Combination of κ-Carrageenan and Soy Protein Isolate Effects on Functional Properties of Chopped Low-Fat Pork Batters During Heat-Induced Gelation
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The current study was conducted to investigate combination impacts of κ-carrageenan (CAR) and soy protein isolate (SPI) on the functional properties of chopped low-fat pork batters during heat-induced gelation. Texture profile analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Raman spectroscopy, and scanning electron microscopy (SEM) were used to determine the properties of pork batters. The addition of individual CAR and SPI or CAR/SPI mixture improved textural properties except for cohesiveness compared with the control (P < 0.05). The cooking loss with supplemented ingredients was significantly lowered, while the thermal emulsion stability was improved compared to that of the control (P < 0.05). Compared with the control, the CAR/SPI mixture formed a more continuous structure evidenced by more uniform fat particles and a more compact protein matrix. Raman spectroscopy showed that pork batters with ingredients resulted in a lower content of α-helice and a higher content of β-sheet, β-turn, and random coil compared with the control. However, no interactions between meat protein and CAR/SPI could be identified by SDS-PAGE profiles. The results indicate that the combination of CAR/SPI can be effectively used to improve functional properties of low-fat pork batters effectively.
KeywordsMeat protein Carrageenan Soy protein isolate Functional properties Chopped pork batters
Soy protein isolates
κ-Carrageenan and soy protein isolate mixture
Texture profile analysis
Total fluid release
This research was funded by the Twelfth Five Issues of Rural Areas of People’s Republic of China (2012BAD28B03).
- Bernal, V. M., Smajda, C. H., Smith, J. L., & Stanley, D. W. (1987). Interactions in protein/polysaccharide/calcium gels. Journal of Food Science, 52(1121–1125), 1136.Google Scholar
- Liu, K. (2000). Expanding soybean food utilization. Food Technology, 54, 46–58.Google Scholar
- Perisic, N., Afsetha, N. K., Ofstada, R., Hassani, S., Kohler, A. (2013). Characterising protein, salt and water interactions with combined vibrational spectroscopic techniques. Food Chemistry, 138, 679–686Google Scholar
- Sebranek, J. G. (2009). Functional properties of muscle proteins: implications for processed meat product characteristics. In K. Johnson (Ed.), Proceedings of 62nd Reciprocal Meat Conference (pp. 39–45). Arkansas: Rogers.Google Scholar
- Serdaroğlu, M., & Sapancı-Özsümer, M. (2003). Effects of soy protein, whey powder and wheat gluten on quality characteristics of cooked beef sausages formulated with 5, 10 and 20% fat. Electronic Journal of Polish Agricultural Universities Series: Food Science and Technology, 6, 1–9.Google Scholar
- Şükrü, K., & Osman, K. (2012). The effects of cereal and legume flours on the quality characteristics of beef patties. Kafkas Universitesi Veteriner Fakultesi Dergisi, 18(5), 725–730.Google Scholar
- Tu, A. (1982). Raman spectroscopy in biology. New York: John Wiley.Google Scholar