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Food and Bioprocess Technology

, Volume 6, Issue 7, pp 1729–1740 | Cite as

Mozzarella-Type Curd Made from Buffalo, Cows’ and Ultrafiltered Cows’ Milk. 1. Rheology and Microstructure

  • Imtiaz HussainEmail author
  • Alan E. Bell
  • Alistair S. Grandison
Original Paper

Abstract

Rennet-induced curd was made from both natural buffalo and cows’ milk, and ultrafiltered cows’ milk (cows’ milk was concentrated such that it had a chemical composition approximately equivalent to that of the buffalo milk). These milk samples were compared on the basis of their rheology, physicochemical characteristics and curd microstructure. The ionic and soluble calcium contents were found to be similar in all milk samples studied. The total and casein bound calcium were higher in concentrated cows’ milk than in standard cows’ milk. Both cows’ milk types were found to have lower total and casein bound calcium than the buffalo milk. This is probably due to concentration of the colloidal part of milk (casein), during the ultrafiltration (UF) process. The rennet coagulation time was similar in UF cows’ and buffalo milk while both were shorter when compared with that of the cows’ milk. The dynamic moduli (G′, G″) values were higher in both the buffalo and UF cows’ milk than in the cows’ milk after 90 min coagulation. The loss tangent, however, was found to be similar in both the UF cows’ and buffalo milk curds and was lower than that observed for the cows’ milk (0.42, 0.42 and 0.48, respectively). The frequency profile of each type of curd was recorded 90 min after the enzyme addition (0.1–10 Hz); all samples were found to be “weak” viscoelastic, frequency dependent gels. The yield stress was also measured 95 min after the enzyme addition, and a higher value was observed in buffalo milk curd when compared with other curd samples made from both the natural cows’ milk and the UF cows’ milk. The cryo-scanning electron and confocal laser scanning micrographs showed that curd structure appeared to be more “dense” and less porous in buffalo milk than cows’ milk even after concentration to equivalent levels of protein/total solids to those found in the buffalo milk.

Keywords

Rheology Microstructure Mozzarella curd Buffalo Cows’ Ultrafiltration 

Notes

Acknowledgments

The authors thank Dr. Chris J. Stain, Center for Advanced Microscopy, for technical assistance during the image analysis.

