Food and Bioprocess Technology

, Volume 8, Issue 6, pp 1169–1186 | Cite as

Review on the Current State of Diacylglycerol Production Using Enzymatic Approach

  • Eng-Tong Phuah
  • Teck-Kim Tang
  • Yee-Ying Lee
  • Thomas Shean-Yaw Choong
  • Chin-Ping Tan
  • Oi-Ming LaiEmail author
Original Paper


Enzymatic production of diacylglycerol (DAG)-enriched oil has been investigated extensively due to its health benefits with total annual sales of approximately USD 200 million in Japan since its introduction in the late 1990s till 2009. Enzymatic catalysis had been proven to exhibit improved results with respect to yield, purity, reaction time, and stability in comparison with chemical catalysis. The cost of the enzymes, however, is the main hurdle to the widespread use of enzyme for commercial DAG production. This paper attempts to review and summarize various lipase-mediated technological methods for DAG production. Critical aspects such as process considerations on DAG synthesis, mass transfer limitations as well as kinetic mechanism models developed for each enzymatic approach in DAG synthesis are also presented and discussed. In addition, possible reactor configurations were evaluated, if lipase-assisted DAG production is to be technically and economically feasible at an industrial scale.


Diacylglycerol Enzyme lipase Obesity Reactor Kinetic study 



The authors thank Sime Darby Research Sdn. Bhd. and the Ministry of Science, Technology and Innovation Malaysia (MOSTI) for their financial supports.


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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Eng-Tong Phuah
    • 1
  • Teck-Kim Tang
    • 1
  • Yee-Ying Lee
    • 1
  • Thomas Shean-Yaw Choong
    • 2
    • 3
  • Chin-Ping Tan
    • 4
  • Oi-Ming Lai
    • 1
    • 5
    Email author
  1. 1.Institute of BioscienceUniversiti Putra MalaysiaSerdangMalaysia
  2. 2.Department of Chemical Engineering, Faculty of EngineeringUniversiti Putra MalaysiaSerdangMalaysia
  3. 3.INTROP, Universiti Putra MalaysiaSerdangMalaysia
  4. 4.Department of Food Technology, Faculty of Food Science and TechnologyUniversiti Putra MalaysiaSerdangMalaysia
  5. 5.Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular SciencesUniversiti Putra MalaysiaSerdangMalaysia

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