Food and Bioprocess Technology

, Volume 6, Issue 8, pp 2202–2206 | Cite as

Optimized Extraction Method of Acetic Acid in Vinegar and Its Effect on SNIF-NMR Analysis to Control the Authenticity of Vinegar

  • Wen-Ching Ko
  • Ju-Yun Cheng
  • Po-Yen Chen
  • Chang-Wei Hsieh
Communication
First Online:
Received:
Accepted:

Abstract

Site-specific natural isotope fractionation by nuclear magnetic resonance (SNIF-NMR) is an accurate method for examining food adulteration. SNIF-NMR can be used to determine the ratio of deuterium to hydrogen (D/H) of a specific molecule position. Because the quantity of D differs between synthetic and fermented acetic acids, the method can be used to accurately monitor food adulteration. However, the effect of pretreated food on the results of SNIF-NMR has rarely been discussed. We present an extractive distillation method to increase the purity of acetic acid for fractionation; we used an orthogonal array experimental design to determine the optimal extraction conditions. We discuss the influence of extract solvents and sample concentration on the (D/H)CH3 values calculated from the NMR results. The optimal conditions for extracting acetic acid were found to be a sample-to-solvent ratio of 1:1, seven extractions, and an extraction time of 15 min. The extraction rates for acetic acid were 93.65% and 80.57% when ethyl acetate and diethyl ether, respectively, were used. The acetic acid concentration of the ethyl acetate- and diethyl ether-extracted samples was further improved by distillation from 5 g/100 mL to 33.84 and 51.65 g/100 mL, respectively.

Keywords

Extractive distillation 2H-NMR SNIF-NMR 
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Notes

Acknowledgments

This work was supported by the National Science Council (NSC) of the Republic of China (Taiwan) (no. NSC 96-2313-B-212-005-MY2).

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Wen-Ching Ko
    • 1
  • Ju-Yun Cheng
    • 1
  • Po-Yen Chen
    • 2
  • Chang-Wei Hsieh
    • 3
  1. 1.Department and Graduate Program of BioIndustry TechnologyDa-Yeh UniversityChanghuaRepublic of China
  2. 2.Sheng-Kang Biotechnology Corp. Ltd.Taichung CityRepublic of China
  3. 3.Department of Medicinal Botany and HealthcareDa-Yeh UniversityChanghuaRepublic of China

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