References

  1. Abd El-Gawad, M. A. M., & Ahmed, N. S. (2011). Cheese yield as affected by some parameters review. Acta Scientiarum Polonorum, Technologia Alimentaria, 10(2), 131–153.Google Scholar
  2. Ahmad, S., Gaucher, I., Rousseau, F., Beaucher, E., Piot, M., Grongnet, J. F., & Gaucheron, F. (2008). Effects of acidification on physico-chemical characteristics of buffalo milk: A comparison with cow’s milk. Food Chemistry, 106(1), 11–17.CrossRefGoogle Scholar
  3. Culioli, S., & Sherman, P. (1978). Rheological aspects of the renneting of milk concentrated by ultrafiltration. Journal of Texture Studies, 9(3), 257–281.CrossRefGoogle Scholar
  4. Dalgleish, D. G. (1981). Effect of milk concentration on the nature of curd formed during renneting—a theoretical discussion. The Journal of Dairy Research, 48(1), 65–69.CrossRefGoogle Scholar
  5. Davies, D. T., & White, J. C. D. (1960). The use of ultrafiltration and dialysis in isolating the aqueous phase of milk and in determining the partition of milk constituents between the aqueous and disperse phases. The Journal of Dairy Research, 27(2), 171–190.CrossRefGoogle Scholar
  6. Dimassi, O., Neidhard, S., Carle, R., Mertz, L., Migliore, G., Mané-Bielfeldt, A., & Valle Zárate, A. (2005). Cheese production potential of milk of Dahlem Cashmere goats from a rheological point of view. Small Ruminant Research, 57(1), 31–36.CrossRefGoogle Scholar
  7. Everett, D. W., & Auty, M. A. E. (2008). Cheese structure and current methods of analysis. International Dairy Journal, 18(7), 759–773.CrossRefGoogle Scholar
  8. Ferry, J. D. (1980). Viscoelastic properties of polymers (3rd ed.). Canada: John Wiley & Sons Inc.Google Scholar
  9. Green, M. L. (1987). Effects of manipulation of milk composition and curd forming conditions on the formation, structure and properties of milk curd. The Journal of Dairy Research, 54(3), 303–313.CrossRefGoogle Scholar
  10. Green, M. L., Langley, K. R., Marshal, R. J., Brooker, B. E., Willis, A., & Vincent, J. F. V. (1986). Mechanical properties of cheese, cheese analogues and protein gels in relation to composition and microstructure. Food Microstructure, 5(1), 169–180.Google Scholar
  11. Guinee, T. P., Pudja, P. D., & Mulholland, E. O. (1994). Effects of milk protein standardization, by ultrafiltration, on the manufacture, composition and maturation of cheddar cheese. The Journal of Dairy Research, 61(1), 117–131.CrossRefGoogle Scholar
  12. Guinee, T. P., O’Kennedy, B. T., & Kelly, P. M. (2006). Effect of milk protein standardization using different methods on the composition and yields of cheddar cheese. Journal of Dairy Science, 89(2), 468–482.CrossRefGoogle Scholar
  13. Hassan, A. N., & Award, S. (2005). Application of exopolysaccharide-producing cultures in reduced-fat cheddar cheese: Cryo-scanning electron microscopy observations. Journal of Dairy Science, 88(12), 4214–4220.CrossRefGoogle Scholar
  14. Hussain, I., Bell, A. E., & Grandison, A. S. (2011). Comparison of the rheology of Mozzarella-type curd made from buffalo and cows’ milk. Food Chemistry, 128(2), 500–504.CrossRefGoogle Scholar
  15. Impoco, G., Carrato, S., Caccamo, M., Tuminello, L., Licitra G. (2006). Image analysis methods for industrial application: quantitative analysis of cheese microstructure using SEM imagery. ADSA–ASAS joint Annual Meeting. Minneapolis, Minnesota, USA.Google Scholar
  16. Kuchroo, C. N., & Malik, R. C. (1976). Voluminosity and hydration of buffalo milk casein micelles. Milchwissenschaft, 31(1), 28–31.Google Scholar
  17. Kulmyrzaev, A. A., & Dufour, E. (2010). Relations between spectral and physicochemical properties of cheese, milk, and whey examined using multidimensional analysis. Food and Bioprocess Technology, 3(2), 247–256.CrossRefGoogle Scholar
  18. Lewis, M. J. (2011). The measurement and significance of ionic calcium in milk—A review. International Journal of Dairy Technology, 64(1), 1–13.CrossRefGoogle Scholar
  19. Lin, M.-J., Lewis, M. J., & Grandison, A. S. (2006). Measurement of ionic calcium in milk. International Journal of Dairy Technology, 59(3), 192–199.CrossRefGoogle Scholar
  20. Lucey, J. A. (2002). Formation and physical properties of milk protein gels. Journal of Dairy Science, 85(2), 281–294.CrossRefGoogle Scholar
