Food and Bioprocess Technology

, Volume 8, Issue 1, pp 54–62 | Cite as

Efficacy of Lactoperoxidase System-Whey Protein Coating on Shelf-life Extension of Rainbow Trout Fillets During Cold Storage(4 °C)

  • Sajad Shokri
  • Ali Ehsani
  • Mohammad Sedigh Jasour
Original Paper

Abstract

The growth and metabolism of gram-negative microorganisms are widely recognized as important reasons for the spoilage of fish and fish product. Based on antimicrobial activity of lactoperoxidase system (LPOS) against gram-negative bacteria, the present study investigated the effect of incorporation of LPOS at concentrations 0 (control), 1.25, 2.5, 5 and 7.5 % (v/v) into whey protein solution as novel antimicrobial coating strategy for rainbow trout (Oncorhynchus mykiss) preservation under refrigeration temperature (4 ± 1 °C) over a 16-day period. During storage of trout fillets, control and 1.25 % LPOS-treated samples reached undesirable levels of rancidity (p < 0.05) at 12th and 16th days, respectively, whereas the higher concentrations showed to be more efficiently stable (p < 0.05) in sensory, chemical (total volatile basic nitrogen (TVB-N) and pH) and microbiological analyses (mesophilic bacteria, psychrotrophic bacteria, Pseudomonas spp. and specific spoilage bacteria). Lipid oxidation in the fillets was not affected significantly by LPOS treatment. The LPOS-whey protein coating also reduced total specific spoilage organisms (Shewanella putrefaciens and Pseudomonas fluorescens) of the fillets more than 1.5 log by the end of storage. The shelf life of samples was extended approximately from 12 days to at least 16 days in the presence of the LPOS. These results indicated the significant antimicrobial activity of the LPOS-whey protein coating solution for refrigerated fish fillets.

Keywords

Rainbow trout Antimicrobial coating Lactoperoxidase Whey protein Shelf life 

Abbreviations

LPOS

Lactoperoxidase system

SSO

Specific spoilage organisms

LPO

Lactoperoxidase

SCN

Thiocyanate

H2O2

Hydrogen peroxide

OSCN

Hypothiocyanite

HOSCN

Hypothiocyanous acid

SH

Sulphydryl

GO

Glucose oxidase

Glu

d-α-Glucose

KSCN

Potassium thiocyanate

WPS

Whey protein solution

TVB-N

Total volatile basic nitrogen

TBA

Thiobarbituric acid

MA

Malonaldehyde

Notes

Acknowledgments

This work was financially supported by the Urmia University (Urmia, Iran). The authors thank Ms. Seyedeh-Samaneh Naghibi for her excellent technical assistance. The facilities provided by the Artemia and Aquatics’ Research Institute is also gratefully acknowledged.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Sajad Shokri
    • 1
  • Ali Ehsani
    • 1
  • Mohammad Sedigh Jasour
    • 2
  1. 1.Department of Food Hygiene, Faculty of Veterinary MedicineUrmia UniversityUrmiaIran
  2. 2.Artemia and Aquatics’ Research InstituteUrmia UniversityUrmiaIran

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