Food and Bioprocess Technology

, Volume 7, Issue 3, pp 821–828 | Cite as

Cronobactersakazakii Inactivation by Microwave Processing

  • Maria C. Pina-Pérez
  • Maria Benlloch-Tinoco
  • Dolores Rodrigo
  • Antonio Martinez
Original Paper


Nowadays, current practices at home and childcare settings involved MW heating after powder infant formula milk reconstitution. Microwave (MW) effectiveness for Cronobacter sakazakii inactivation in reconstituted powder infant formula milk was investigated. Power levels in the range 400–900 W were tested during exposure times ranging from 0 to 120 s. Power levels of 800 and 900 W reduced the C. sakazakii initial population to undetectable levels (≥8 log10 cycles), reaching maximum temperatures of 78.8 ± 2.3 °C and 88.1 ± 1.5 °C, respectively. A post-treatment storage study (5 °C, 24 h) was completed to determine the recovery or death of C. sakazakii damaged and survival cells. It was observed that lowest MW intensity treatments (power level and treatment time) provide the highest percentages of injured cells. Moreover, these high percentages of damaged cells progressively die during refrigerated storage (up to 24 h). Microbial reduction levels higher or equal to 5 log10 cycles were reached due to the sublethal damaged cell death.


Microwave processing Cronobacter sakazakii Refrigeration Sublethal damage Reconstituted powder infant formula milk 



The authors are grateful to the Ministry of Economy and Competitiveness for providing financial support by means of CYCIT project AGL2010-22206-C02, and to Generalitat Valenciana I+D+I emergent research groups financial support in project GV/2010/064.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Maria C. Pina-Pérez
    • 1
  • Maria Benlloch-Tinoco
    • 2
  • Dolores Rodrigo
    • 1
  • Antonio Martinez
    • 1
  1. 1.Departamento Conservación y CalidadInstituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC)PaternaSpain
  2. 2.Departamento Tecnología de Alimentos, Grupo Investigación e Innovación en AlimentosUniversidad Politécnica de ValenciaCamino de Vera, s/nSpain

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