  21. Menard, O., Ahmad, S., Rousseau, F., Briard-bion, V., Gaucheron, F., & Lopez, C. (2010). Buffalo vs. cow milk fat globules: Size distribution, zeta-potential, compositions in total fatty acids and in polar lipids from the milk fat globule membrane. Food Chemistry, 120(2), 544–551.CrossRefGoogle Scholar
  22. Mishra, R., Govindasamy-Lucey, S., & Lucey, J. A. (2005). Rheological properties of rennet induced gels during the coagulation and cutting process: Impact of processing conditions. Journal of Texture Studies, 36(2), 190–212.CrossRefGoogle Scholar
  23. Mistry, V. V., & Maubois, J. L. (1993). Application of membrane technology to cheese production. In P. F. Fox (Ed.), Cheese: Chemistry, physics and microbiology (General aspects 2nd ed., Vol. 1, pp. 493–522). London, UK: Chapman & Hall.CrossRefGoogle Scholar
  24. Ong, L., Dagastine, R. R., Kentish, S. E., & Gras, S. L. (2010). The effect of milk processing on the microstructure of the milk fat globule and rennet induced gel observed using confocal laser scanning microscopy. Journal of Food Science, 75(3), E135–E145.CrossRefGoogle Scholar
  25. Ong, L., Dagastine, R. R., Kentish, S. E., & Gras, S. L. (2011). Microstructure of milk gel and cheese curd observed using cryo scanning electron microscopy and confocal microscopy. LWT-Food Science and Technology, 44(5), 1291–1302.CrossRefGoogle Scholar
  26. Pignon, F., Belina, G., NarayananT, P. X., Magnin, A., & Gesan-Guiziou, G. (2004). Structure and rheological behavior of casein micelles suspensions during ultrafiltration process. Journal of Chemical Physics, 121(16), 8138–8146.CrossRefGoogle Scholar
  27. Raju, P. N., & Pal, D. (2009). The physico-chemical, sensory, and textural properties of Misti Dahi prepared from reduced fat buffalo milk. Food and Bioprocess Technology, 2(1), 101–108.CrossRefGoogle Scholar
  28. Sabarwal, P. K., & Ganguli, N. C. (1977). Studies on micellar kappa–casein of cow and buffalo milk in relation to sialic acid and rennet action. Milchwissenschaft, 32(4), 202–206.Google Scholar
  29. Sandra, S., Cooper, C., Alexander, M., & Corredig, M. (2011). Coagulation properties of ultrafiltered milk retentates measured using rheology and diffusing wave spectroscopy. Food Research International, 44(4), 951–956.CrossRefGoogle Scholar
  30. Sharma, S. K., Mittal, G. S., & Hill, A. R. (1994). Effects of milk concentration, pH and temperature on κ-casein hydrolysis at aggregation, coagulation and cutting times of ultrafiltrated milk. Milchwissenschaft, 49(2), 450–453.Google Scholar
  31. Sindhu, J.S., & Arora, S. (2011). Buffalo milk. Encyclopedia of dairy sciences (2nd ed.), pp. 503–511.Google Scholar
  32. Thomann, S. (2007). The impact of milk properties and process conditions on consistency of rennet-coagulated curd and syneresis of rennet curd grains. PhD Thesis. Institute of Food Science and Biotechnology, University of Hohenheim, Stuttgart, Germany.Google Scholar
  33. Tsioulpas, A., Lewis, M. J., & Grandison, A. S. (2007). Effect of minerals on casein micelle stability of cows’ milk. The Journal of Dairy Research, 74(2), 167–173.CrossRefGoogle Scholar
  34. Udabage, P., McKinnon, I. R., & Augustin, M. A. (2001). Effects of mineral salts and calcium chelating agents on the gelation of renneted skim milk. Journal of Dairy Science, 84(7), 1569–1575.CrossRefGoogle Scholar
  35. Van Vliet, T. (1999). Factors determining small-deformation behaviour of gels. In E. Dickinson & J. M. Rodriguez Patino (Eds.), Food emulsions and foams; interfaces, interactions and stability (pp. 307–317). Cambridge, UK: Royal Society of Chemistry.Google Scholar
  36. Walstra, P. (1993). The syneresis of curd. In P. F. Fox (Ed.), Cheese: Chemistry, physics and microbiology (General aspects, Vol. 1, pp. 141–191). London, UK: Chapman and Hall.CrossRefGoogle Scholar
  37. Zoon, P., van Vliet, T., & Walstra, P. (1988). Rheological properties of rennet-induced skim milk gels. 2. The effect of temperature. Netherlands Milk and Dairy Journal, 42(2), 249–269.Google Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Imtiaz Hussain
    • 1
    Email author
  • Alan E. Bell
    • 1
  • Alistair S. Grandison
    • 1
  1. 1.Department of Food and Nutritional SciencesUniversity of ReadingReadingUK

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