Food and Bioprocess Technology

, Volume 6, Issue 10, pp 2931–2937 | Cite as

Application of Visible and Near Infrared Hyperspectral Imaging to Differentiate Between Fresh and Frozen–Thawed Fish Fillets

  • Fengle Zhu
  • Derong Zhang
  • Yong He
  • Fei Liu
  • Da-Wen Sun
Communication

Abstract

The potential of visible and near infrared (VIS/NIR) hyperspectral imaging was investigated as a rapid and nondestructive technique to determine whether fish has been frozen–thawed. A total of 108 halibut (Psetta maxima) fillets were studied, including 48 fresh and 60 frozen–thawed (F-T) samples. Regarding the F-T samples, two speeds of freezing (fast and slow) were tested. The hyperspectral images of fillets were captured using a pushbroom hyperspectral imaging system in the spectral region of 380 to 1,030 nm. All images were calibrated for reflectance, followed by the minimum noise fraction rotation to reduce the noise. A region-of-interest (ROI) at the image center was selected, and the average spectral data were generated from the ROI image. Dimension reduction was carried out on the ROI image by principal component analysis. The first three principal components (PCs) explained over 98 % of variances of all spectral bands. Gray-level co-occurrence matrix analysis was implemented on the three PC images to extract 36 textural feature variables in total. Least squares-support vector machine classification models were developed to differentiate between fresh and F-T fish based on (1) spectral variables; (2) textural variables; (3) combined spectral and textural variables, respectively. Satisfactory average correct classification rate of 97.22 % for the prediction samples based on (3) was achieved, which was superior to the results based on (1) or (2). The results turned worse when different freezing rates were taken into consideration to classify three groups of fish. The overall results indicate that VIS/NIR hyperspectral imaging technique is promising for the reliable differentiation between fresh and F-T fish.

Keywords

Fresh fish Frozen–thawed fish Differentiation Hyperspectral imaging Least squares-support vector machine 

Notes

Acknowledgments

This study was supported by the 863 National High-Tech Research and Development Plan (Project no: 2011AA100705) and the Fundamental Research Funds for the Central Universities.

