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Food and Bioprocess Technology

, Volume 7, Issue 11, pp 3328–3335 | Cite as

Stimulation of Saccharomyces cerevisiae Cultures by Pulsed Electric Fields

  • Jessy R. Mattar
  • Mohammad F. Turk
  • Maurice Nonus
  • Nikolai I. Lebovka
  • Henri El Zakhem
  • Eugene Vorobiev
Original Paper

Abstract

The effects of stimulation of Saccharomyces cerevisiae cells in an aqueous suspension by pulsed electric field (PEF) with electric field strength E = 20–2,000 V cm−1 and effective PEF treatment time t PEF = 10−5–1 s were investigated. At relatively high electric field strengths (E > 1,000 V cm−1) and moderate times of PEF treatment (t PEF > 100 μs), the extraction of ionic components from yeast was observed, which can be related to electroporation of cell membranes. Petri dishes counting revealed dependency of the colony sizes on the time of preliminary fermentation t f and power consumption W. The “logarithmic” and “saturated” types of electrostimulation were distinguished. At “logarithmic” electrostimulation (10−7 J mL−1 < W < 10−1 J mL−1), the viability of yeast cells increased with the increase of power consumption and was higher for longer fermentation (t f  = 24 h). However, at “saturated” electrostimulation (10−1 J mL−1 < W < 101 J mL−1), the viability of yeast cells was noticeably higher for t f  = 1 h than for t f  = 24 h. The impact of preliminary fermentation time and PEF protocol on biological activity of cells and consumption of nutrients was also discussed.

Keywords

Pulsed electric field Electroporation Stimulation Brewer’s yeast S. cerevisiae 

Notes

Acknowledgments

The authors appreciate the financial support from the financial support from the CNRS-Lebanon and the support from the COST Action TD1104 (EP4Bio2Med-European Network for Development of Electroporation-based Technologies and treatments). The authors also thank Dr. N.S. Pivovarova for her help with the preparation of the manuscript.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Jessy R. Mattar
    • 1
    • 2
  • Mohammad F. Turk
    • 3
  • Maurice Nonus
    • 1
  • Nikolai I. Lebovka
    • 1
    • 4
  • Henri El Zakhem
    • 2
  • Eugene Vorobiev
    • 1
  1. 1.Équipe TAI EA TIMR 4297, Université de Technologie de CompiègneCentre de Recherche de RoyallieuCompiègne cedexFrance
  2. 2.Chemical Engineering DepartmentUniversity of BalamandAmiounLebanon
  3. 3.Équipe TAI EA TIMR 4297École Supérieure de Chimie Organique et MinéraleCompiègne cedexFrance
  4. 4.Department of Physical Chemistry of Disperse MineralsInstitute of Biocolloidal Chemistry named after F.D. Ovcharenko, NAS of UkraineVernadskogoUkraine

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