compatible sanitisers on organic and conventional fresh-cut lettuce (Lactuca sativa Var. Crispa L). Food Control, 72, 20–26.CrossRefGoogle Scholar
  43. Zhang, L., Chen, F., An, H., Yang, H., Sun, X., Guo, X., & Li, L. (2008). Physicochemical properties, firmness, and nanostructures of sodium carbonate-soluble pectin of 2 Chinese cherry cultivars at 2 ripening stages. Journal of Food Science, 73(6), N17–N22.CrossRefGoogle Scholar
  44. Zhang, L., Chen, F., Zhang, P., Lai, S., & Yang, H. (2017). Influence of rice bran wax coating on the physicochemical properties and pectin nanostructure of cherry tomatoes. Food and Bioprocess Technology, 10, 349–357.Google Scholar
  45. Zhao, L., Zhang, Y., & Yang, H. (2017). Efficacy of low concentration neutralised electrolysed water and ultrasound combination for inactivating Escherichia coli ATCC 25922, Pichia pastoris GS115 and Aureobasidium pullulans 2012 on stainless steel coupons. Food Control, 73, 889–899.CrossRefGoogle Scholar
  46. Zhao, Y., & Xie, J. (2004). Practical applications of vacuum impregnation in fruit and vegetable processing. Trends in Food Science & Technology, 15(9), 434–451.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Food Science and Technology Programme, c/o Department of ChemistryNational University of SingaporeSingaporeRepublic of Singapore
  2. 2.National University of Singapore (Suzhou) Research InstituteSuzhouPeople’s Republic of China
  3. 3.School of BiotechnologyJiangsu University of Science and TechnologyZhenjiangPeople’s Republic of China
  4. 4.Changzhou Qihui Management and Consulting Co., Ltd.ChangzhouPeople’s Republic of China

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