References

  1. Baixas-Nogueras, S., Bover-Cid, S., Veciana-Nogués, M. T., & Vidal-Carou, M. C. (2007). Effects of previous frozen storage on chemical, microbiological and sensory changes during chilled storage of Mediterranean hake (Merluccius merluccius) after thawing. European Food Research and Technology, 226, 287–293.CrossRefGoogle Scholar
  2. Benjakul, S., Visessanguan, W., Thongkaew, C., & Tanaka, M. (2003). Comparative study on physicochemical changes of muscle proteins from some tropical fish during frozen storage. Food Research International, 36, 787–795.CrossRefGoogle Scholar
  3. Chau, A., Whitworth, M., Leadley, C., & Millar, S. (2009). Innovative sensors to rapidly and non-destructively determine fish freshness. Campden BRI. Report No. CMS/REP/110284/1.Google Scholar
  4. Costa, C., D’Andrea, S., Russo, R., Antonucci, F., Pallottino, F., & Menesatti, P. (2011). Application of non-invasive techniques to differentiate sea bass (Dicentrarchus labrax, L. 1758) quality cultured under different conditions. Aquaculture International, 19, 765–778.Google Scholar
  5. Duflos, G., Le Fur, B., Mulak, V., Becel, P., & Malle, P. (2002). Comparison of methods of differentiating between fresh and frozen–thawed fish or fillets. Journal of the Science of Food and Agriculture, 82, 1341–1345.CrossRefGoogle Scholar
  6. ElMasry, G., & Wold, J. P. (2008). High-speed assessment of fat and water content distribution in fish fillets using online imaging spectroscopy. Journal of Agricultural and Food Chemistry, 56, 7672–7677.CrossRefGoogle Scholar
  7. Folkestad, A., Wold, J. P., Rørvik, K. A., Tschudi, J., Haugholt, K. H., Kolstad, K., & Mørkøre, T. (2008). Rapid and non-invasive measurements of fat and pigment concentrations in live and slaughtered Atlantic salmon (Salmo salar L.). Aquaculture, 280, 129–135.CrossRefGoogle Scholar
  8. Gao, X., & Tan, J. (1996). Analysis of expended-food texture by image processing part I: geometric properties. Journal of Food Processing Engineering, 19, 425–444.CrossRefGoogle Scholar
  9. Green, A. A., Berman, M., Switzer, P., & Craig, M. D. (1988). A transformation for ordering multispectral data in terms of image quality with implications for noise removal. IEEE Transactions on Geoscience and Remote Sensing, 26, 65–74.CrossRefGoogle Scholar
  10. Haralick, R. M., Shanmugam, K., & Dinstein, I. (1973). Textural features for image classification. IEEE Transactions on Systems, Man, and Cybernetics, 3, 610–621.CrossRefGoogle Scholar
  11. Johnson, R. A. (1998). Applied multivariate methods for data analysis. New York: Duxbury.Google Scholar
  12. Karoui, R., Thomas, E., & Dufour, E. (2006). Utilisation of a rapid technique based on front-face fluorescence spectroscopy for differentiating between fresh and frozen–thawed fish fillets. Food Research International, 39, 349–355.CrossRefGoogle Scholar
  13. Karoui, R., Lefur, B., Grondin, C., Thomas, E., Demeulemester, C., Baerdemaeker, J. D., & Guillard, A.-S. (2007). Mid-infrared spectroscopy as a new tool for the evaluation of fish freshness. International Journal of Food Science and Technology, 42, 57–64.CrossRefGoogle Scholar
  14. Li, B., & Sun, D. W. (2002). Novel methods for rapid freezing and thawing of foods—a review. Journal of Food Engineering, 54, 175–182.CrossRefGoogle Scholar
  15. Li, X. L., He, Y., & Wu, C. Q. (2008). Non-destructive discrimination of paddy seeds of different storage age based on Vis/NIR spectroscopy. Journal of Stored Products Research, 44, 264–268.CrossRefGoogle Scholar
  16. Menesatti, P., Costa, C., & Aguzzi, J. (2010). Quality evaluation of fish by hyperspectral imaging. In D.-W. Sun (Ed.), Hyperspectral imaging for food quality analysis and control (pp. 273–294). London: Academic.CrossRefGoogle Scholar
  17. Nilsen, H., Esaiassen, M., Heia, K., & Sigernes, F. (2002). Visible/near-infrared spectroscopy: a new tool for the evaluation of fish freshness? Journal of Food Science, 67, 1821–1826.CrossRefGoogle Scholar
  18. Osborne, B. G., & Fearn, T. (1986). Near infrared spectroscopy in food analysis. New York: Longman Scientific & Technical.Google Scholar
  19. Sivertsen, A. H., Chu, C. K., Wang, L. C., Godtliebsen, F., Heia, K., & Nilsen, H. (2009). Ridge detection with application to automatic fish fillet inspection. Journal of Food Engineering, 90, 317–324.CrossRefGoogle Scholar
  20. Sivertsen, A. H., Kimiya, T., & Heia, K. (2011). Automatic freshness assessment of cod (Gadus morhua) fillets by Vis/Nir spectroscopy. Journal of Food Engineering, 103, 317–323.CrossRefGoogle Scholar
  21. Suykens, J. A. K., Van Gestel, T., De Brabanter, J., De Moor, B., & Vandewalle, J. (2002). Least squares support vector machines. Singapore: World Scientific.CrossRefGoogle Scholar
  22. Uddin, M., & Okazaki, E. (2004). Classification of fresh and frozen–thawed fish by near-infrared spectroscopy. Journal of Food Science, 69, C665–C668.CrossRefGoogle Scholar
  23. Uddin, M., Okazaki, E., Turza, S., Yumiko, Y., Tanaka, M., & Fukuda, Y. (2005). Non-destructive visible/NIR spectroscopy for differentiation of fresh and frozen–thawed fish. Journal of Food Science, 70, C506–C510.CrossRefGoogle Scholar
  24. Xiccato, G., Trocino, A., Tulli, F., & Tibaldi, E. (2004). Prediction of chemical composition and origin identification of European sea bass (Dicentrarchus labrax L.) by near infrared reflectance spectroscopy (NIRS). Food Chemistry, 86, 275–281.CrossRefGoogle Scholar
  25. Zheng, C. X., Sun, D. W., & Zheng, L. Y. (2006). Recent applications of image texture for evaluation of food qualities—a review. Trends in Food Science and Technology, 17, 113–128.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Fengle Zhu
    • 1
  • Derong Zhang
    • 2
  • Yong He
    • 1
  • Fei Liu
    • 1
  • Da-Wen Sun
    • 3
  1. 1.College of Biosystems Engineering and Food ScienceZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Ningbo Institute of TechnologyZhejiang UniversityNingboChina
  3. 3.Food Refrigeration and Computerised Food TechnologyNational University of IrelandBelfieldIreland

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