Effects of Fish Gelatin and Tea Polyphenol Coating on the Spoilage and Degradation of Myofibril in Fish Fillet During Cold Storage
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Fish fillet is easily spoiled during storage. Antimicrobial edible coating of gelatin extracted from fish skins and bones and tea polyphenol (TP) was developed to inhibit the spoilage of fish fillet during cold storage. For coating containing 0.4 % TP and 1.2 % gelatin, the pH only slightly increased from 6.17 at day 0 to 6.32 at day 17 of cold storage, while the pH of control coating increased to 6.87 at day 17. Atomic force spectrometry was used to analyse the nanostructure of myofibril, which is the major component of fish muscle. The results showed that the length of myofibril from 0.4 % TP and 1.2 % gelatin group was greater than 15 μm, while the diameter and height were 3.38 and 0.59 μm, respectively, which exhibited the most intact nanostructure after 17 days of cold storage. Meanwhile, matrix-assisted laser desorption–ionisation–time-of-flight mass spectrometry result showed that TP delayed the degradation of myosin light chain 3 and troponin T in myofibril. Gas chromatography–mass spectrometry of volatile organic compounds (VOCs) also showed that 0.4 % TP and 1.2 % gelatin coating group had minimal production of spoilage markers, such as 1-octen-3-ol, 2-methyl propanoic acid and dimethyl sulfide. The microbial analysis showed that the aerobic mesophilic/psychrotrophic count, yeasts and moulds of 0.4 % TP and 1.2 % gelatin group were significantly lower than the control group. Therefore, 0.4 % TP and 1.2 % gelatin coating showed the best antimicrobial effect and can be used to maintain the nanostructure of fish fillet and prevent the spoilage during cold storage.
KeywordsGelatin coating Tea polyphenol Matrix-assisted laser desorption–ionisation–time-of-flight mass spectrometry (MALDI-TOF-MS) Atomic force spectrometry (AFM) Headspace solid-phase microextraction–gas chromatography–mass spectrometry (HS/SPME/GC/MS)
We acknowledge the financial support by Singapore Ministry of Education Academic Research Fund Tier 1 (R-143-000-583-112) and the start-up grant (R-143-000-561-133) by the National University of Singapore. Projects 31371851, 31071617, 31471605 and 31200801 supported by NSFC, Natural Science Foundation of Jiangsu Province (BK20141220) and Applied Basic Research Project (Agricultural) Suzhou Science and Technology Planning Programme (SYN201522) also contributed to this research.
- Ayala, M.D., Santaella, M., Martínez, C., Periago, M.J., Blanco, A., Vázquez, J.M., & Albors, O.L. (2011). Muscle tissue structure and flesh texture in gilthead sea bream, Sparus aurata L., fillets preserved by refrigeration and by vacuum packaging. LWT-Food Science and Technology, 44, 1098–1106.Google Scholar
- Capitani, M.I., Matus-Basto, A., Ruiz-Ruiz, J.C., Santiago-García, J.L., Betancur-Ancona, D.A., Nolasco, S.M., Tomás, M.C., & Segura-Campos, M.R. (2016). Characterization of biodegradable films based on Salvia hispanica L. protein and mucilage. Food and Bioprocess Technology, 1–11.Google Scholar
- Chen, T., & Levin, R. (1974). Taxonomic significance of phenethyl alcohol production by Achromobacter isolates from fishery sources. Applied Microbiology, 28, 681–687.Google Scholar
- Chen, B.J., Zhou, Y.J., Wei, X.Y., Xie, H.J., Hider, R.C., & Zhou, T. (2016). Edible antimicrobial coating incorporating a polymeric iron chelator and its application in the preservation of surimi product. Food and Bioprocess Technology, 1–9.Google Scholar
- Feng, X., Lai, S., & Yang, H. (2014). Sustainable seafood processing: utilisation of fish gelatin. Austin Journal of Nutrition and Food Sciences, 2, 1006.Google Scholar
- Iglesias, J., Medina, I., Bianchi, F., Careri, M., Mangia, A., & Musci, M. (2009). Study of the volatile compounds useful for the characterisation of fresh and frozen-thawed cultured gilthead sea bream fish by solid-phase microextraction gas chromatography–mass spectrometry. Food Chemistry, 115, 1473–1478.CrossRefGoogle Scholar
- Iwasaki, T., Hasegawa, Y., Yamamoto, K., & Nakamura, K. (2009). The relationship between the changes in local stiffness of chicken myofibril and the tenderness of muscle during postmortem aging. In Gels: structures, properties, and functions, 205–210.Google Scholar
- Leisner, J. J., & Gram, L. (2000). Spoilage of fish. In Encyclopedia of food microbiology. Academic Press, Incorporated.Google Scholar
- Qian, Y. F., Xie, J., Yang, S. P., Huang, S., Wu, W. H., & Li, L. (2015). Inhibitory effect of a quercetin-based soaking formulation and modified atmospheric packaging (MAP) on muscle degradation of Pacific white shrimp (Litopenaeus vannamei). LWT--Food Science and Technology, 63, 1339–1346.CrossRefGoogle Scholar
- Shiroodi, S.G., Nesaei, S., Ovissipour, M., Al-Qadiri, H.M., Rasco, B., & Sablani, S. (2016). Biodegradable polymeric films incorporated with nisin: characterization and efficiency against Listeria monocytogenes. Food and Bioprocess Technology, 1–12.Google Scholar
- Wu, C., Li, Y., Wang, L., Hu, Y., Chen, J., Liu, D., & Ye, X. (2016). Efficacy of chitosan-gallic acid coating on shelf life extension of refrigerated pacific mackerel fillets. Food and Bioprocess Technology, 1–11.Google Scholar